Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-01T21:14:18.926Z Has data issue: false hasContentIssue false
  • English
  • Français

Enzyme polymorphism in Schistosoma mattheei from cattle in the Eastern Transvaal Lowveld

Published online by Cambridge University Press:  05 June 2009

F. J. Kruger
F. J. Kruger
Affiliation:
Research Institute for Diseases in a Tropical Environment of the South African Medical Research Council, P.O. Box 634, Nelspruit 1200, Republic of South Africa
Get access Rights & Permissions [Opens in a new window]

Abstract

Enzyme electrophoresis was conducted on 10 Schistosoma mattheei adult worm samples, comprising 270 individuals, collected from cattle in the Eastern Transvaal Lowveld. Glucose-6-phosphate dehydrogenase (G6PDH) was studied in all the samples and phosphoglucomutase (PGM) and malate dehydrogenase (MDH) in five populations each. Only one population was polymorphic for G6PDH. In this population, in addition to the allele found in all the other samples, a second allele occurred with a similar Rf value to S. haematobium. The two alleles were in Hardy-Weinberg equilibrium. MDH-1 exhibited two alleles. However, these alleles were not in equilibrium. In certain populations, heterozygotes occurred together with homozygotes of one of the alleles only. PGM was monomorphic in all the populations studied.

Keywords

Schistosoma mattheeiSchistosoma haematobiumcattleenzyme electrophoresis
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 3 , September 1989 , pp. 191 - 196
Copyright
Copyright © Cambridge University Press 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Fletcher, M., Loverde, P. T. & Woodruff, D. S. (1981) Genetic variation in Schistosoma mansoni: enzyme polymorphisms in populations from Africa, Southwest Asia, South America, and the West Indies. American Journal of Tropical Medicine and Hygiene, 30, 406–421.CrossRefGoogle ScholarPubMed
Fletcher, M., Woodruff, D. S. & Loverde, P. T. (1980) Genetic differentation between Schistosoma mekongi and S. japonicum: an electrophoretic study. In: The Mekong Schistosome (editors, Bruce, J. I. & Sornmani, S.) pp. 113–122. Malacological Review: Supplement 2.Google Scholar
Kruger, F. J. (1987) Enzyme electrophoresis of South African Schistosoma mattheei and S. haematobium. Onderstepoort Journal of Veterinary Research, 53, 109–110.Google Scholar
Kruger, F. J. (1988) Further observations on the electrophoretic characterization of South African Schistosoma mattheei and S. haematobium. Onderstepoort Journal of Veterinary Research, 55, 67–68.Google ScholarPubMed
Loverde, P. T., Dewald, J. & Minchella, D. J. (1985) Further studies of genetic variation in Schistosoma mansoni. Journal of Parasitology, 71, 732–734.CrossRefGoogle ScholarPubMed
Pitchford, R. J. (1959) Cattle schistosomiasis in man in the Eastern Transvaal. Transactions of the Royal Society of Tropical Medicine and Hygiene, 53, 285–290.CrossRefGoogle ScholarPubMed
Pitchford, R. J. (1961) Observations on a possible hybrid between the two schistosomes S. haematobium and S. mattheei. Transactions of the Royal Society of Tropical Medicine and Hygiene, 55, 44–51.CrossRefGoogle ScholarPubMed
Ross, G. C., Southgate, V. R. & Knowles, R. J. (1978) Observations on some isoenzymes of strains of Schistosoma bovis, S. mattheei, S. margrebowiei, and S. leiperi. Zeitschrift für Parasitenkunde, 57, 49–56.CrossRefGoogle Scholar
Woodruff, D. S., Merenlender, A. M., Upatham, E. S. & Viyanant, V. (1987) Genetic variation and differentiation of three Schistosoma species from the Philippines, Laos, and Peninsular Malaysia. American Journal of Tropical Medicine and Hygiene, 36, 345–354.CrossRefGoogle ScholarPubMed
Wright, C. A. & Ross, G. C. (1980) Hybrids between Schistosoma haematobium and S. mattheei and their identification by isoelectric focusing. Transactions of the Royal Society of Tropical Medicine and Hygiene, 74, 326–332.CrossRefGoogle ScholarPubMed
Wright, C. A. & Ross, G. C. (1983) Enzyme analysis of Schistosoma haematobium. Bulletin of the World Health Organization, 61, 307–316.Google ScholarPubMed