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Echinococcus multilocularis: Identification of proteins inducing antibody formation in metacestode infection

Published online by Cambridge University Press:  18 November 2009

K. Lingelbach  and
E. Hinz
K. Lingelbach
Affiliation:
Institutfur Tropenhygien, Im Neuenheimer Feld 324, 69 Heidelberg, West Germany
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Abstract

An approach to the identification of parasite proteins which are immunogenic in natural infections is described, using the infection with the larval stage of Echinococcus multilocularis as a parasite model. Metacestode proteins were separated by SDS-polyacrylamide gel electrophoresis, and transferred electrophoretically to nitrocellulose sheets (Western blotting). Subsequently, immune recognition of the proteins was performed with various host sera and antigen-antibody complexes were detected enzymatically. Using homologous antisera, different patterns of immunogenic bands were revealed by sera of different host species. Cross-reactions with sera from individuals infected with unrelated helminths were analysed. Four proteins of E. multilocularis which failed to show any cross-reaction were identified.

Type
Research Article
Information
Journal of Helminthology , Volume 58 , Issue 1 , March 1984 , pp. 1 - 6
Copyright
Copyright © Cambridge University Press 1984

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