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Evaluation of the therapeutic effect of Olibanum extract against enteric and intramuscular phases of trichinosis in experimentally infected mice

Published online by Cambridge University Press:  26 May 2023

A.M. Matar Open the ORCID record for A.M. Matar [Opens in a new window] ,
M.A. Kora  and
S.S. Shendi
A.M. Matar*
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, Menoufia University, Shebin al-Kom, Menoufia, 6132720, Egypt
M.A. Kora
Affiliation:
Department of Pathology, Faculty of Medicine, Menoufia University, Shebin al-Kom, Menoufia, 6132720, Egypt
S.S. Shendi
Affiliation:
Department of Clinical and Molecular Parasitology, National Liver Institute, Menoufia University, Shebin al-Kom, Menoufia, 6132720, Egypt
*
Corresponding author: Amira M. Matar; Email: amira.matar@med.menofia.edu.eg
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Abstract

Trichinosis is a global food-borne zoonotic disease. Most drugs used in its treatment have low bioavailability and reduced activity against larvae. Therefore, there is an urgent need for safe and effective medications. This study aimed to investigate the in vivo anti-parasitic and anti-inflammatory efficacy of olibanum (OL) extract, alone or combined with albendazole (ABZ) during both intestinal and muscular phases of trichinosis. Male Swiss albino mice (n = 130) were allocated to seven groups, with 20 mice in each group except for the negative control group (10 mice): negative control (GI), positive control (GII), OL25- treated (GIII), OL50- treated (GIV), ABZ50- treated (GV), OL25 + ABZ25 (GVI), and OL50 + ABZ25 (GVII). For intestinal and muscular phase analysis, each group was divided into two subgroups based on euthanizing day (6 and 35 days post-infection). The drug’s efficacy was evaluated through parasitological, biochemical, histopathological, and immunohistochemical studies. OL extract at both concentrations (25 mg/kg/d, 50 mg/kg/d) significantly reduced adult (53.7% and 68.1%, respectively) and larval counts (57.3% and 78.8%, respectively). It improved the histopathological changes in intestine and muscle. The expression of CD8+ T cells and the serum level of IL-10 increased significantly during both intestinal and muscular phases (P < 0.05) in OL50 treated mice. Additionally, OL decreased abnormal levels of liver enzymes (ALT & AST). Its effects were dose-dependent in both adult and larval stages. In conclusion, OL exhibits promising in vivo activity against both stages of Trichinella spiralis infection, particularly at the intramuscular phase. It can be safe as an alternative treatment for trichinosis.

Keywords

T. spiralisolibanumalbendazoleIL-10CD8
Type
Research Paper
Information
Journal of Helminthology , Volume 97 , 2023 , e44
Copyright
© The Author(s), 2023. Published by Cambridge University Press

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