Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-19T14:35:29.377Z Has data issue: false hasContentIssue false

Comparative whey proteome analysis of small-tailed Han and DairyMeade ovine milk

Published online by Cambridge University Press:  06 December 2021

Urhan Bai
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China
Xiaohu Su*
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China
Zhong Zheng
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China
Liguo Zhang
Affiliation:
Ulanqab Animal Husbandry Workstation, Ulanqab Agriculture and Animal Husbandry Bureau, Ulanqab, Inner Mongolia Autonomous Region, 012000, People's Republic of China
Ying Ma
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China
Yingjie Dou
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China
Xiaoran Zhang
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China
Guanghua Su
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China
Ningcong Zhou
Affiliation:
Ulanqab Animal Husbandry Workstation, Ulanqab Agriculture and Animal Husbandry Bureau, Ulanqab, Inner Mongolia Autonomous Region, 012000, People's Republic of China
Guangpeng Li
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China
Li Zhang*
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, People's Republic of China
*
Author for correspondence: Li Zhang, Email: zhanglinmg@aliyun.com Xiaohu Su, Email: 13947144670@139.com
Author for correspondence: Li Zhang, Email: zhanglinmg@aliyun.com Xiaohu Su, Email: 13947144670@139.com

Abstract

We characterized the proteome profile of mid-lactation small-tailed Han (STH) and DairyMeade (DM) ovine milk in order to explore physiological variation and differences in milk traits between the two breeds. Methodology combined a tandem mass tag (TMT) proteomic approach with LC-MS/MS technology. A total of 656 proteins were identified in STH and DM ovine milk, of which 17and 29 proteins were significantly upregulated (P < 0.05) in STH and DM, respectively. Immune-related proteins and disease-related proteins were highly expressed in STH milk, whereas S100A2 and AEBP1 were highly expressed in DM milk, which had beneficial effects on mammary gland development and milk yield. Our results provide a theoretical basis for future breeding of dairy sheep.

Type
Research Article
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

These authors contributed equally to this project.

References

Auldist, MJ, Johnston, KA, White, NJ, Fitzsimons, WP and Boland, MJ (2004) A comparison of the composition, coagulation characteristics and cheesemaking capacity of milk from Friesian and Jersey dairy cows. Journal of Dairy Research 71, 5157.CrossRefGoogle ScholarPubMed
Balthazar, CF, Pimentel, TC, Ferrão, LL, Almada, CN, Santillo, A, Albenzio, M, Mollakhalili, N, Mortazavian, AM, Nascimento, JS, Silva, MC, Freitas, MQ, Sant Ana, AS, Granato, D and Cruz, AG (2017) Sheep milk: physicochemical characteristics and relevance for functional food development. Comprehensive Reviews in Food Science and Food Safety 16, 247262.CrossRefGoogle ScholarPubMed
Bhat, SA, Ahmad, SM, Ibeagha-Awemu, EM, Mobashir, M, Dar, MA, Mumtaz, PT, Shah, RA, Dar, TA, Shabir, N, Bhat, HF and Ganai, NA (2020) Comparative milk proteome analysis of Kashmiri and Jersey cattle identifies differential expression of key proteins involved in immune system regulation and milk quality. BMC Genomics 21, 161.CrossRefGoogle ScholarPubMed
Boehmer, JL, Ward, JL, Peters, RR, Shefcheck, KJ, McFarland, MA and Bannerman, DD (2010) Proteomic analysis of the temporal expression of bovine milk proteins during coliform mastitis and label-free relative quantification. Journal of Dairy Science 93, 593603.CrossRefGoogle ScholarPubMed
Cao, X, Song, D, Yang, M, Yang, N, Ye, Q, Tao, D, Liu, B, Wu, R and Yue, X (2017) Comparative analysis of whey N-glycoproteins in human colostrum and mature milk using quantitative glycoproteomics. Journal of Agricultural and Food Chemistry 65, 1036010367.CrossRefGoogle ScholarPubMed
Carrillo-Bustamante, P, Nguyen, THT, Oestereich, L, Günther, S, Guedj, J and Graw, F (2017) Determining Ribavirin's mechanism of action against Lassa virus infection. Scientific Reports 7, 11693–11612.CrossRefGoogle ScholarPubMed
Chatterton, DEW, Nguyen, DN, Bering, SB and Sangildm, PT (2013) Anti-inflammatory mechanisms of bioactive milk proteins in the intestine of newborns. The International Journal of Biochemistry & Cell Biology 45, 17301747.CrossRefGoogle ScholarPubMed
Christian, MP, Grainger, C, Sutherland, BJ, Mayes, JJ, Hannah, MC and Kefford, B (1999) Managing diet quality for cheddar cheese manufacturing milk. 2. Pasture v. Grain supplements. Journal of Dairy Research 66, 357363.CrossRefGoogle ScholarPubMed
Corrochano, AR, Buckin, V, Kelly, PM and Giblin, L (2018) Invited review: whey proteins as antioxidants and promoters of cellular antioxidant pathways. Journal of Dairy Science 101, 47474761.CrossRefGoogle ScholarPubMed
Haschke, F, Haiden, N and Thakkar, SK (2017) Nutritive and bioactive proteins in breastmilk. Annals of Nutrition & Metabolism 69(suppl 2), 1726.Google Scholar
Holloway, RW, Bogachev, O, Bharadwaj, AG, McCluskey, GD, Majdalawieh, AF, Zhang, L and Ro, H (2012) Stromal adipocyte enhancer-binding protein (AEBP1) promotes mammary epithelial cell hyperplasia via proinflammatory and hedgehog signaling. Journal of Biological Chemistry 287, 3917139181.CrossRefGoogle ScholarPubMed
Incassati, A, Chandramouli, A, Eelkema, R and Cowin, P (2010) Key signaling nodes in mammary gland development and cancer: beta-catenin. Breast Cancer Research: BCR 12, 213.CrossRefGoogle ScholarPubMed
Inman, JL, Robertson, C, Mott, JD and Bissell, MJ (2015) Mammary gland development: cell fate specification, stem cells and the microenvironment. Development 142, 10281042.CrossRefGoogle ScholarPubMed
Keragala, CB, Draxler, DF, McQuilten, ZK and Medcalf, RL (2018) Haemostasis and innate immunity – a complementary relationship: a review of the intricate relationship between coagulation and complement pathways. British Journal of Haematology 180, 782798.CrossRefGoogle ScholarPubMed
Li, S, Li, L, Zeng, Q, Liu, J, Yang, Y and Ren, D (2018) Quantitative differences in whey proteins among Murrah, Nili-Ravi and Mediterranean buffaloes using a TMT proteomic approach. Food Chemistry 269, 228235.CrossRefGoogle ScholarPubMed
Li, W, Li, M, Cao, X, Han, H, Kong, F and Yue, X (2020) Comparative analysis of whey proteins in donkey colostrum and mature milk using quantitative proteomics. Food Research International 127, 108741.CrossRefGoogle ScholarPubMed
Marais, AD (2019) Apolipoprotein E in lipoprotein metabolism, health and cardiovascular disease. Pathology 51, 165176.CrossRefGoogle ScholarPubMed
Minh, H, Manya, S, Elizabeth, D, Stewart, S and Peter, S (2015) In-Depth characterization of sheep (Ovis aries) milk whey proteome and comparison with Cow (Bos taurus)[J]. PLoS ONE 10, e0139774.Google Scholar
Mol, P, Kannegundla, U, Dey, G, Gopalakrishnan, L, Dammalli, M, Kumar, M, Patil, AH, Basavaraju, M, Rao, A, Ramesha, KP and Prasad, TSK (2018) Bovine milk comparative proteome analysis from early, mid, and late lactation in the cattle breed, malnad gidda (Bos indicus). OMICS: A Journal of Integrative Biology 22, 223235.CrossRefGoogle Scholar
Nishino, T (1994) The conversion of xanthine dehydrogenase to xanthine oxidase and the role of the enzyme in reperfusion injury. Journal of Biochemistry 116, 16.CrossRefGoogle ScholarPubMed
Park, YW, Juárez, M, Ramos, M and Haenlein, GFW (2007) Physico-chemical characteristics of goat and sheep milk. Small Ruminant Research 68(1-2), 88113.CrossRefGoogle Scholar
Scumaci, D, Trimboli, F, Dell'Aquia, L, Concolino, A, Pappaianni, G, Tamme, L, Vignola, G, Luciani, A, Morelli, D, Cuda, G, Boari, A and Britti, D (2015) Proteomics-driven analysis of ovine whey colostrum. PLoS ONE 10, e0117433.CrossRefGoogle ScholarPubMed
Sergio, G, and Ceballos, MP (2014) Mammaglobin A: review and clinical utility. Advances In Clinical Chemistry 64, 241268.Google Scholar
Speroni, L, Schaeberle, CM, Sonnenschein, C and Soto, AM (2018) Mammary gland development. Wiley Interdisciplinary Reviews. Developmental Biology 1, 533557.Google Scholar
Sun, Y, Wang, C, Sun, X and Guo, M (2020) Proteomic analysis of whey proteins in the colostrum and mature milk of Xinong Saanen goats. Journal of Dairy Science 103, 11641174.CrossRefGoogle ScholarPubMed
Tacoma, R, Fields, J, Ebenstein, DB, Lam, Y and Greenwood, SL (2016) Characterization of the bovine milk proteome in early-lactation Holstein and Jersey breeds of dairy cows. Journal of Proteomics 130, 200210.CrossRefGoogle ScholarPubMed
Turashvili, G, Bouchal, J, Burkadze, G and Kolar, Z (2007) Wnt signaling pathway in mammary gland development and carcinogenesis. Pathobiology 73, 213223.CrossRefGoogle Scholar
Walldius, G, Jungner, I, Holme, I, Aastveit, AH, Kolar, W and Steiner, E (2001) High apolipoprotein B, low apolipoprotein A-I, and improvement in the prediction of fatal myocardial infarction (AMORIS study): a prospective study. The Lancet 358(9298), 20262033.CrossRefGoogle ScholarPubMed
Wang, C, Zhang, C and Xing, X (2016) Xanthine dehydrogenase: an old enzyme with new knowledge and prospects. Bioengineered 7, 395405.CrossRefGoogle ScholarPubMed
Wang, Y, Li, Y, Ma, Y, Yang, Y, Ma, X, Li, X, Liu, F and Chen, B (2018) Association between apolipoprotein B genetic polymorphism and the risk of calcific aortic stenosis in Chinese subjects, in Xinjiang, China. Lipids in Health and Disease 17, 40.CrossRefGoogle ScholarPubMed
Yang, Y, Bu, D, Zhao, X, Sun, P, Wang, J and Zhou, L (2013) Proteomic analysis of cow, yak, buffalo, goat and camel milk whey proteins: quantitative differential expression patterns. Journal of Proteome Research 12, 16601667.CrossRefGoogle ScholarPubMed
Yu, Q, Verheyen, E and Zeng, Y (2016) Mammary development and breast cancer: a Wnt perspective. Cancers 8, 65.CrossRefGoogle ScholarPubMed
Zhang, L, Reidy, SP, Bogachev, O, Hall, BK, Majdalawieh, A and Ro, HS (2011) Lactation defect with impaired secretory activation in AEBP1-null mice. PLoS ONE 6, e27795.CrossRefGoogle ScholarPubMed
Zhang, X, Li, F, Qin, F, Li, W and Yue, X (2020) Exploration of ovine milk whey proteome during postnatal development using an iTRAQ approach. PeerJ 8, e10105.CrossRefGoogle ScholarPubMed
Supplementary material: PDF

Bai et al. supplementary material

Bai et al. supplementary material

Download Bai et al. supplementary material(PDF)
PDF 1.5 MB