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Factors affecting the acquisition of resistance against Schistosoma mansoni in the mouse. I. Demonstration of resistance to reinfection using a model system that involves perfusion of mice within three weeks of challenge

Published online by Cambridge University Press:  05 June 2009

M. Doenhoff ,
Q. Bickle ,
E. Long ,
J. Bain  and
A. McGregor
M. Doenhoff
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, St. Albans AL4 0XQ, England
Q. Bickle
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, St. Albans AL4 0XQ, England
E. Long
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, St. Albans AL4 0XQ, England
J. Bain
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, St. Albans AL4 0XQ, England
A. McGregor
Affiliation:
Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, St. Albans AL4 0XQ, England
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Abstract

The degree of resistance acquired by Schistosoma mansoni-infected mice against homologous challenge has been determined by perfusion of the animals within three weeks of the challenge, at which time the challenge-derived organisms were morphologically distinguishable from the primary infection which induced the resistance. The method has been compared with assays based on determination of the number of organisms migrating through the lung, and with perfusions at a later time when the challenge has matured. The results obtained with the three week perfusion method, showing that resistance was acquired by eight weeks after a primary infection, were confirmed by the longer survival of, and reduced egg excretion rates and tissue egg burdens in the experimental animals relative to respective challenge control animals. However, some discrepancy in challenge-derived worm numbers was found between animals perfused three weeks after challenge and those autopsied at later times. The possible reasons for this difference are discussed. The degree of resistance that was acquired was to some extent dependent on the size of the challenge infection.

Type
Research Papers
Information
Journal of Helminthology , Volume 52 , Issue 3 , September 1978 , pp. 173 - 186
Copyright
Copyright © Cambridge University Press 1978

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References

REFERENCES

Bell, D. R. (1963) A new method for counting Schistosoma mansoni eggs in faeces. With special reference to therapeutic trials. Bulletin of the World Health Organization, 29, 525–530.Google ScholarPubMed
Cheever, A. W. (1968) Conditions affecting the accuracy of potassium hydroxide digestion techniques for counting Schistosoma mansoni eggs in tissue. Bulletin of the World Health Organization, 39, 328–331.Google Scholar
Clegg, J. A. (1965) In vitro cultivation of Schistosoma mansoni. Experimental Parasitology, 16, 133–147.CrossRefGoogle ScholarPubMed
Ford, C. E., Hamerton, J. L., Barnes, D. W. H. and Loutit, J. F. (1956) Cytological identification of radiation chimaeras. Nature, London, 177, 452–454.CrossRefGoogle ScholarPubMed
Hunter, G. W., Crandall, R. B., Zickafoose, D. E. and Purvis, Q. B. (1962) Studies on schistosomiasis. XVIII. Some factors affecting resistance to Schistosoma mansoni infections in albino mice. American Journal of Tropical Medicine and Hygiene, 11, 17–24.CrossRefGoogle Scholar
James, E. R. and Taylor, M. G. (1976) Transformation of cercariae to schistosomula: A quantitative comparison of transformation techniques and of infectivity by different injection routes of the organisms produced. Journal of Helminthology, 50, 223–233.CrossRefGoogle ScholarPubMed
Knopf, P. M., Nutman, T. B. and Reasoner, J. A. (1977) Schistosoma mansoni: Resistance to reinfection in the rat. Experimental Parasitology, 41, 74–82.CrossRefGoogle ScholarPubMed
Lichtenberg, F.von, Sher, A. and McIntyre, S. (1977) A lung model of schistosome immunity in mice. American Journal of Pathology, 87, 105–120.Google Scholar
Mahmoud, A. A. F., Warren, K. S. and Graham, R. C. (1975) Anti-eosinophil serum and the kinetics of eosinophilia in Schistosomiasis mansoni. Journal of Experimental Medicine, 142, 560–574.CrossRefGoogle Scholar
Olivier, L. (1952) A comparison of infections in mice with three species of schistosomes, Schistosoma mansoni, Schistosoma japonicum and Schistosomatium douthitti. American Journal of Hygiene, 55, 22–35.Google ScholarPubMed
Olivier, L. J. (1966) Infectivity of Schistosoma mansoni cercariae. American Journal of Tropical Medicine and Hygiene, 15, 883–885.Google Scholar
Olivier, L. J. and Schneidermann, J. (1953) Acquired resistance to Schistosoma mansoni infection in laboratory animals. American Journal of Tropical Medicine and Hygiene, 2, 298–306.Google Scholar
Perez, H., Clegg, J. A. and Smithers, S. R. (1974) Acquired immunity to Schistosoma mansoni in the rat: measurement of immunity by the lung recovery technique. Parasitology, 69, 349–359.Google Scholar
Reid, W. A., Phillips, S. M. and Roscinski, R. J. (1977) Schistosoma mansoni: Radiosotope uptake and retention by cercariae and developing schistosomules. Experimental Parasitology, 42, 331–342.CrossRefGoogle Scholar
Sher, A., Mackenzie, P. and Smithers, S. R. (1974) Decreased recovery of invading parasites from the lungs as a parameter of acquired immunity to schistosomiasis in the mouse. Journal of Infectious Diseases, 130, 626–633.CrossRefGoogle ScholarPubMed
Sher, A., Smithers, S. R. and Mackenzie, P. (1975) Passive transfer of acquired resistance to Schistosoma mansoni in laboratory mice. Parasitology, 70, 347–357.CrossRefGoogle ScholarPubMed
Smithers, S. R. and Terry, R. J. (1965) The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology, 55, 695–700.CrossRefGoogle ScholarPubMed
Smithers, S. R. and Terry, R. J. (1969) Immunity in schistosomiasis. Annals of the New York Academy of Science, 160, 826–840.CrossRefGoogle ScholarPubMed
Taylor, M. G., Amin, M. B. A. and Nelson, G. S. (1969) ‘Parthogenesis’ in Schistosoma mattheei. Journal of Helminthology, 43, 197–206.CrossRefGoogle Scholar
Thompson, J. H. (1954) Host-parasite relationships of Schistosoma mansoni. Experimental Parasitology, 3, 140–160.CrossRefGoogle ScholarPubMed