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Activation of the NLRP3 and AIM2 inflammasomes in a mouse model of Schistosoma mansoni infection

Published online by Cambridge University Press:  15 August 2019

T.T.W. Chen ,
P.C. Cheng ,
K.C. Chang ,
J.P. Cao ,
J.L. Feng ,
C.C. Chen ,
H.Y.P. Lam  and
S.Y. Peng Open the ORCID record for S.Y. Peng [Opens in a new window]
T.T.W. Chen
Affiliation:
Department of Biochemistry, School of Medicine, Tzu Chi University, Hualien 97004, Taiwan National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, MOH, Shanghai 200025, China
P.C. Cheng
Affiliation:
Department of Parasitology and Center for International Tropical medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan
K.C. Chang
Affiliation:
Institute of Laboratory Medicine and Biotechnology, College of Medicine, Tzu Chi University, Hualien 97004, Taiwan
J.P. Cao
Affiliation:
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, MOH, Shanghai 200025, China
J.L. Feng
Affiliation:
Institute of Laboratory Medicine and Biotechnology, College of Medicine, Tzu Chi University, Hualien 97004, Taiwan Department of Laboratory, Chung-Kang Branch, Cheng-Ching General Hospital, Taichung 40764, Taiwan
C.C. Chen
Affiliation:
Institute of Laboratory Medicine and Biotechnology, College of Medicine, Tzu Chi University, Hualien 97004, Taiwan Department of Laboratory Medicine, Taipei Medical University-Shuang-Ho Hospital, New Taipei City 23561, Taiwan
H.Y.P. Lam
Affiliation:
Department of Biochemistry, School of Medicine, Tzu Chi University, Hualien 97004, Taiwan
S.Y. Peng*
Affiliation:
Department of Biochemistry, School of Medicine, Tzu Chi University, Hualien 97004, Taiwan
*
Author for correspondence: S.Y. Peng, E-mail: pengsy@mail.tcu.edu.tw
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Abstract

Schistosomiasis is an inflammatory disease that occurs when schistosome species eggs are deposited in the liver, resulting in fibrosis and portal hypertension. Schistosomes can interact with host inflammasomes to elicit host immune responses, leading to mitochondrial damage, generation of high levels of reactive oxygen species (ROS) and activation of apoptosis during inflammation. This study aims to examine whether ROS and NF-κB (p65) expression elicited other types of inflammasome activation in Schistosoma mansoni-infected mouse livers. We examine the relationship between inflammasome activation, mitochondrial damage and ROS production in mouse livers infected with S. mansoni. We demonstrate a significant release of ROS and superoxides and increased NF-κB (p65) in S. mansoni-infected mouse livers. Moreover, activation of the NLRP3 and AIM2 inflammasomes was triggered by S. mansoni infection. Stimulation of HuH-7 hepatocellular carcinoma cells with soluble egg antigen induced activation of the AIM2 inflammasome pathway. In this study, we demonstrate that S. mansoni infection promotes both NLRP3 and AIM2 inflammasome activation.

Keywords

InflammasomeschistosomesROSSEA
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e72
Copyright
Copyright © Cambridge University Press 2019 

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Footnotes

*

These authors contributed equally to this work

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