Hostname: page-component-77c89778f8-swr86 Total loading time: 0 Render date: 2024-07-17T00:15:29.807Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunodiagnosis of human filariasis by counterimmunoelectrophoresis using Litomosoides carinii antigens

Published online by Cambridge University Press:  05 June 2009

A. Dasgupta ,
Shukal Bala ,
A. Banerjee  and
A. Chaudhury
A. Dasgupta
Affiliation:
Immunology Division, Department of Microbiology, All India Institute of Medical Sciences, New Delhi, 16, India
Shukal Bala
Affiliation:
Immunology Division, Department of Microbiology, All India Institute of Medical Sciences, New Delhi, 16, India
A. Banerjee
Affiliation:
Immunology Division, Department of Microbiology, All India Institute of Medical Sciences, New Delhi, 16, India
A. Chaudhury
Affiliation:
Immunology Division, Department of Microbiology, All India Institute of Medical Sciences, New Delhi, 16, India
Get access Rights & Permissions [Opens in a new window]

Abstract

The presence of precipitin antibody and soluble circulating antigen was demonstrated in sera of individuals with clinical filariasis by counterimmunoelectrophoresis. Antigens derived from Litomosoides carinii and antisera raised in rabbits to the adult worm antigens were used for the detection of antibody and antigen in human sera. Of 35 subjects, 2 had microfilariae in the peripheral blood, 9 soluble circulating antigen, 19 antibody in sera, and 6 had both antigen and antibody. It suggests that 5.7% of cases diagnosed as bancroftian filariasis by parasitological examination can be increased to 62.8% by serological means.

Type
Research Papers
Information
Journal of Helminthology , Volume 54 , Issue 2 , June 1980 , pp. 83 - 86
Copyright
Copyright © Cambridge University Press 1980

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ambroise-Thomas, P. (1974) Immunological diagnosis of human filariasis: present possibilities, difficulties and limitations. Acta Tropica, 31, 108–128.Google ScholarPubMed
Ambroise-Thomas, P. and Kien, Truong T. (1974) Application of the indirect fluorescent antibody test on sections of adult filariae to the serodiagnosis, epidemiology and post-therapeutic surveillance of human filariasis. Annals of Tropical Medicine and Parasitology, 68, 434–452.Google Scholar
Ambroise-Thomas, P. (1975) Etude critique des procedes diagnostiques, parasitologiques et immunologiques des filarioses humaines. Annales de la Societé Belge de Medecine Tropicale, 55, 497–504.Google Scholar
Beaver, P. C. (1970) Filariasis without microfilariaemia. American Journal of Tropical Medicine and Hygiene, 19, 181.CrossRefGoogle Scholar
Capron, A., Gentilini, M. and Vernes, A. (1968) Structure antigenique des helminthes – Aspects immunologiques des relations hote-parasite. Pathologie Biologique (Paris), 16, 121–138.Google Scholar
Dasgupta, A. and Bala, S. (1978a) Litomosoides carinii: soluble antigen in circulation and immunosuppression in vitro. Indian Journal of Medical Research, 67, 30–33.Google ScholarPubMed
Dasgupta, A. and Bala, S. (1978b) Enzyme linked immunosorbent assay in the serodiagnosis of filariasis. Progress in Filariasis (ed. A, Dasgupta); Ankur Publishing House, 1, 125–127.Google Scholar
Dasgupta, A. and Bala, S. (1978c) Immune complexes in sera of patients with bancroftian filariasis. Progress in Filariasis (ed A, Dasgupta); Ankur Publishing House, 1, 93–94.Google Scholar
Dasgupta, A. and Bala, S. (1978d) T-cell unresponsiveness in rat infected with Litomosoides carinii. Progress in filariasis (ed A, Dasgupta); Ankur Publishing House, 1, 87–89.Google Scholar
Dasgupta, A. and Bala, S. (1978e) T-cell unresponsiveness in bancroftian filariasis. Progress in Filariasis (ed A, Dasgupta); Ankur Publishing House, 1, 107–109.Google Scholar
Denham, D. A. (1975) The diagnosis of filariasis. Annales de la Societé Belge de Medecine Tropicale, 55, 517–524.Google ScholarPubMed
Desowitz, R. S. and Una, S. R. (1976) The detection of antibodies in human and animal filariasis by counterimmunoelectrophoresis with Dirofilaria immitis antigens. Journal of Helminthology, 50, 53–57.CrossRefGoogle ScholarPubMed
Diesfeld, H. J. and Kirsten, C. (1975) Localisation von Antigenitat adulter D viteae: Wurmer in der indirekten Immunofluoreszenz mit Filariase – Patientenserum. Tropenmedizin und Parasitologie, 26, 499–502.Google Scholar
Fujita, K., Tanaka, H., Sasa, M., Skickinoke, K., Asai, Y. and Kurokaxa, K. (1970) Cross reaction among filarial species in haemaggluitination test. Japanese Journal of experimental Medicine, 40, 67–77.Google ScholarPubMed
Niel, G., Gidel, R., Couture, J., Pinon, J. M. and Gentilini, M. (1972) Etude d’un extrait antigenique de la filaire Setaria labiato-papillosa en double diffusion et en immunoelectrophoreses applique au diagnostic immunologique des filarioses. Medecine et Malades infectieuses, 2, 193–202.CrossRefGoogle Scholar
Price, E. W. (1974) Endemic elephantiasis of the lower legs: natural history and clinical study. Transactions of the Royal Society of Tropical Medicine and Hygiene, 68, 44–52.CrossRefGoogle ScholarPubMed
Wheeling, G. H. and Hutchinson, W. F. (1971) Disc electrophoresis of soluble extracts of Dirofilaria immitis. Japanese Journal of experimental Medicine, 41, 171–176.Google ScholarPubMed
Zahner, H. (1974) L. carinii – Infection des Mastomys natalensis: serologische verlaufsuntersuchungen mit der Komplementbindungreaktion und indirekten Haemagglutination, Zeitschrift für Parasitenkunde, 43, 181–196.CrossRefGoogle Scholar