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Genetic diversity among isolates of Trichinella spiralis from the Province of Buenos Aires, Argentina

Published online by Cambridge University Press:  12 April 2024

A. Sequeira*
Affiliation:
Departamento de Parasitología Sanitaria, Instituto Nacional de Parasitología, ANLIS, Dr Carlos G. Malbran, CP 1281, Buenos Aires, Argentina
V. Molina
Affiliation:
Departamento de Parasitología Sanitaria, Instituto Nacional de Parasitología, ANLIS, Dr Carlos G. Malbran, CP 1281, Buenos Aires, Argentina
M. Monfellano
Affiliation:
Departamento de Parasitología Sanitaria, Instituto Nacional de Parasitología, ANLIS, Dr Carlos G. Malbran, CP 1281, Buenos Aires, Argentina
J. Bolpe
Affiliation:
Departamento de Parasitología Sanitaria, Instituto Nacional de Parasitología, ANLIS, Dr Carlos G. Malbran, CP 1281, Buenos Aires, Argentina
E. Guarnera
Affiliation:
Departamento de Parasitología Sanitaria, Instituto Nacional de Parasitología, ANLIS, Dr Carlos G. Malbran, CP 1281, Buenos Aires, Argentina
*
*Present address: Museum of Comparative Zoology Laboratories, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA. Fax: 1 617 495 5667 E-mail: asequeira@oeb.harvard.edu

Abstract

Random Amplified Polymorphic DNAs, (RAPDs) are used to study the occurrence of Trichinella britovi and T5 among domestic animals in the Province of Buenos Aires, Argentina and to assess the genetic diversity among isolates of T. spiralisfrom this area in a number of infected hosts. All the local isolates proved to be T. spiralis. Six of the eight primers used indicate that the Buenos Aires isolates are distinct from each other as they produce a considerable number of polymorphic bands. Our overall estimates are relatively higher than other intraspecific distances previously estimated within species of this genus and among T. spiralis isolates. Such high degrees of variability observed among local isolates and between isolates from Buenos Aires and Spain should be taken into account when defining isolates within this species, and considering differences in the epidemiology of T. spiralis.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2000

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References

Black, W. (1997) Rapds, Rapddist 1.0. Department of Microbiology, Colorado State University, Fort Collins, Colorado.Google Scholar
Bolas-Fernandez, F. & Wakelin, D. (1989) Infectivity of Trichinella isolates in mice is determined by host inmune responsiveness. Parasitology 99, 8388.CrossRefGoogle Scholar
Bolas-Fernandez, F. & Wakelin, D. (1990) Infectivity, antigenicity and host responses to isolates of the genus Trichinella . Parasitology 100, 491497.CrossRefGoogle ScholarPubMed
Capó, V. & Despommier, D. (1996) Clinical aspects of infection with Trichinellaspp. Clinical Microbiological Reviews 96, 4754.CrossRefGoogle Scholar
Cox, F.E.G. (1991) Variation and vaccination. Nature 349, 193.CrossRefGoogle ScholarPubMed
Dame, J.B. & McCutchan, T.F. (1995) Cloning and characterization of ribosomal DNA from Plasmodium berghei . Molecular and Biochemical Parasitology 8, 263279.CrossRefGoogle Scholar
Dame, J.B., Murrell, K.D., Worley, D.E. & Schad, G.A. (1987) Trichinella spiralis: genetic evidence for synanthropic subspecies in sylvatic hosts. Experimental Parasitology 64, 195203.CrossRefGoogle ScholarPubMed
de Meeûs, T., Michalakis, Y. & Renaud, F. (1998) Santa Rosalia revisted: or Why are there so many kinds of parasites in “The Garden of earthly delights”?. Parasitology Today 14, 1013.CrossRefGoogle Scholar
Despommier, D.D. (1983) Biology. pp. 75142 in Campbell, W.C. (Ed.) Trichinella and trichinellosis. New York, Plenum Press.CrossRefGoogle Scholar
Dupouy-Camet, J., Robert, F., Guillou, J.P., Vallet, C., Perret, C. & Soule, C. (1993) Genetic analysis of Trichinellaisolates with random amplified polymorphic DNA markers. in Campbell, W.C., Pozio, E. & Bruschi, F. (Eds) Trichinellosis. Proceedings of the 8th International Conference on Trichinellosis 1993. Rome, Instituto Superiore di Sanitá Press.Google Scholar
Dupouy-Camet, J., Robert, F., Guillou, J.P., Vallet, C., Perret, C. & Soule, C. (1994) Identification of Trichinella isolates with random amplified polymorphic DNA markers. Parasitology Research 80, 358360.CrossRefGoogle ScholarPubMed
Dupouy-Camet, J., Soule, C., Guillou, J.P., Rouer, E., Lavareda, de, Souza, S., Ancelle, T. & Benarous, R. (1991) Detection of repetitive sequences of Trichinella spiralis by the polymerase chain reaction in experimentally infected mice. Parasitology Research 77, 180182.CrossRefGoogle ScholarPubMed
Felsestein, J. (1993) PHYLIP (Phylogeny inference package) v. 3.5c. Department of Genetics, The University of Washington, Seattle, Washington.Google Scholar
Gari-Toussaint, M., Bernard, E., Quaranta, J.F., Marty, P., Soler, C., Ozouf, N. & Caux, C. (1993) First report in France of an outbreak of human trichinellosis due to Trichinella britovi. pp. 465468 in Campbell, W.C., Pozio, E. & Bruschi, F. (Eds) Trichinellosis. Proceedings of the 8th International Conference on Trichinellosis 1993. Rome, Instituto Superiore di Sanitá Press.Google Scholar
Gasser, R.B., Zhu, X.Q., Monti, J.R., Dou, L., Cai, X. & Pozio, E. (1998) PCR-SSCP of rDNA for the identification of trichinella isolated from mainland China. Molecular and Cellular Probes 12, 2734.CrossRefGoogle ScholarPubMed
Gottstein, B., Pozio, E., Connolly, B., Gamble, H.R., Eckert, J. & Jakob, H.P. (1997) Epidemiological investigation of trichinellosis in Switzerland. Veterinary Parasitology 72, 201207.CrossRefGoogle ScholarPubMed
Guo, Z.G., Gross, U. & Johnson, A.M. (1997) Toxoplasma gondii virulence markers identified by random amplified polymorphic DNA polymerase chain reaction. Parasitology Research 83, 458463.CrossRefGoogle ScholarPubMed
Klassen, G.R., Thiessen, J.L. & Dick, T.A. (1986) Strain specific 1.7 kilobase repetitive deoxyribonucleic acid sequence family in Trichinella spiralis . Molecular and Biochemical Parasitology 21, 227233.CrossRefGoogle ScholarPubMed
MacLean, J.D., Viallet, J., Law, C. & Staudt, M. (1989) Trichinosis in the Canadian Arctic: report of five outbreaks and a new clinical syndrome. Journal of Infectious Diseases 160, 513520.CrossRefGoogle Scholar
McManus, D.P. & Bowles, J. (1996) Molecular genetic approaches to parasite identification: their value in diagnostic parasitology and systematics. International Journal for Parasitology 26, 687704.CrossRefGoogle ScholarPubMed
Minchella, D.J., Barnstetter, B.A. & Kazacos, K.R. (1989) Molecular characterization of sylvatic isolates of Trichinella spiralis . Journal of Parasitology 75, 388392.CrossRefGoogle ScholarPubMed
Montali, J.G. (1997) Dirección Provincial de Ganadería. Ministerio de Asuntos Agrarios. Informe de la Primera Reunión Nacional de Triquinosis, Buenos Aires, Argentina.Google Scholar
Murrell, K.D. & Bruschi, F. (1994) Clinical trichinellosis. pp. 117150 in Sun, T. (Ed.) Progress in clinical parasitology. Boca Ratón, Florida, CRC Press.Google Scholar
Mydynski, L.J. & Dick, T.A. (1985) The use of enzyme polymorphisms to identify genetic differences in the genus Trichinella . Journal of Parasitology 71, 671677.CrossRefGoogle ScholarPubMed
Nei, M. (1972) Genetic distance between populations. American Naturalist 106, 283292.CrossRefGoogle Scholar
Nei, M. & Li, W.H. (1985) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences, USA 76, 5296–5273.Google Scholar
Pozio, E. (1996) Experimental and human influence on the ecology of Trichinella spiralis and Trichinella britovi in Western Europe. Parasitology 113, 527533.CrossRefGoogle Scholar
Pozio, E. (1998) Trichinellosis in the European Union. Epidemiology, ecology and economic impact. Parasitology Today 14, 3538.CrossRefGoogle ScholarPubMed
Pozio, E., La, Rosa, G., Murrell, K.D. & Lichtenfels, J.R. (1992) Taxonomic revisions of the genus Trichinella . Journal of Parasitology 78, 654659.CrossRefGoogle ScholarPubMed
Pozio, E., La, Rosa, G., Rossi, P.V. & Murrell, K.D. (1992) Biological characterization of Trichinella isolates from various host species and geographical regions. Journal of Parasitology 78, 647653.CrossRefGoogle ScholarPubMed
Pozio, E., Varese, P., Morales, M.A., Croppoi, G.P., Pellucia, D. & Bruschi, F. (1993) Comparison of human trichinellosis caused by T. spiralis and T. britovi . American Journal of Tropical Medicine and Hygiene 48, 568575.CrossRefGoogle Scholar
Renault, F., Clayton, D. & de, Meeûs T. (1996) Biodiversity and evolution in host-parasite associations. Biodiversity and Conservation 5, 963974.Google Scholar
Rodriguez, E., Nieto, J., Castillo, J.A. & Garate, T. (1996) Characterization of Spanish Trichinellaisolates by random amplified polymorphic DNA (RAPD). Journal of Helminthology 70, 335343.CrossRefGoogle ScholarPubMed
Sneath, P.H.A. & Sokal, R.R. (1973) Numerical taxonomy: the principles and practice of numerical classification. San Francisco, Freeman.Google Scholar
Timm, R.M & Clauson, B.L. (1987) Coevolution. McGraw-Hill.Google Scholar
Wakelin, D. & Denham, D.A. (1983) The inmune response. pp. 265308 in Campbell, W.C. (Ed.) Trichinella and trichinellosis. New York, Plenum Press.CrossRefGoogle Scholar
Wakelin, D. & Goyal, P.K. (1996) Trichinellaisolates: parasite variability and host responses. International Journal for Parasitology 26, 471481.CrossRefGoogle ScholarPubMed
Wang, H., Zhang, Y., Lao, W. & Wu, Z. (1995) Restriction fragment length polymorphism (RFLP) analysis of genomic DNA of five strains of Trichinella spiralis in China. Chinese Medical Sciences Journal 10, 131135.Google ScholarPubMed
Wu, Z., Nagano, I. & Takahashi, Y. (1998) The detection of Trichinella with polymerase chain reaction (PCR) primers constructed using sequences of random amplified polymorphic DNA (RAPD) or sequences of complementary DNA encoding excretory-secretory (E-S) glycoproteins. Parasitology 117, 173183.CrossRefGoogle Scholar
Zarlenga, D.S, Al-Yaman, F., Minchella, D. & La Rosa, S. (1991) A repetitive DNA probe specific for a North American sylvatic genotype in Trichinella . Molecular and Biochemical Parasitology 48, 131138.CrossRefGoogle ScholarPubMed