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Biochemical characterization of Taenia hydatigena cysticerci from goats and pigs

Published online by Cambridge University Press:  05 June 2009

S. M. A. Abidi ,
W. A. Nizami ,
P. Khan ,
M. Ahmad  and
M. Irshadullah
S. M. A. Abidi
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh—202 002, India
W. A. Nizami*
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh—202 002, India
P. Khan
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh—202 002, India
M. Ahmad
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh—202 002, India
M. Irshadullah
Affiliation:
Section of Parasitology, Department of Zoology, Aligarh Muslim University, Aligarh—202 002, India
*
*To whom all correspondence should be addressed.
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Abstract

Analysis of the major biochemical components of Taenia hydatigena cysticerci collected from goats and pigs showed marked differences, particularly in glycogen, protein, lipid and DNA levels. Differences were also detected in the levels of cholesterol, triglycerides, free fatty acids and phospholipids. Furthermore, the profile of phospholipid fractions revealed quantitative differences between the two species. It is concluded that the cysticerci of goat and pig origin probably represent two different strains and possibly follow the same pattern of speciation as reported in the related taeniid, Echinococcus granulosus.

Keywords

Taenia hydatigenacysticercigoatpigbiochemistrylipidsglycogenproteinsnucleic acids
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 4 , December 1989 , pp. 333 - 337
Copyright
Copyright © Cambridge University Press 1989

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References

REFERENCES

Arme, C. & Pappas, P. W. (1983) Biology of Eucestoda Volume–II. Academic Press: New York.Google Scholar
Barrett, J. (1981) Biochemistry of Parasitic Helminths. Macmillan: London.CrossRefGoogle Scholar
Bolla., R. I. & Roberts, L. S. (1971) Developmental physiology of cestodes, X. The effect of crowding on carbohydrate levels and on RNA, DNA and protein synthesis in Hymenolepis diminuta. Comparative Biochemistry and Physiology, 40A, 777–787.Google Scholar
Cohen, S. & Warren, K. S. (1982) Immunology of Parasitic Infections. Blackwell Scientific Publications: Oxford.Google Scholar
Dische, Z. (1935) Colour reactions of nucleic acid components. In: The Nucleic Acids. (Editors, Chargaff, E. & Davidson, J. M.) Vol. I, pp. 285–305, Academic Press: New York.Google Scholar
Flisser, A., Willms, K., Laclette, J. P., Larralde, C., Ridaura, C. & Beltran, F. (1982) Cysticercosis: Present State of knowledge and Perspectives. Academic Press: New York.Google Scholar
Folch, J., Lees, M. & Sloane Stanley, G. H. (1957) A simple method for the isolation and purification of total lipids from animal tissue. Journal of Biological Chemistry, 226, 497–509.CrossRefGoogle Scholar
Frayha, G. J. (1968) A study on the synthesis and absorption of cholesterol in hydatid cysts (Echinococcus granulosus). Comparative Biochemistry and Physiology, 27, 875–878.CrossRefGoogle Scholar
Frayha, G. J. (1971) Comparative metabolism of acetate in the taenid tapeworms Echinococcus granulosus, E. multilocularis and Taenia hydatigena. Comparative Biochemistry and Physiology, 39, 167–170.Google Scholar
Giles, K. & Myers, A. (1965) An improved diphenylamine method for the estimation of deoxyribonucleic acid. Nature, 206, 93.Google Scholar
Ginger, C. D. & Fairbairn, D. (1966) Lipid metabolism in helminth parasites. II. The major origins of the lipids of Hymenolepis diminuta (Cestoda). Journal of Parasitology, 52, 1097–1107.CrossRefGoogle ScholarPubMed
Kassis, A. I. & Frayha, G. J. (1973) Lipids of the cysticerci of Taenia hydatigena (Cestoda). Comparative Biochemistry and Physiology, 46B, 435–443.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.Google Scholar
Lowry, R. R. & Tinsley, T. J. (1976) Rapid colorimetric determination of free fatty acids. Journal of the American Oil Chemists' Society, 53, 235–250.Google Scholar
Mcmanus, D. P. (1981) A biochemical study of adult and cystic stage of Echinococcus granulosus of human and animal origin from Kenya. Journal of Helminthology, 55, 21–27.CrossRefGoogle ScholarPubMed
Mcmanus, D. P. & Smyth, J. D. (1978) Differences in the chemical composition and carbohydrate metabolism of Echinococcus granulosus (horse and sheep strains) and E. multilocularis. Parasitology, 77, 103–109.CrossRefGoogle ScholarPubMed
Misra, U. K. (1968) Liver lipids of rats administered excessive amounts of retinol. Canadian Journal of Biochemistry, 46, 697–701.CrossRefGoogle ScholarPubMed
Reid, W. M. (1942) Certain nutritional requirements of the fowl cestode Raillietina cesticillus (Molin) as demonstrated by the short periods of starvation of the host. Journal of Parasitology, 28, 319–340.CrossRefGoogle Scholar
Rishi, A. K. & Mcmanus, D. P. (1988) Molecular cloning of Taenia solium genomic DNA and characterization of taeniid cestodes by DNA analysis. Parasitology, 97, 161–176.CrossRefGoogle ScholarPubMed
Roe, J. H. & Dailey, R. E. (1966) Determination of glycogen with the anthrone reagent. Analytical Biochemistry, 15, 245–250.CrossRefGoogle ScholarPubMed
Rouser, G., Fleischer, S. & Yamamoto, A. (1970) Two-dimensional thin layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids, 5, 494–496.Google Scholar
Sackett, G. E. (1925) Modification of Bloor's method for the determination of cholesterol in whole blood or blood serum. Journal of Biological Chemistry, 64, 203–205.Google Scholar
Skipski, V. P., Peterson, R. F. & Barclay, M. (1964) Quantitative analysis of phospholipids by thin layer chromatography. Biochemical Journal, 90, 374–378.CrossRefGoogle ScholarPubMed
Sokal, R. R. & Rohlf, F. J. (1981) Biometry: The Principles and Practice of Statistics in Biological Research (2nd Edition). W. H. Freeman and Company: San Francisco.Google Scholar
Van handel, E. & Zilversmit, D. B. (1957) Micromethod for the direct determination of triglycerides in serum. Journal of Laboratory and Clinical Medicine, 50, 152–157.Google Scholar
Varma, T. K. & Ahluwalia, S. S. (1986) Some observations on the prevalence and variations in the morphology and biology of Cysticercus tenuicollis of sheep, goat, pig and buffalo origin. Indian Journal of Animal Sciences, 56, 1135–1140.Google Scholar
Varma, T. K. & Ahluwalia, S. S. (1987) Some observations on the development of Taenia hydatigena in pups and laboratory animals. Indian Journal of Animal Sciences, 57, 804–811.Google Scholar
Varma, T. K. & Rao, B. V. (1973) Certain epidemiological studies on larval and strobilar phases of the common dog tapeworm Taenia hydatigena. Indian Journal of Animal Sciences, 43, 534–539.Google Scholar
Von brand, T. (1973) Biochemistry of Parasites. Second Edition. Academic Press: New York.Google Scholar
Yap, K. W., Thompson, R. C. A., Rood, J. I. & Pawlowski, I. D. (1987) Taenia hydatigena: Isolation of mitochondrial DNA, molecular cloning and physical mitochondrial genome mapping. Experimental Parasitology, 63, 288–294.Google Scholar
Zollner, N. & Krisch, K. (1962) Uber die quantitative Bestimmung von Lipoiden (Mikromethode) mittels der vielen natürlichen lipoiden (allen bekannten Plasma-lipoiden) gemeinsamen sulphophosphovanillin Reacktion. Zeitschrift für die Gesamte Experimentelle Medizin, 135, 545–561.Google Scholar