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Detection and identification of Lactobacillus helveticus bacteriophages by PCR

Published online by Cambridge University Press:  12 May 2008

Miriam Zago ,
Lia Rossetti ,
Jorge Reinheimer ,
Domenico Carminati  and
Giorgio Giraffa
Miriam Zago
Affiliation:
CRA-FLC Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie, Lodi (Italy)
Lia Rossetti
Affiliation:
CRA-FLC Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie, Lodi (Italy)
Jorge Reinheimer
Affiliation:
Instituto de Lactología Industrial, Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe (Argentina)
Domenico Carminati
Affiliation:
CRA-FLC Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie, Lodi (Italy)
Giorgio Giraffa*
Affiliation:
CRA-FLC Centro di Ricerca per le Produzioni Foraggere e Lattiero Casearie, Lodi (Italy)
*
*For correspondence; e-mail: ggiraffa@ilclodi.it
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Abstract

A PCR protocol for detection of Lactobacillus helveticus bacteriophages was optimized. PCR was designed taking into account the sequence of the lys gene of temperate bacteriophage Φ-0303 and optimized to obtain a fragment of 222 bp using different Lb. helveticus phages from our collection. PCR was applied to total phage DNA extracted from 53 natural whey starters used for the production of Grana cheese and all gave the expected fragment. The presence of actively growing phages in the cultures was verified by traditional tests. Several PCR products of the lys gene were sequenced and aligned. The resulting sequences showed variable heterogeneity between the phages.

Keywords

Lactobacillus helveticuslytic bacteriophagesendolysin genePCRGrana cheese
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 2 , May 2008 , pp. 196 - 201
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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