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Efficacy of UV-irradiated larval vaccine of Ancylostoma ceylanicum (Looss, 1911) in golden hamsters (Mesocricetus auratus)

Published online by Cambridge University Press:  05 June 2009

Sushila Menon  and
M. K. Bhopale
Sushila Menon
Affiliation:
Department of Zoonosis, Haffkine Institute, Parel, Bombay-12, India
M. K. Bhopale
Affiliation:
Department of Zoonosis, Haffkine Institute, Parel, Bombay-12, India
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Abstract

A vaccination trial in golden hamsters with UV-irradiated infective larvae of Ancylostoma ceylanicum was attempted. One oral vaccination of hamsters with 100 infective larvae irradiated by means of UV-tube (390 nm) at different time intervals induced the development of resistance. As the time exposure of irradiation was increased, there was a corresponding decrease in the subsequent worm establishment. A high level of protection afforded by larvae irradiated for 15min UV-exposure was recorded giving 99·0% and 95·0% worm reduction against the challenge doses of 100 and 1000 normal larvae respectively. There was no marked difference in worm establishment in hamsters vaccinated either orally or subcutaneously, followed by oral challenge. In the vaccinated hamsters, the manifestations of resistance at 15 min UV-exposure were shown by marked reduction in worm establishment and highly reduced epg in pellets with significantly higher blood haemoglobin levels compared with those given normal larvae as vaccine and challenge controls.

Type
Research Article
Information
Journal of Helminthology , Volume 59 , Issue 4 , December 1985 , pp. 287 - 293
Copyright
Copyright © Cambridge University Press 1985

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