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Prevalence and molecular characterization of Spirometra erinaceieuropaei spargana in snakes in Hunan Province, China

Published online by Cambridge University Press:  27 February 2020

W. Liu ,
L. Tan ,
Y. Huang ,
W.C. Li ,
Y.S. Liu  and
L.C. Yang Open the ORCID record for L.C. Yang [Opens in a new window]
W. Liu
Affiliation:
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan410128, China Hunan Co-Innovation Center of Animal Production Safety, Changsha, China College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
L. Tan
Affiliation:
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan410128, China Hunan Co-Innovation Center of Animal Production Safety, Changsha, China
Y. Huang
Affiliation:
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
W.C. Li
Affiliation:
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan410128, China
Y.S. Liu
Affiliation:
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan410128, China Hunan Co-Innovation Center of Animal Production Safety, Changsha, China
L.C. Yang*
Affiliation:
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan410128, China Hunan Co-Innovation Center of Animal Production Safety, Changsha, China
*
Author for correspondence: L. Yang, E-mail: lcyang@hunau.edu.cn
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Abstract

Sparganosis is an important foodborne parasitic zoonosis; however, few reports on the prevalence of snake-infecting plerocercoids from Hunan province in China are available. Therefore, we investigated the prevalence of spargana infection in wild snakes from this region in 2018, and identified an astonishing prevalence rate of 91.7% (344/375). Spargana parasites were found in 99.1% of Zaocys dhumnades, 94.1% of Elaphe carinata and 86.7% of Elaphe taeniura. Parasites exhibited various distributions: 50% were located in muscular tissue, 32.1% in subcutaneous tissue and 17.9% in the coelomic cavity. To identify the specific status of spargana collected from wild snakes, partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequences were amplified, sequenced and analysed. Sequence variations for cox1 among all the examined plerocercoids ranged between 0.0 and 2.9%, with 21 variable sites identified (4.71%, 21/446). Phylogenetic analyses identified that all plerocercoids isolated from Hunan province were Spirometra erinaceieuropaei. This is the first report of S. erinaceieuropaei infection in snakes in Hunan province. The risks and harms of sparganosis should be publicized, and illegal wildlife trade should be controlled.

Keywords

Hunan provincepleroceroidSpirometra einaceieuropaeimitochondrial genephylogenetic analysis
Type
Short Communication
Information
Journal of Helminthology , Volume 94 , 2020 , e131
Copyright
Copyright © The Author(s) 2020. Published by Cambridge University Press

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