Hostname: page-component-5c6d5d7d68-wpx84 Total loading time: 0 Render date: 2024-08-22T04:28:47.476Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunological Studies on Experimental Infection of Guineapigs and Rabbits with Ascaris suum Goeze, 1782. III.—The Antibody Response in Rabbits Demonstrated by Means of the Complement Fixation and Conglutinating Complement Absorption Tests*

Published online by Cambridge University Press:  18 November 2009

L. F. Taffs
L. F. Taffs
Affiliation:
Department of Animal Pathology, School of Veterinary Medicine, Cambridge**
Get access Rights & Permissions [Opens in a new window]

Extract

Antibodies were demonstrated in the sera of rabbits experimentally infected by mouth with the eggs of A. suum.

An alcoholic extract of freeze-dried ovaries, oviducts and uteri of adult female Ascaris worms was used as an antigen in the complement fixation test, and a polysaccharide fraction and a saline extract of whole worms were used as antigens in the conglutinating complement absorption test.

In all rabbits the pattern and nature of the antibody response was similar using the three different antigens. When the saline extract antigen was used in the C.C.A.T., antibodies were detected earlier, and titres were recorded higher, than when other systems were used. The degree of response was proportional to the size of the infective dose. Circulating antibody first appeared between the third and eighth days of infection. The highest concentration of serum antibody was reached between the eighth and twenty-first days, and in those animals which received large doses the maximum response occurred about the third week. By repeated twice-weekly infection antibody was demonstrated in the serum for 114 days.

Type
Research Papers
Information
Journal of Helminthology , Volume 38 , Issue 3-4 , December 1964 , pp. 325 - 348
Copyright
Copyright © Cambridge University Press 1964

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Blackie, W. K., 1931—“Observations on the immunity response in experimental ascariasis in rabbits.” J. Helminth., 9, 91–96.CrossRefGoogle Scholar
Campbell, D. H., 1937—“The immunological specificity of a polysaccharide fraction from some common parasitic helminths.” J. Parasit., 23, 348–353.CrossRefGoogle Scholar
Canning, G. A., 1929—“Precipitin reactions with various tissues of Ascaris lumbricoides and related helminths.” Amer. J. Hyg., 9, 207–220.Google Scholar
Coventry, F. A., 1929—“Hypersensitiveness to helminth proteins. II. Cutaneous and precipitin tests with Ascaris extracts in infected and immunized animals.” J. prev. Med., Baltimore, 3, 43–62.Google Scholar
Fblton, L. D., 1940—“Immunization against pneumonia.” Amer. J. publ. Hlth., 30, 361–368CrossRefGoogle Scholar
Fblton, L. D., Kauffmann, G., Prescott, B. and Ottinger, B., 1955.—“Studies on the mechanism of the immunological paralysis induced in mice by pneumococcal polysaccharides.” J. Immunol., 74, 17–20.CrossRefGoogle Scholar
Jezioranska, A. and Dodrowolska, H., 1950.—“Odczyny immunologiczne przy askarodozie.” Wiad. Paraxyt., 2 (5), Suppl., 117–118. (Biol. Abs. 1957, Sect. C, 31, No. 7, Entry No. 21827).Google Scholar
Jezioranska, A. and Dodrowolska, H., 1957.—“Odczyny immunologiczne w glistnicy.” Med. Dósw. Mihrobiol., 9 (2), 107–177. (Helminth. Abs., 26. No. 119a).Google Scholar
Kabat, E. A. and Mayer, M. M., 1948.—“Experimental immunochemistry.” p. 107, Thomas, Illinois.Google Scholar
Oliver-Gonzalez, J., 1943.—“Antigenic analysis of the isolated tissues and body fluids of the roundworm, Ascaris lumbricoides var. suum.” J. infect. Dis., 72, 202–212.CrossRefGoogle Scholar
Oliver-Gonzalez, J., 1940.—“Functional antigens in helminths.” J. infect. Dis., 78. 232–237.CrossRefGoogle Scholar
Raffel, S., 1901.—“Immunity.” (Second edition), Appleton-Ccntury-Crofts, Inc., New York.Google Scholar
Richardson, G. M., 1941.—“Preservation of liquid complement serum.” Lancet, 2. 696–697.CrossRefGoogle Scholar
Soulsby, E. J. L., 1957.—“Antigenic analysis of Ascaris tissues by the double diffusion precipitin test.” Trans. R. Soc. trop. Med. Hyg., 51, 9–10.Google Scholar
Taffs, L. F., 1963.—“Immunological studies on experimental infection of pigs with Ascaris suum Goeze, 1782. II. The use of the conglutinating complement absorption test for the demonstration of Ascaris antibody.” J. Helminth., 37, 369–380.CrossRefGoogle ScholarPubMed
Taffs, L. F., 1964a.—“Immunological studies on experimental infection of guineapigs and rabbits with Ascaris suum Goeze, 1782. I. The minimum lethal dose (M.L.D.) and the minimum immunizing dose (M.I.D.) of Ascaris eggs in guineapigs.” J. Helminth., 38, 303–314.CrossRefGoogle Scholar
Taffs, L. F., 1964b.—“Immunological studies on experimental infection of guineapigs and rabbits with Ascaris suum Goeze, 1782. II. Antibody production and the further demonstration of acquired resistance in guineapigs.” J. Helminth., 38, 315–324.CrossRefGoogle ScholarPubMed