Hostname: page-component-7479d7b7d-rvbq7 Total loading time: 0 Render date: 2024-07-12T11:28:16.404Z Has data issue: false hasContentIssue false
  • English
  • Français

Polyclonal antibodies with predetermined specificity against bovine αs1-casein: application to the detection of bovine milk in ovine milk and cheese

Published online by Cambridge University Press:  01 June 2009

Marie-Paule Rolland ,
Lotfi Bitri  and
Pierre Besançon
Marie-Paule Rolland
Affiliation:
Unité de Nutrition, Génie Biologique et Sciences des Aliments, Département Agroressources et Procédés Biologique, Université de Montpellier II, 34095 Montpellier Cedex 05, France
Lotfi Bitri
Affiliation:
Unité de Nutrition, Génie Biologique et Sciences des Aliments, Département Agroressources et Procédés Biologique, Université de Montpellier II, 34095 Montpellier Cedex 05, France
Pierre Besançon
Affiliation:
Unité de Nutrition, Génie Biologique et Sciences des Aliments, Département Agroressources et Procédés Biologique, Université de Montpellier II, 34095 Montpellier Cedex 05, France
Get access Rights & Permissions [Opens in a new window]

Summary

Comparing the primary sequences of bovine and ovine milk proteins, some short peptide fragments are cow-specific, in particular the 141–148 fragment of bovine αs1-casein, which is deleted in its ovine counterpart. The 140–149 peptide was chemically synthesized on a solid phase matrix and directly used as an immunogen to produce polyclonal monospecific antibodies in rabbits. These antibodies recognized this fragment both on the peptidyl resin and in the native protein. They appeared to be monospecific, since no antigen–antibody complex was formed with homologous ovine or caprine proteins. Subsequently, a competitive enzyme-linked immuno-sorbent assay was successfully developed for the detection of defined amounts of cows' milk in sheep's milk from 0·125 to 64% (v/v) and in cheese from 0·5 to 25% (v/v) that was not influenced by heat treatment of milk or the degree of ripening of cheese.

Type
Original Articles
Information
Journal of Dairy Research , Volume 60 , Issue 3 , August 1993 , pp. 413 - 420
Copyright
Copyright © Proprietors of Journal of Dairy Research 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Addeo, F., Anelli, G. & Chianese, L. 1986 Gel isoelectrio focusing of cheese proteins to detect milk from different species in mixture. International Dairy Federation Bulletin Document 202 191–192Google Scholar
Addeo, F., Moio, L., Chianese, L. & Di Luccia, A. 1989 [Detection of bovine milk in ovine milk or cheese by gel isoelectric focusing of β-lactoglobulin: applications and limitations.] Italian Journal of Food Science 1 (1) 45–52Google Scholar
Addeo, F., Moio, L., Chianese, L., Stingo, C. & Di Luccia, A. 1990 Improved procedure for detecting bovine and ovine milk mixtures in cheese by isoelectric focusing of para-K-casein. Milchwissenschaft 45 221–224Google Scholar
Amigo, L., Ramos, M., Martin-Alvarez, P. J. & Barbosa, M. 1991 Effect of technological parameters on electrophoretic detection of cow's milk in ewe's milk cheeses. Journal of Dairy Science 74 1482–1490CrossRefGoogle Scholar
Aranda, P., Oria, R. & Calvo, M. 1988 Detection of cows' milk in ewes' and cheese by an immunodotting method. Journal of Dairy Research 55 121–124CrossRefGoogle ScholarPubMed
Assenat, L. 1967 [The identification of milks and cheeses by polyacrylamide gel electrophoresis.] Lait 47 393–414, 496–502CrossRefGoogle Scholar
Brignon, G., Mahe, M. F., Grosclaude, F. & Ribadeau-Dumas, B. 1989 Sequence of caprine αs1-casein and characterization of those of its genetic variants which are synthesized at a high level, αs1-CnA, B and C. Protein Sequences & Data Analysis 2 181–188Google Scholar
Calas, B., Mery, J., Parello, J. & Cave, A. 1985 Solid-phase synthesis using a new polyacrylic resin. Synthesis of the fragment 14–21 of the amino acid sequence of histone H4. Tetrahedron 41 5331–5339CrossRefGoogle Scholar
Calvo, M. M., Amigo, L., Olano, A., Martin, P. J. & Ramos, M. 1989 Effect of thermal treatments on the determination of bovine milk added to ovine or caprine milk. Food Chemistry 32 99–108CrossRefGoogle Scholar
Durand, M., Meusnier, M., Delahaye, J. & Prunet, P. 1974 [Detection of cows' milk in goats' and ewes' milk by an immunodiffusion method.] Bulletin de l' Académie Vétérinaire de France 47 247–258CrossRefGoogle Scholar
Elbertzhagen, H. 1987 [Detection of bovine casein in sheep's and goats' milk cheeses by immuno-electrophoresis.] Zeitschrift für Lebensmittel-Untersuchung und -Forschung 185 357–361CrossRefGoogle Scholar
García, T., Martín, R., Rodríguez, E., Morales, P., Hernández, P. & Sanz, B. 1990 Detection of bovine milk in ovine milk by an indirect enzyme-linked immunosorbent assay. Journal of Dairy Science 73 1489–1493CrossRefGoogle Scholar
Gatusso, A. M. & Fazio, G. 1980 [Characteristics and composition of milk fat from Sicilian breeds of sheep.] Rivista Ilaliana delle Sostanze Grasse 57 530–535Google Scholar
Iverson, J. L. & Sheppard, A. J. 1985 Utilization of capric and lauric acid ratios to detect substitution of cow's milk in imported sheep and goat cheeses. Journal of Dairy Science 68 (Suppl. 1) 95Google Scholar
Iverson, J. L. & Sheppard, A. J. 1989 Detection of adulteration in cow, goat and sheep cheeses utilizing gas-liquid chromatographic fatty acid data. Journal of Dairy Science 72 1707–1712CrossRefGoogle Scholar
Kaiser, K. P. & Krause, I. 1985 [Analysis of proteins in foods by means of electrophoretic and chromatographic methods.] Zeitschrift für Lebensmittel-Untersuchung und -Forschung 180 181–201CrossRefGoogle ScholarPubMed
Kennedy, R. C., Dreesman, G. R., Chanh, T. C., Boswell, R. N., Allan, J. S., Lee, T.-H., Essex, M., Sparrow, J. T., Ho, D. D. & Kanda, P. 1987 Use of a resin-bound synthetic peptide for identifying a neutralizing antigenic determinant associated with the human immunodeficiency virus envelope. Journal of Biological Chemistry 262 5769–5774CrossRefGoogle ScholarPubMed
Krause, I., Belitz, H. D. & Kaiser, K. P. 1982 [Detection of cow's milk in sheep's and goat's milk and cheese by isoelectric focusing on thin layers of polyacrylamide gels containing urea.] Zeitschrift für Lebensmittel-Untersuchung und -Forschung 174 195–199CrossRefGoogle Scholar
Laezza, P., Nota, G. & Addeo, F. 1991 Determination of bovine and ovine milk in mixtures by fast ion-exchange chromatography of whey proteins. Milchwissenschaft 46 559–561Google Scholar
Levieux, D. 1977 [New technique for detecting adulteration of goat's and ewe's milk.] Dossiers de l'Élevage 2 (2) 37–46Google Scholar
Levieux, D. 1980 The development of a rapid and sensitive method based on haemagglutination inhibition for the measurement of cow milk in goat milk. Annales de Recherches Veterinaires 11 151–156Google ScholarPubMed
Mercier, J. C., Grosclaude, F. & Ribadeau-Dumas, B. 1971 [Primary structure of bovine asl-casein. Complete sequence.] European Journal of Biochemistry 23 41–51CrossRefGoogle Scholar
Mercier, J. C., Maubois, J. L., Poznański, S. & Ribadeau-Dumas, B. 1968 [Preparative fractionation of bovine and ovine caseins by DEAE-cellulose chromatography in a medium containing urea and 2-mercaptoethanol.] Bulletin de la Société de Chimie Biologigue 50 521–530Google Scholar
Radford, D. V., Tchan, Y. T. & McPhillips, J. 1981 Detection of cow's in goat's milk by immuno-electrophoresis. Australian Journal of Dairy Technology 36 144–146Google Scholar
Ramos, M. & Juarez, M. 1984 Update on existing analytical methods for detecting mixtures of cow's, ewe's and goat's milk. International Dairy Federation Bulletin Document no. 181 3–9Google Scholar
Ramos, M., Martinez-Castro, I. & Juarez, M. 1977 Detection of cow's milk in Manchego cheese. Journal of Dairy Science 60 870–877CrossRefGoogle Scholar
Rispoli, S., Rivemale, M. & Saugues, R. 1991 [Identification and quantitative determination of cows' milk in ewes' cheeses using isoelectric focusing of wheys: application to cheeses at high ripening stage: Roquefort cheeses.] Lait 71 501–510.CrossRefGoogle Scholar
Rodríguez, E., Martín, R., García, T., Hernández, P. E. & Sanz, B. 1990 Detection of cows' milk in ewes' milk and cheese by an indirect enzyme-linked immunosorbent assay (ELISA). Journal of Dairy Research 57 197–205CrossRefGoogle ScholarPubMed
Shi, P. T., Riehm, J. P., Todd, P. E. E. & Leach, S. J. 1984 The antigenicity of myoglobine-related peptides synthesized on polyacrylamide and polystyrene resin supports. Molecular Immunology 21 489–496CrossRefGoogle ScholarPubMed
Smith, J. A., Hurrell, J. G. R. & Leach, S. J. 1977 A novel method for delineating antigenic determinants: peptide synthesis and radioimmunoassay using the same solid support. Immunochemistry 14 565–568CrossRefGoogle ScholarPubMed
Sutcliffe, J. G., Shinnick, T. M., Green, N. & Lerner, R. A. 1983 Antibodies that react with predetermined sites on proteins. Science 219 660–666CrossRefGoogle ScholarPubMed