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A comparison of a modified quick-Kato technique and the Stoll dilution method for field examination for Opisthorchis viverrini eggs*

Published online by Cambridge University Press:  05 June 2009

V. Viyanant ,
W. Y. Brockelman ,
P. Lee ,
S. Ardsungnoen  and
E. S. Upatham
V. Viyanant
Affiliation:
Department of Biology, Faculty of Science, Mahidol University, Bangkok 4, Thailand
W. Y. Brockelman
Affiliation:
Department of Biology, Faculty of Science, Mahidol University, Bangkok 4, Thailand
P. Lee
Affiliation:
Department of Biology, Faculty of Science, Mahidol University, Bangkok 4, Thailand
S. Ardsungnoen
Affiliation:
Department of Biology, Faculty of Science, Mahidol University, Bangkok 4, Thailand
E. S. Upatham
Affiliation:
Department of Biology, Faculty of Science, Mahidol University, Bangkok 4, Thailand
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Abstract

A modified quick-Kato technique and the Stoll dilution method for the diagnosis of Opisthorchis viverrini infection were compared from the standpoints of both replicability and sensitivity. One Stoll dilution preparation and two modified quick-Kato smears were prepared from single stool specimens collected from 221 persons in a village in north-eastern Thailand. Stool examinations were made in duplicate for each technique by two examiners, and the averages of the raw egg counts were used to estimate the intensity of infection. Statistical analyses revealed that the modified quick-Kato technique was slightly more sensitive, and thus would detect more positives, but was lacking in replicability and hence less suitable for measurement of individual intensity of infection. We therefore recommend the Stoll dilution technique for field surveys and measurement of individual intensity of 0. viverrini infection.

Type
Research Papers
Information
Journal of Helminthology , Volume 57 , Issue 3 , September 1983 , pp. 191 - 195
Copyright
Copyright © Cambridge University Press 1983

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References

REFERENCES

Harinasuta, C. & Vajrasthira, S. (1960) Opisthorchiasis in Thailand. Annuls of Tropical Medicine and Parasitology, 54, 100–105.CrossRefGoogle ScholarPubMed
Katz, N., Chaves, A. & Pellegrino, J. (1972) A simple device for quantitative stool thick-smear technique in schistosomiasis mansoni. Revistu do Instituto de Medicina Tropical de Sao Paulo, 14, 397–400.Google ScholarPubMed
Martin, L.K. (1965) Randomness of particle distribution in human faeces and the resulting influence of helminth egg counting. American Journal of Tropical Medicine and Hygiene, 14, 747–759.CrossRefGoogle ScholarPubMed
Martin, L.K. & Beaver, P.C. (1968) Evaluation of Kato thicksmear technique for qualitative diagnosis of helminth infections. American Journal of Tropical Medicine and Hygiene, 17, 382–391.CrossRefGoogle Scholar
Papasarathorn, T., Chularerk, P., Chearanai, S., Chularerk, U., Kiarttivuti, B., Viboolyavatna, J., Kiarttivuti, A. & Vongtangswad, S. (1967) Study on ecology and prevalence of intestinal parasites with special reference to the intensity of hookworm infection and opisthorchiasis in health development area, Bantard, Udorn Province (In Thai with English abstract). Journal of the Medical Association of Thailand, 50, 423–445.Google Scholar
Peters, P.A., El Alamy, M., Warren, K.S. & Mahmoud, A.A.F. (1980) Quick Kato smear for field identification of Schistosoma mansoni eggs. American Journal of Tropical Medicine and Hygiene, 29, 217–219.CrossRefGoogle Scholar
Ritchie, L.S. (1948) An ether sedimentation technique for routine stool examination. Bulletin of the United States Army Medical Department, 8, 326–331.Google Scholar
Sadun, E.H. (1955) Studies on Opisthorchis viverrini in Thailand. American Journal of Hygiene, 62, 81–115.Google ScholarPubMed
Stoll, N.R. (1923) An effective method of counting hookworm eggs in feces. American Journal of Hygiene, 3, 59–70.Google Scholar
Stoll, N.R. & Hausheer, W.C. (1926) Accuracy in the dilution eggcounting method. American Journal of Hygiene, 6, 80–133.Google Scholar
Upatham, E.S, Viyanant, V., Kurathong, S., Brockelman, W.Y., Menaruchi, A., Saowakontha, S., Intarakhao, C., Vajrasthira, S. & Warren, K.S. (1982) Morbidity in relation to intensity of infection in Opisthorchis viverrini: Study of a community in Khon Kaen, Thailand. American Journal of Tropical Medicine and Hygiene, 31, 1156–1163.CrossRefGoogle ScholarPubMed
Vajrasthira, S. & Harinasuta, C. (1957) Study on helminthic infections in Thailand. I. Incidence, distribution and epidemiology of seven common intestinal helminths (in Thai with English abstract). Journal of the Medical Association of Thailand, 40, 309–340.Google Scholar
Viyanant, V. (1981) Opisthorchiasis in Thailand; a review. Journal of Parasitology and Tropical Medicine Association of Thailand, 4, 87–106.Google Scholar
Wykoff, D.E., Chittayasothorn, K. & Winn, M.M. (1966) Clinical manifestations of Opisthorchis viverrini infection in Thailand. American Journal of Tropical Medicine and Hygiene, 15, 914–918.CrossRefGoogle Scholar
Wykoff, D.E, Harinasuta, C., Juttijudata, P. & Winn, M.M. (1965) Opisthorchis viverrini in Thailand. The life cycle and comparison with O.felineus. Journal of Parasitology, 51, 207–214.CrossRefGoogle ScholarPubMed