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The quantitative partition of the albumin fraction of milk serum proteins by gel chromatography

Published online by Cambridge University Press:  01 June 2009

D. T. Davies
D. T. Davies
Affiliation:
The Hannah Research Institute, Ayr, KA6 5HL
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Summary

A method for the quantitative partition of the albumin fraction of milk serum proteins is described. The partition, which is carried out by chromatography on a column of Sephadex G-100 (2·5×90cm) at 4°C in the presence of 0·02 m-sodium phosphate buffer (pH 6·7) containing 0·1 m-NaCl, yields 4 fractions. By gel electrophoresis it was shown that the fractions represent, in order of elution from the Sephadex, the following: fraction 1, a mixture of the largest proteins present, probably comprising γ-globulins, proteose-peptones and lactoferrin; fraction 2, bovine serum albumin and bovine serum transferrins; fraction 3, β-lactoglobulins; and fraction 4, α-lactalbumins. The protein contents of the various fractions were determined by nitrogen analysis and the results showed that good agreement was obtained between values from duplicate fractionations, and also that the recovery of material was satisfactory. The partition of the albumin fraction was not complete, but at least 90% of the material was found in fractions which represent essentially single proteins. The influences of some aspects of the pretreatment of samples, buffer composition and column preparation are described.

Type
Research Article
Information
Journal of Dairy Research , Volume 41 , Issue 2 , June 1974 , pp. 217 - 228
Copyright
Copyright © Proprietors of Journal of Dairy Research 1974

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References

REFERENCES

Abrams, A. & Baron, C. (1967). Biochemistry 6, 225.CrossRefGoogle Scholar
Armstrong, J. McD., Hopper, K. E., McKenzie, H. A. & Murphy, W. H. (1970). Biochimica et Biophysica Acta 214, 419.CrossRefGoogle Scholar
Armstrong, J. McD., McKenzie, H. A. & Sawyer, W. H. (1967). Biochimica et Biophysica Acta 147, 60.CrossRefGoogle Scholar
Aschaffenburg, R. (1968). Journal of Dairy Research 35, 447.CrossRefGoogle Scholar
Aschaffenburg, R. & Drewry, J. (1957). Biochemical Journal 65, 273.CrossRefGoogle Scholar
Aschaffenburg, R. & Drewry, J. (1959). 15th International Dairy Congress, London 3, 1631.Google Scholar
Hopper, K. E. & McKenzie, H. A. (1973). Biochimica et Biophysica Acta 295, 352.CrossRefGoogle Scholar
Jenness, R. (1970). In Milk Proteins: Chemistry and Molecular Biology, vol. 1, p. 31. (Ed. Mckenzie, H. A..) New York: Academic Press.Google Scholar
Larson, B. L. & Kendall, K. A. (1957). Journal of Dairy Science 40, 377.CrossRefGoogle Scholar
Larson, B. L. & Rolleri, G. D. (1955). Journal of Dairy Science 38, 351.CrossRefGoogle Scholar
Lontie, R. & Préaux, G. (1967). Industrie Chimique Belge 32 (III), 820.Google Scholar
Ng, W. C., Brunner, J. R. & Rhee, K. C. (1970). Journal of Dairy Science 53, 987.CrossRefGoogle Scholar
Préaux, G., De Weer, P., Peirsman, E., Tielemans, K. & Lontie, R. (1965). Archives Internationales de Physiologie et de Biochimie 73, 154.Google Scholar
Préaux, G. & Lontie, R. (1962). Protides of the Biological Fluids: 9th colloquium, 1961, p. 103.Google Scholar
Rolleri, G. D., Larson, B. L. & Touchberry, R. W. (1956). Journal of Dairy Science 39, 1683.CrossRefGoogle Scholar
Rowland, S. J. (1938). Journal of Dairy Research 9, 42.CrossRefGoogle Scholar
Smith, K. L., Conrad, H. R. & Porter, R. M. (1971). Journal of Dairy Science 54, 1427.CrossRefGoogle Scholar
Sweetsur, A. W. M. (1971). Ph.D. Thesis, University of Strathclyde.Google Scholar
Vanaman, T. C., Wakil, S. J. & Hill, R. L. (1968). Journal of Biological Chemistry 243, 6409.CrossRefGoogle Scholar
Watkin, J. E. & Miller, R. A. (1970). Analytical Biochemistry 34, 424.CrossRefGoogle Scholar
Yaguchi, M. & Rose, D. (1971). Journal of Dairy Science 54, 1725.CrossRefGoogle Scholar