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Effects of adoptive transfer of immune spleen cells on worm growth and microfilaraemia in Brugia pahangi infection in Mongolian gerbils

Published online by Cambridge University Press:  05 June 2009

A.I. Khan ,
Y. Horii ,
N. Ishikawa  and
Y. Nawa
A.I. Khan
Affiliation:
Department of Parasitology, Miyazaki Medical College, Kiyotake, Miyazaki 889-16, Japan
Y. Horii
Affiliation:
Department of Veterinary Internal Medicine, Faculty of Agriculture, Miyazaki University, Gakuen-Kibanadai, Miyazaki 889-21, Japan
N. Ishikawa
Affiliation:
Department of Parasitology, Miyazaki Medical College, Kiyotake, Miyazaki 889-16, Japan
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Abstract

Protective immunity against Brugia pahangi was examined after adoptive transfer of immune spleen cells. Spleen cells obtained from gerbils at 8 weeks post-infection (p.i.) with 100 infective larvae (L3) of B. pahangi were transferred into naive recipients, and then 24 h later, they were infected with 100 L3 of B. pahangi. The recipients given normal spleen cells and infected by the same manner served as controls. Microfilarial counts in the circulation were monitored at designated times after infection and worm burden and the size of individual female worm were determined at 16 weeks p.i. to evaluate the effects of adoptive immunization. In addition, eosinophil responses and serum antibody titres were examined during the course of infection. In the control group, microfilariae first appeared in circulation at 9 weeks p.i. and continuously increased in number throughout the course examined. In contrast, microfilaraemia was almost completely suppressed in the group given immune spleen cells. Although worm burden was comparable between the two groups, the average size of female adult worms recovered from the adoptively immunized group was significantlysmaller than that from the control group. Eosinophil response was hastened and enhanced by adoptive transfer of immune spleen cells in the early stage of infection. Parasite- specific antibody response was also hastened by adoptive immunization. These results suggest that immune spleen cells could confer protective immunity mainly directed against adult B. pahangi.

Type
Research Papers
Information
Journal of Helminthology , Volume 69 , Issue 4 , December 1995 , pp. 331 - 335
Copyright
Copyright © Cambridge University Press 1995

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