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Efficacy of UV-irradiated larval vaccine of Ancylostoma ceylanicum (Looss, 1911) in golden hamsters (Mesocricetus auratus)

Published online by Cambridge University Press:  05 June 2009

Sushila Menon  and
M. K. Bhopale
Sushila Menon
Affiliation:
Department of Zoonosis, Haffkine Institute, Parel, Bombay-12, India
M. K. Bhopale
Affiliation:
Department of Zoonosis, Haffkine Institute, Parel, Bombay-12, India
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Abstract

A vaccination trial in golden hamsters with UV-irradiated infective larvae of Ancylostoma ceylanicum was attempted. One oral vaccination of hamsters with 100 infective larvae irradiated by means of UV-tube (390 nm) at different time intervals induced the development of resistance. As the time exposure of irradiation was increased, there was a corresponding decrease in the subsequent worm establishment. A high level of protection afforded by larvae irradiated for 15min UV-exposure was recorded giving 99·0% and 95·0% worm reduction against the challenge doses of 100 and 1000 normal larvae respectively. There was no marked difference in worm establishment in hamsters vaccinated either orally or subcutaneously, followed by oral challenge. In the vaccinated hamsters, the manifestations of resistance at 15 min UV-exposure were shown by marked reduction in worm establishment and highly reduced epg in pellets with significantly higher blood haemoglobin levels compared with those given normal larvae as vaccine and challenge controls.

Type
Research Article
Information
Journal of Helminthology , Volume 59 , Issue 4 , December 1985 , pp. 287 - 293
Copyright
Copyright © Cambridge University Press 1985

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References

REFERENCES

Bezubik, B. (1972) Immunological response of 1-month old lambs to normal and UV-irradiated larvae of Strongyloides papillosus. Acta Parasitologica Polonica, 20, 587–595.Google Scholar
Bezubik, B., Jeska, E. L. & Wedrychowicz, H. (1977) Immunological response of sheep to UVirradiated larvae of Ostertagia circumcincta. Acta Parasitologica Polonica, 24, 323–333.Google Scholar
Dow, C., Jarrett, W. F. H., Jennings, F. W., Mcintyre, W. I. M. & Mulligan, W. (1959) The production of active immunity against hookworm Uncinaria stenocephala. Journal of the American Veterinary Medical Association, 135, 407–411.Google ScholarPubMed
Dow, C.Jarrett, W. F. H., Jennings, F. W., Mcintyre, W. I. M. & Mulligan, W. (1961) Studies on immunity to Uncinaria stenocephala infection in the dog: double vaccination with irradiated larvae. American Journal of Veterinary Research, 22, 352–354.Google ScholarPubMed
Foster, A. C. (1935) The immunity of dogs to Ancylostoma caninum. American Journal of Hygiene, 22, 65–105.Google Scholar
Herrick, C. A. (1928) A quantitative study of infections with Ancylostoma caninum in dogs. American Journal of Hygiene, 8, 125–157.Google Scholar
Jarrett, W. F. H., Jennings, F. W., Mcintyre, W. I. M., Mulligan, W., Sharp, N. C. C. & Urquhart, G. M. (1959) Immunological studies on Dictyocaulus viviparus infections in calves: double vaccination with irradiated larvae. American Journal of Veterinary Research, 20, 525–526.Google Scholar
Jarrett, W. F. H., Jennings, F. W., Mcintyre, W. I. M., Mulligan, W. & Urquhart, G. M. (1960) Immunological studies on Dictyocaulus viviparus infection: Active immunization with whole serum vaccine. Immunology, 3, 135–144.Google Scholar
Jennings, F. W., Mulligan, W. & Urquhart, G. M. (1963) Variables in X-ray inactivation of Nippostrongylus brasiliensis larvae. Experimental Parasitology, 13, 367–372.CrossRefGoogle ScholarPubMed
Katiyar, J. C.Mukerji, A. & Sen, A. B. (1968) Immunogenic effect of ultraviolet treated larvae of Nippostrongylus brasiliensis in rats. Indian Journal of Experimental Biology, 6, 35–37.Google ScholarPubMed
Keeling, J. E. D. (1960) The effects of ultraviolet radiation on Nippostrongylus muris. 1. Irradiation of infective larvae: lethal and sublethal effects. Annals of Tropical Medicine and Parasitology, 54,182–191.CrossRefGoogle Scholar
Mccoy, O. R. (1931) Immunity reactions of the dog against hookworm Ancylostoma caninum under conditions of repeated infection. American Journal of Hygiene, 14, 268–303.Google Scholar
Miller, T. A. (1964) Effect of X-irradiation upon the infective larvae of Ancylostoma caninum and the immunogenic effect in dogs of a single infection with 40 Kr. irradiated larvae. Journal of Parasitology, 50, 735–742.CrossRefGoogle ScholarPubMed
Miller, T. A. (1965) Studies in canine ancylostomiasis: double vaccination with X-irradiated Ancylostoma caninum larvae. Journal of American Veterinary Medical Association, 146, 41–44.Google ScholarPubMed
Miller, T. A. (1966) Comparison of the immunogenic efficiencies of normal and X-irradiated Ancylostoma caninum larvae in dogs. Journal of Parasitology, 52, 512–519.CrossRefGoogle ScholarPubMed
Miller, T. A. (1971) Vaccination against the canine hookworm diseases. Advances in Parasitology, 9, 153–183.CrossRefGoogle ScholarPubMed
Otto, G. F. & KERR, K. B. (1939) Immunization of dogs against hookworm, Ancylostoma caninum by subcutaneous injection of graded doses of living larvae. American Journal of Hygiene, 29, 25–45.Google Scholar
Ray, D. K. & Bhopale, K. K. (1972) Complete development of Ancylostoma ceylanicum (Looss, 1911) in golden hamsters Mesocricetus auratus. Experientia, 28, 359.CrossRefGoogle ScholarPubMed
Ray, D. K., Bhopale, K. K. & Shrivastava, V. B. (1972) Migration and growth of Ancylostoma ceylanicum in golden hamsters Mesocricetus auratus. Journal of Helminthology, 46, 357–362.CrossRefGoogle ScholarPubMed
Sarles, M. P. (1929) The length of life and rate of loss of the dog hookworm, Ancylostoma caninum. American Journal of Hygiene, 10, 667–681Google Scholar
Stowens, D. (1942) The effect of ultraviolet irradiation on Trichinella spiralis. American Journal of Hygiene, 36, 264–268.Google Scholar
Vieira, R. A. & Rombert, P. C. (1974) Contribution to the study of hookworm immunity. 3. New findings on the immunization of dogs with UV-attenuated Ancylostoma caninum larvae. Anais do Instituto do Higiene e Medicina Tropical, 2, 485–499.Google Scholar
VinaYak, V. K., Gupta, N. K., Chopra, A. K., Sharma, G. L. & Kumar, Asha (1981) Efficacies of vaccines against canine hookworm disease. Parasitology, 82, 375–382.CrossRefGoogle ScholarPubMed