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Metabolomic analysis of alterations in lipid oxidation, carbohydrate and amino acid metabolism in dairy goats caused by exposure to Aflotoxin B1

Published online by Cambridge University Press:  20 November 2017

Jianbo Cheng ,
Shuai Huang ,
Caiyun Fan ,
Nan Zheng ,
Yangdong Zhang ,
Songli Li  and
Jiaqi Wang
Jianbo Cheng
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, People's Republic of China State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China
Shuai Huang
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, People's Republic of China
Caiyun Fan
Affiliation:
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, People's Republic of China
Nan Zheng
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China Ministry of Agriculture-Milk Risk Assessment Laboratory, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China
Yangdong Zhang
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China Ministry of Agriculture-Milk Risk Assessment Laboratory, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China
Songli Li
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China Ministry of Agriculture-Milk Risk Assessment Laboratory, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China
Jiaqi Wang*
Affiliation:
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China Ministry of Agriculture-Milk Risk Assessment Laboratory, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China
*
*For correspondence; e-mail: jiaqiwang@vip.163.com
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Abstract

The purposes of this study were to investigate the systemic and characteristic metabolites in the serum of dairy goats induced by aflatoxin B1 (AFB1) exposure and to further understand the endogenous metabolic alterations induced by it. A nuclear magnetic resonance (NMR)-based metabonomic approach was used to analyse the metabolic alterations in dairy goats that were induced by low doses of AFB1 (50 µg/kg DM). We found that AFB1 exposure caused significant elevations of glucose, citrate, acetate, acetoacetate, betaine, and glycine yet caused reductions of lactate, ketone bodies (acetate, β-hydroxybutyrate), amino acids (citrulline, leucine/isoleucine, valine, creatine) and cell membrane structures (choline, lipoprotein, N-acetyl glycoproteins) in the serum. These data indicated that AFB1 caused endogenous metabolic changes in various metabolic pathways, including cell membrane-associated metabolism, the tricarboxylic acid cycle, glycolysis, lipids, and amino acid metabolism. These findings provide both a comprehensive insight into the metabolic aspects of AFB1-induced adverse effects on dairy goats and a method for monitoring dairy animals exposed to low doses of AFB1.

Keywords

Aflatoxin B1metabolicNMRdairy goats
Type
Research Article
Information
Journal of Dairy Research , Volume 84 , Issue 4 , November 2017 , pp. 401 - 406
Copyright
Copyright © Hannah Research Foundation 2017 

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Footnotes

†

These authors contributed equally to this work.

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