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Supplemental fish oil does not alter immune competence or the pathophysiological response to an intramammary infusion of endotoxin in peri-partum multiparous Holstein cows

Published online by Cambridge University Press:  05 January 2009

Michael A Ballou ,
Rodrigo C Gomes  and
Edward J DePeters
Michael A Ballou*
Affiliation:
Department of Animal Science and Nutritional Biology Graduate Group, University of California at Davis95616
Rodrigo C Gomes
Affiliation:
Department of Animal Science and Nutritional Biology Graduate Group, University of California at Davis95616
Edward J DePeters
Affiliation:
Department of Animal Science and Nutritional Biology Graduate Group, University of California at Davis95616
*
†For correspondence; e-mail: Michael.Ballou@ttu.edu
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Abstract

The objective was to determine the effects of supplementing the diet with fish oil during the peri-partum period on the immune competence and the pathophysiological response to a lipopolysaccharide-induced mastitis challenge. Multiparous Holstein cows (n=30) were completely randomized to one of two treatments at 3 weeks pre-partum. Treatments differed only in the source of supplemental lipid and included either Energy Booster® or fish oil. Treatment diets were fed from −21 d relative to expected date of parturition until 10 d post partum. Treatments were fed as a bolus prior to the a.m. feeding. The dose of lipid during the pre-partum period was 250 g/d, whereas the amount of lipid supplemented post partum was adjusted to the level of intake, approximately 0·92% of the previous day's dry matter intake. Ex-vivo analyses of immune competence were measured including the antimicrobial activity of whole blood against Escherichia coli, Salmonella typhimurium and Candida albicans as well as the production of interferon-γ by peripheral blood mononuclear cultures. At 7 days in milk cows were infused with 100 μg of Esch. coli lipopolysaccharide into one rear quarter. Supplementing fish oil increased plasma concentrations of eicosapentaenoic and docosahexaenoic acids, but had no affect on the proportions of arachidonic acid at calving. Fish oil did not influence the production of interferon-γ or the antimicrobial activity of whole blood against any of the microorganisms. Furthermore, fish oil had no ameliorative effect on either the local or the systemic acute phase response following an intramammary lipopolysaccharide challenge in early lactating Holstein cows. Supplementing fish oil in the diet of peri-partum cows will not protect them from deleterious effects of an excessive acute phase response.

Keywords

Endotoxinfish oilmastitistransition
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 2 , May 2009 , pp. 165 - 172
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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References

Anderson, KL, Kindahl, H, Smith, AR, Davis, LE & Gustafsson, BK 1986 Endotoxin-induced bovine mastitis: Arachidonic acid metabolites in milk and plasma and effects on flunixin meglumine. American Journal of Veterinary Research 47 1373–1377Google ScholarPubMed
Anderson, MJ & Fritsche, KL 2004 Dietary polyunsaturated fatty acids modulate in vivo, antigen-driven CD4+ T-cell proliferation in mice. Journal of Nutrition 134 1978–1983Google Scholar
Ashes, JR, Siebert, BD, Gulati, SK, Cuthbertson, AZ & Scott, TW 1992 Incorporation of n-3 fatty acids of fish oil into tissue and serum lipids of ruminants. Lipids 27 629–631Google Scholar
Baguma-Nibasheka, M, Thomas Brenna, J & Nathanielsz, PW 1999 Delay of preterm delivery in sheep by omega-3 long chain polyunsaturates. Biology of Reproduction 60 698–701CrossRefGoogle ScholarPubMed
Burvenich, C & Peeters, G 1982 Effect of prostaglandin synthetase inhibitors on mammary blood flow during experimentally induced mastitis in lactating goats. Archives Internationales de Pharmacodynamie et de Thérapie 258 128–137Google ScholarPubMed
Calder, PC, Yaqoob, P, Thies, F, Wallace, FA & Miles, EA 2002 Fatty acids and lymphocyte functions. British Journal of Nutrition 87(Suppl. 1) S31–S48Google Scholar
Calder, PC 2006 n-3-Polyunsaturated fatty acids, inflammation, and inflammatory diseases. American Journal of Clinical Nutrition 83 1505S–1519SGoogle Scholar
Fritsche, K, Irons, R, Pompos, L, Janes, J, Zheng, Z & Brown, C 2005 Omega-3 polyunsaturated fatty acid impairment of early host resistance against Listeria monocytogenes infection is independent of neutrophil infiltration and function. Cellular Immunology 235 65–71CrossRefGoogle ScholarPubMed
Goldman, DW, Pickett, WC & Goetzl, EJ 1983 Human neutrophil chemotactic and degranulating activities of leukotriene B5 (LTB5) derived from eicosapentaenoic acid. Biochemical and Biophysical Research Communications 117 282–288CrossRefGoogle ScholarPubMed
Johnson, JA, Griswold, JA & Muakkassa, FF 1993 Essential fatty acids influence survival in sepsis. Journal of Trauma 35 128–131Google Scholar
Laidlaw, M & Holub, BJ 2003 Effects of supplementation with fish oil-derived n-3 fatty acids and γ-linolenic acid on circulating plasma lipids and fatty acid profiles in women. American Journal of Clinical Nutrition 77 37–42Google Scholar
Lee, TH, Hoover, RL, Williams, JD, Sperling, RI, Ravalese, J, Spur, BW, Robinson, DR, Corey, EJ, Lewis, RA & Austen, KF 1985 Effect of dietary enrichment with eicosapentaenoic and docosahexaenoic acids on in vitro neutrophil and monocytes leukotriene generation and neutrophil function. New England Journal of Medicine 312 1217–1224CrossRefGoogle ScholarPubMed
Lehtolainen, T, Suominen, S, Kutila, T & Pyörälä, S 2003 Effect of intramammary Escherichia coli endotoxin in early versus late-lactating dairy cows. Journal of Dairy Science 86 2327–2333Google Scholar
Lessard, M, Gagnon, N, Godson, DL & Petit, HV 2004 Influence of parturition and diets enriched with n-3 or n-6 polyunsaturated fatty acids on immune response of dairy cows during the transition period. Journal of Dairy Science 87 2197–2210CrossRefGoogle ScholarPubMed
Mascioli, J, Dohoo, IR & Duizer, G 1989 Endotoxin challenge after menhaden oil diet: effects on survival of guinea pigs. American Journal of Clinical Nutrition 49 277–282Google Scholar
Mattos, R, Staples, CR, Arteche, A, Wiltbank, MC, Diaz, FJ, Jenkins, TC & Thatcher, WW 2004 The effects of feeding fish oil on uterine secretion of PGE2α, milk composition, and metabolic status of periparturient Holstein cows. Journal of Dairy Science 87 921–932Google Scholar
Michaeli, B, Berger, MM, Revelly, JP, Tappy, L & Chioléro, R 2007 Effects of fish oil on the neuro-endocrine response to an endotoxin challenge in healthy volunteers. Clinical Nutrition 26 70–77CrossRefGoogle Scholar
Miles, EA, Banerjee, T & Calder, PC 2004 The influence of difference combinations of γ-linolenic, stearidonic, and eicosapentaenoic acids on the fatty acid composition of blood lipids and mononuclear cells in human volunteers. Prostaglandins Leukotrienes and Essential Fatty Acids 70 529–538CrossRefGoogle ScholarPubMed
Millet, S, Bennett, J, Lee, KA, Hau, M & Klasing, KC 2007 Quantifying and comparing constitutive immunity across avian species. Developmental and Comparative Immunology 31 188–201CrossRefGoogle ScholarPubMed
Novak, TE, Babcock, TA, Jho, DH, Helton, WS & Espat, NJ 2003 NF-κB inhibition by ω-3 fatty acids modulates LPS-stimulated macrophage TNF-α transcription. American Journal of Physiology – Lung Cellular and Molecular Physiology 284 L84–L89Google Scholar
Rees, D, Miles, EA, Banerjee, T, Wells, SJ, Roynette, CE, Wahle, KWJ & Calder, PC 2006 Dose-related effects of eicosapentaenoic acid on innate immune function in healthy humans: a comparison of young and older men. American Journal of Clinical Nutrition 83 331–342CrossRefGoogle Scholar
Schmidt, EB, Pedersen, JO, Varming, K, Ernst, E, Jersild, C, Grunnet, N & Dyerberg, J 1991 n-3-Fatty acids and leukocyte chemotaxis. Effects in hyperlipidemia and dose-response studies in healthy men. Arteriosclerosis and Thrombosis 11 429–435CrossRefGoogle ScholarPubMed
Shafer-Weaver, KA & Sordillo, LM 1997 Bovine CD8+ supporessor lymphocytes alter immune responsiveness during the post-partum period. Veterinary Immunology and Immunopathology 56 53–64CrossRefGoogle Scholar
Shapiro, AC, Wu, D & Meydani, SN 1993 Eicosanoids derived from arachidonic and eicosapentaenoic acids inhibit T cell proliferative response. Prostaglandins 45 229–240Google Scholar
Shuster, DE, Lee, EK & Kehrli, ME 1996 Bacterial growth, inflammatory cytokine production, and neutrophil recruitment during coliform mastitis in cows within ten days after calving, compared with cows at midlactation. American Journal of Veterinary Research 57 1569–1575Google Scholar
Sordillo, LM, Pighetti, GM & Davis, MR 1995 Enhanced production of bovine tumor necrosis factor-α during the periparturient period. Veterinary Immunololgy Immunopathololgy 49 263–270Google Scholar
Stulnig, TM, Berger, M, Sigmund, T, Raederstorff, D, Stockinger, H & Waldhäusl, W 1998 Polyunsaturated fatty acids inhibit T cell signal transduction by modification of detergent-insoluble membrane domains. Journal of Cellular Biology 143 637–644CrossRefGoogle ScholarPubMed
Vangroenweghe, F, Duchateau, L, Boutet, P, Lekeux, P, Rainard, P, Paape, MJ & Burvenich, C 2005 Effect of carprofen treatment following experimentally induced Escherichia coli mastitis in primiparous cows. Journal of Dairy Science 88 2361–2376Google Scholar
Yaqoob, P, Pala, HS, Cortina-Borja, M, Newsholme, EA & Calder, PC 2000 Encapsulated fish oil enriched in alpha-tocopherol alters plasma phospholipid and mononuclear cell fatty acid compositions but not mononuclear cell functions. European Journal of Clinical Investigation 30 260–274CrossRefGoogle Scholar