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Anisakis simplex: stage-specific antigens recognized by mice

Published online by Cambridge University Press:  05 June 2009

R. Iglesias ,
J. Leiro ,
F.M. Ubeira ,
M.T. Santamarina  and
M.L. Sanmartín
R. Iglesias
Affiliation:
Instituto de Investigación y Análisis Alimentario, Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain
J. Leiro
Affiliation:
Instituto de Investigación y Análisis Alimentario, Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain
F.M. Ubeira
Affiliation:
Instituto de Investigación y Análisis Alimentario, Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain
M.T. Santamarina
Affiliation:
Instituto de Investigación y Análisis Alimentario, Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain
M.L. Sanmartín*
Affiliation:
Instituto de Investigación y Análisis Alimentario, Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Spain
*
*Author for correspondence
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Abstract

To investigate antigenic differences between the developmental stages of Anisakis simplex, somatic, excretion–secretion and detergent-solubilized surface antigen preparations of third- and fourth-stage A. simplex larvae, and a somatic antigen preparation of adults, were characterized by SDS–PAGE and immunoblotting using mouse immune serum containing antibodies against the two larval stages. Excretion–secretion and surface antigen preparations from thirdstage larvae behaved very differently from those of fourth-stage larvae, in both SDS-PAGE and immunoblotting; this suggests that certain excretion–secretion and surface antigens are highly stage-specific. By contrast, somatic components of third- and fourth-stage larvae were found to have very similar banding patterns, suggesting conservation of these components during the development of the parasite in mice. The SDS-PAGE and immunoblotting results for adult somatic components seem to support this hypothesis

Type
Research Papers
Information
Journal of Helminthology , Volume 69 , Issue 4 , December 1995 , pp. 319 - 324
Copyright
Copyright © Cambridge University Press 1995

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