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Optimisation of medium composition for probiotic biomass production using response surface methodology

Published online by Cambridge University Press:  19 December 2013

Masumeh Anvari ,
Gholam Khayati  and
Shora Rostami
Masumeh Anvari*
Affiliation:
Department of Microbiology, Faculty of Sciences, Islamic Azad University, Rasht Branch, P.O. Box 41335-3516, Rasht, Iran
Gholam Khayati
Affiliation:
Department of Chemical Engineering, Faculty of Eng., Guilan University, P. O. Box 3756-41635, Rasht, Iran
Shora Rostami
Affiliation:
Department of Microbiology, Sciences and Research Branch, Islamic Azad University, Guilan, Iran
*
*Corresponding author; e-mail: anvari@iaurasht.ac.ir
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Abstract

This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp. lactis in apple juice and to assess the effects of these factors by using response surface methodology. A second-order central composite design was applied to evaluate the effects of these independent variables on growth of the microorganism. A polynomial regression model with cubic and quadratic terms was used for analysis of the experimental data. It was found that the effects involving inulin, yeast extract and pH on growth of the bacterium were significant, and the strongest effect was given by the yeast extract concentration. Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.

Keywords

Bifidobacterium animalis subsp. Lactisprobioticresponse surface methodologyapple juice
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 1 , February 2014 , pp. 59 - 64
Copyright
Copyright © Proprietors of Journal of Dairy Research 2013 

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