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Variation in the Toll-like Receptor 4 (TLR4) gene affects milk traits in dairy cows

Published online by Cambridge University Press:  20 November 2017

Huitong Zhou ,
Long Cheng ,
Hua Gong ,
Seung O. Byun ,
Grant R. Edwards  and
Jon G. H. Hickford
Huitong Zhou
Affiliation:
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
Long Cheng
Affiliation:
Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Dookie Campus, Victoria 3647, Australia
Hua Gong
Affiliation:
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
Seung O. Byun
Affiliation:
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
Grant R. Edwards
Affiliation:
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
Jon G. H. Hickford*
Affiliation:
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
*
*For correspondence; e-mail: Jon.Hickford@lincoln.ac.nz
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Abstract

The objective of this Research Communication was to use polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analysis to investigate a region of the bovine TLR4 gene (TLR4) in pasture-fed New Zealand (NZ) Holstein-Friesian × Jersey (HF × J) cross dairy cows and to determine whether gene variation was associated with milk production traits. Genetic variation was observed, with two variants (A and B) containing a single nucleotide polymorphism (SNP) (c.2021C/T) that was non-synonymous and putatively results in a p.Thr674Ile substitution in the transmembrane/cytoplasmic domain of TLR4. Variant A was associated with higher milk yields, but lower milk fat percentages, whereas B was associated with lower milk yields, but higher fat and protein percentages. Cows of genotype AA produced more milk than AB or BB cows, but the milk produced by AA cows contained less fat than AB or BB cows.

Keywords

Toll-like receptor 4 gene (TLR4)milk production traitssingle nucleotide polymorphism (SNP)
Type
Research Article
Information
Journal of Dairy Research , Volume 84 , Issue 4 , November 2017 , pp. 426 - 429
Copyright
Copyright © Hannah Research Foundation 2017 

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