Hostname: page-component-77c89778f8-cnmwb Total loading time: 0 Render date: 2024-07-16T17:31:49.888Z Has data issue: false hasContentIssue false
  • English
  • Français

Genomic variability within laboratory and wild subspecies of Heligmosomoides polygyrus

Published online by Cambridge University Press:  05 June 2009

M.A. Abu-Madi ,
A.P. Reid ,
J.W. Lewis  and
W.M. Hominick
M.A. Abu-Madi
Affiliation:
Department of Biology, Royal Holloway, University of London, Egham, Surrey, TW20 OEX, UK
A.P. Reid
Affiliation:
Imperial College of Science, Technology and Medicine, Department of Biology, Silwood Park, Ascot, Berks, SL5 7PY, UK
J.W. Lewis
Affiliation:
Department of Biology, Royal Holloway, University of London, Egham, Surrey, TW20 OEX, UK
W.M. Hominick
Affiliation:
Imperial College of Science, Technology and Medicine, Department of Biology, Silwood Park, Ascot, Berks, SL5 7PY, UK
Get access Rights & Permissions [Opens in a new window]

Abstract

Genomic DNA extracted from laboratory and wild subspecies of the trichostrongyle nematode Heligmosomoides polygyrus were compared using RFLP and DNA/DNA hybridization techniques. Eight restriction endonuclease digests of the genomic DNA of the two subspecies were hybridized with heterologous ribosomal DNA probes and the total radio-isotope labelled DNA of the laboratory subspecies. DNA hybridization of the two subspecies of H. polygyrus yielded different banding patterns when probed with the rDNA clones in Pvu II digests and when total genomic DNA was used as the probe in Hind III and Pvu II digests. The remaining hybridization profiles of both subspecies were identical.

Type
Research Papers
Information
Journal of Helminthology , Volume 68 , Issue 2 , June 1994 , pp. 93 - 96
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abad, P., Tares, S., Brugier, N. & De Guiran, G. (1991) Characterization of the relationships in the pinewood nematode species complex (PWNSC) (Bursaphelenchus spp.) using a heterologous unc-22 DNA probe from Caenorhabditis elegans. Parasitology 102, 303–308.CrossRefGoogle Scholar
Abu-Madi, M.A., Pleass, R.J. & Lewis, J.W. (1994) Metabolic labelling of wild and laboratory subspecies of the trichostrongyle nematode Heligmosomoides polygyrus. Veterinary Parasitology in press.CrossRefGoogle ScholarPubMed
Bartleft, A. & Ball, P.A.J. (1972) Nematospiroides dubius in the mouse as a possible model of endemic human hookworm infection. Annals of Tropical Medicine and Parasitology 66, 129–134.CrossRefGoogle Scholar
Behnke, J.M. (1987) Evasion of immunity by nematode parasites causing chronic infections. Advances in Parasitology 26, 1–17.CrossRefGoogle ScholarPubMed
Behnke, J.M. (1990) Parasites: immunity and pathology. London, Academic Press.Google Scholar
Behnke, J.M., Keymer, A.E. & Lewis, J.W. (1991) Heligmosomoides polygyrus or Nematospiroides dubius? Parasitology Today 7, 177–179.CrossRefGoogle ScholarPubMed
Behnke, J.M. & Wakelin, D. (1977) Nematospiroides dubius: the stimulation of acquired immunity in inbred strains of mice. Journal of Helminthology 51, 167–176.CrossRefGoogle ScholarPubMed
Cameron, M.L., Levy, P., Nutman, T., Vanamala, C.R., Narayanan, P.R. & Rajan, T.V. (1988) Use of restriction fragment length polymorphisms (RFLPs) to distinguish between nematodes of pathogenic significance. Parasitology 96, 381–390.Google Scholar
Chambers, A.E., Almond, N.M., Simpson, A.J.G. & Parkhouse, R.M.E. (1986) Repetitive DNA as a tool for the identification and comparison of nematode variants: application to Trichinella isolates. Molecular and Biochemical Parasitology 21, 113–120.Google Scholar
Denhardt, D.T. (1966). A membrane-filter technique for the detection of complimentary DNA. Biochemical and Biophysical Research Communications 23, 6421–6426.Google Scholar
Dick, T.A., Currans, J. & Klassen, G. (1985) Genetics and molecular biology of Trichinella in ICT6. pp. 118–128in Kim, W.K. (Ed.) Trichinellosis. New York, State University of New York Press.Google Scholar
Durette-Desset, M-C. (1968a) Identification des strongles des mulots et campagnols decrits par Dujardin. Annales de Parasitologie (Paris) 43, 387–404.Google Scholar
Durette-Desset, M-C. (1968b) Les systemes des cretes cuticulaires chez les nematodes heligmosomes. III. Etude de sept especes parasites de rongeurs nearctiques et retablissement du genre Heligmosomoides Hall, 1916. Bulletin du Museum Nationale d'Histoire Naturelle 40, 186–209.Google Scholar
Durefte-Desset, M-C., Kinsella, J.M. & Forrester, D.J. (1972) Arguments en faveur de la double origine des nematodes nearctiques du genre Heligmosomoides Hall, 1916. Annales de Parasitologie (Paris) 47, 365–382.Google Scholar
Ehrenford, F.A. (1954) The life-cycle of Nematospiroides dubius Baylis (Nematoda: Heligmosomidae). Journal of Parasitology 40, 480–481.Google Scholar
Gregory, R.D., Keymer, A.E. & Clarke, J.R. (1990) Genetics, sex and exposure: the ecology of Heligmosomoides polygyrus (Nematoda) in the wood mouse. Journal of Animal Ecology 59, 363–378.CrossRefGoogle Scholar
Keymer, A.E. (1985) Experimental epidemiology: Nematospiroides dubius and the laboratory mouse. pp. 55–75in Rollinson, D. & Anderson, R.M. (Eds) Ecology and genetics of host-parasite interactions. London, Academic Press.Google Scholar
Lewis, J.W. (1968) Studies on the helminth parasites of the long-tailed field mouse, Apodemus sylvaticus sylvaticus from Wales. Journal of Zoology 154, 287–312.CrossRefGoogle Scholar
Lewis, J.W. (1987) Helminth parasites of British rodents and insectivores. Mammal Review 17, 81–93.Google Scholar
Lewis, J.W. & Twigg, G.I. (1972) A study of the internal parasites of small rodents from woodland areas in Surrey. Journal of Zoology 166, 61–77.CrossRefGoogle Scholar
Maniatis, T., Fritsch, E.F. & Sambrook, J. (1982) Molecular cloning: a laboratory manual. 468 pp. New York, Cold Spring Harbour Laboratory.Google Scholar
McCutchan, T.F., Simpson, A.J.G., Mullins, J.A., Sher, A., Nash, T.E., Lewis, F. & Richards, C. (1984) Differentiation of schistosomes by species, strain, and sex by using cloned DNA markers. Proceedings of the National Academy of Sciences, USA 81, 889–893.CrossRefGoogle ScholarPubMed
McManus, D.P. & Rishi, A.K. (1989) Genetic heterogeneity within Echinococcus granulosus: isolates from different hosts and geographical areas characterized with DNA probes. Parasitology 99, 19–29.Google Scholar
Monroy, F.G. & Enriquez, F.J. (1992) Heligmosomoides polygyrus: A model for chronic gastrointestinal helminthiasis. Parasitology Today 8, 49–54.Google Scholar
Munro, M.A., Reid, A.P. & Whitfield, P.J. (1990) Genomic divergence in the ecologically differentiated English freshwater and marine strains of Pomphorhynchus laevis (Acanthocephala: Palaeacanthocephala): a preliminary investigation. Parasitology 101, 451–454.Google Scholar
Quinnell, R.J., Behnke, J.M. & Keymer, A.E. (1991) Host specificity of and cross-immunity between two strains of Heligsmosomoides polygyrus. Parasitology 102, 419–427.CrossRefGoogle ScholarPubMed
Radice, A.D., Powers, T.O., Sandall, L.J. & Riggs, R.D. (1988) Comparison of mitochondrial DNA from the sibling species Heterodera glycines and H. schachtii. Journal of Nematology 18, 83–86.Google Scholar
Reid, A.P. & Hominick, W.M. (1992) Restriction fragment length polymorphisms within the ribosomal DNA repeat unit of British entomopathogenic nematodes (Rhabditida: Steinernematidae). Parasitology 105, 317–323.Google Scholar
Reid, A.P. & Hominick, W.M. (1993) Cloning of the rDNA repeat unit from a British entomopathogenic nematode (Steinernematidae) and its potential for species identification. Parasitology 107, 529–536.CrossRefGoogle Scholar
Rollinson, D., Walker, T.K. & Simpson, A.J.G. (1986) The application of recombinant DNA technology to problems of helminth identification. Parasitology 91 (Suppl.), S53–S71.CrossRefGoogle Scholar
Tenora, F. (1966) Some remarks on the species of the genus Heligmosomus Railliet et Henry, 1909 with an asymmetric bursa copulatrix. Folia Parasitologica 13, 205–211.Google Scholar
Wahl, G.M., Stern, M. & Stark, G.R. (1979) Efficient transfer of large DNA fragments from agarose gels to diazobenzy-loxymethyl-paper and rapid hybridization using dextran sulphate. Proceedings of the National Academy of Sciences, USA 76, 3683–3687.Google Scholar