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Effects of intraruminal infusion of propionate on the concentrations of ammonia and insulin in peripheral blood of cows receiving an intraruminal infusion of urea

Published online by Cambridge University Press:  01 June 2009

Jai-Jun Choung  and
David G. Chamberlain
Jai-Jun Choung
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
David G. Chamberlain
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
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Summary

To test the hypothesis that propionate can reduce hepatic capacity to detoxify ammonia, effects of the inclusion of propionate in intraruminal infusions of urea on the concentrations of ammonia, other metabolites and insulin in peripheral blood were investigated in two experiments with non-lactating dairy cows. Both experiments were of a 4 × 4 Latin square design with four animals, four treatments and four experimental periods of 7 d; feed was given in two equal meals each day, all intraruminal infusions were given for 1 h at the time of the morning feed, and propionic acid was partly neutralized with NaOH. In Expt 1, the treatments were a basal diet of pelleted lucerne and chopped hay alone or with the following infusions (g/d): urea 80, propionic acid 350, urea 80 plus propionic acid 350. The inclusion of propionate in the urea infusion markedly increased (P < 0·001) the concentration of ammonia in plasma compared with infusion of urea alone. Moreover, the inclusion of urea with the propionate infusion abolished (P < 0·01) the increase in blood insulin level seen with the infusion of propionate alone. In Expt 2, less severe treatments were imposed, the aim being to reproduce metabolic loads of propionate and ammonia that might be expected from a diet of high-protein grass silage rich in lactic acid. The treatments were a basal diet of grass silage alone or with the following infusions (g/d): NaCl 145, NaCl 145 plus urea 50, propionic acid 200, urea 50 plus propionic acid 200. Effects were less pronounced than in Expt 1 but, in the period immediately after infusion, similar effects were seen. It is concluded that propionate–ammonia interactions may have potentially important effects on milk production especially for diets with high proportions of grass silage containing high levels of protein and lactic acid.

Type
Original Articles
Information
Journal of Dairy Research , Volume 62 , Issue 4 , November 1995 , pp. 549 - 557
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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References

REFERENCES

Agricultural Research Council 1980 The Nutrient Requirements of Ruminant Livestock: Technical Review, 2nd edn. Farnham Royal: Commonwealth Agricultural BureauxGoogle Scholar
Barej, W. 1986 On the extent of ureagenesis and gluconeogenesis in ruminants with regard to NPN diets. Archives of Animal Nutrition 36 154–163Google Scholar
Barej, W., Harmeyer, J., Drost, H. & Libau, H. 1982 The effect of hyperammonaemia on plasma glucose, insulin, glucagon, and adrenalin levels in sheep. Zentralblatt für Veterinärmedizin A29 197–206Google Scholar
Chamberlain, D. G. & Thomas, P. C. 1979 Ruminal nitrogen metabolism and the passage of amino acids to the duodenum in sheep receiving diets containing hay and concentrates in varying proportions. Journal of the Science of Food and Agriculture 30 677–686CrossRefGoogle Scholar
Chamberlain, D. G., Thomas, P. C. & Anderson, F. J. 1983 Volatile fatty acid proportions and lactic acid metabolism in the rumen in sheep and cattle receiving silage diets. Journal of Agricultural Science 101 47–58CrossRefGoogle Scholar
Chamberlain, D. G., Thomas, P. C. & Quig, J. 1986 Utilization of silage nitrogen in sheep and cows: amino acid composition of duodenal digesta and rumen microbes. Grass and Forage Science 41 31–38 (EAAP Publication no. 16)CrossRefGoogle Scholar
Choung, J. -J., Chamberlain, D. G., Thomas, P. C. & Bradbury, I. 1990 The effects of intraruminal Infusions of urea on the voluntary intake and milk production of cows receiving grass silage diets. Journal of Dairy Research 57 455–464CrossRefGoogle ScholarPubMed
De Jong, A. 1982 Patterns of plasma concentrations of insulin and glucagon after intravascular and intraruminal administration of volatile fatty acids in the goat. Journal of Endocrinology 92 357–370CrossRefGoogle ScholarPubMed
Gill, M., Siddons, R. C., Beever, D. E. & Rowe, J. B. 1986 Metabolism of lactic acid isomers in the rumen of silage-fed sheep. British Journal of Nutrition 55 399–407CrossRefGoogle ScholarPubMed
Glasgow, A. M. & Chase, H. P. 1976 Effect of pent-4-enoic acid, propionic acid and other short-chain fatty acids on citrulline synthesis in rat liver mitochondria. Biochemical Journal 156 301–307CrossRefGoogle ScholarPubMed
Jenkins, T. C. & Thonney, M. L. 1988 Effect of propionate level in a volatile fatty acid salt mixture fed to lambs on weight gain, body composition and plasma metabolites. Journal of Animal Science 66 1028–1035CrossRefGoogle Scholar
Kelly, N. C. & Thomas, P. C. 1978 The nutritive value of silages. Energy metabolism in sheep receiving diets of grass silage or grass silage and barley. British Journal of Nutrition 40 205–219CrossRefGoogle ScholarPubMed
Leenanuruksa, D., Niumsup, P. & McDowell, G. H. 1988 Insulin affects glucose uptake by muscle and mammary tissues of lactating ewes. Australian Journal of Biological Science 41 453–461CrossRefGoogle ScholarPubMed
McDonald, P. & Edwards, R. A. 1976 Influence of conservation methods on digestion and utilization of forages by ruminants. Proceedings of the Nutrition Society 35 201–211CrossRefGoogle ScholarPubMed
Martin-Requero, A., Corkey, B. E., Cerdan, S., Walajtys-Rode, E., Parrilla, R. L. & Williamson, J. R. 1983 Interactions between α-ketoisovalerate metabolism and the pathways of gluconeogenesis and urea synthesis in isolated hepatocytes. Journal of Biological Chemistry 258 3673–3681CrossRefGoogle ScholarPubMed
Meijer, A. J., Lamers, W. H. & Chamuleau, R. A. F. M. 1990 Nitrogen metabolism and ornithine cycle function. Physiological Reviews 70 701–748CrossRefGoogle ScholarPubMed
Morgan, D. J. & L'Estrange, J. L. 1976 Effect of dietary additions of hydrochloric and lactic acid on feed intake and metabolism of sheep and cattle. Irish Journal of Agricultural Research 15 55–63Google Scholar
Morgan, D. J. & L'Estrange, J. L. 1977 Voluntary feed intake and metabolism of sheep when lactic acid is administered in the feed or intraruminally. Journal of the British Grassland Society 32 217–224CrossRefGoogle Scholar
Newbold, C. J., Chamberlain, D. G. & Williams, A. G. 1986 The effects of defaunation on the metabolism of lactic acid in the rumen. Journal of the Science of Food and Agriculture 37 1083–1090CrossRefGoogle Scholar
Orzechowski, A., Pierzynowski, S., Motyl, T. & Barej, W. 1988 Net hepatic metabolism of ammonia, propionate and lactate in sheep in relation to gluconeogenesis and ureagenesis. Journal of Animal Physiology and Animal Nutrition 59 113–122CrossRefGoogle Scholar
Payne, R. W., Lane, P. W., Ainsley, H. E., Bricknell, K. E., Digby, P. G. N., Harding, S. A., Leech, P. K., Simpson, H. R., Todd, A. P., Verrier, P. J. & White, R. P. 1987 Genstat 5 Reference Manual. Oxford: Clarendon PressGoogle Scholar
Rattenbury, J. M., Kenwright, A. M., Withers, C. J. & Shepherd, D. A. L. 1983 Effect of propionic acid on urea synthesis by sheep liver. Research in Veterinary Science 35, 61–64CrossRefGoogle ScholarPubMed
Reynolds, P. J. & Huntington, G. B. 1988 Net portal absorption of volatile fatty acids and L(+)-lactate by lactating Holstein cows. Journal of Dairy Science 71 124–133CrossRefGoogle ScholarPubMed
Siddons, R. C., Evans, R. T. & Beever, D. E. 1979 Effect of formaldehyde treatment before ensiling on the digestion of wilted grass silage by sheep. British Journal of Nutrition 42 535–545CrossRefGoogle ScholarPubMed
Sutton, J. D., Hart, I. C., Morant, S. V., Schuller, E. & Simmonds, A. D. 1988 Feeding frequency for lactating cows: diurnal patterns of hormones and metabolites in peripheral blood in relation to milk-fat concentration. British Journal of Nutrition 60 265–274CrossRefGoogle ScholarPubMed
Thomas, C. & Thomas, P. C. 1985 Factors affecting the nutritive value of grass silages. In Recent Advances in Animal Nutrition—1985, pp. 223–256 (Ed. Haresign, W.). London: ButterworthsCrossRefGoogle Scholar
Vernon, R. G. 1988 The partition of nutrients during the lactation cycle. In Nutrition and Lactation in the Dairy Cow, pp. 32–52 (Ed. Garnsworthy, P. C.). London: Butterworths (University of Nottingham Easier School in Agricultural Science 46)CrossRefGoogle Scholar