Hostname: page-component-7479d7b7d-t6hkb Total loading time: 0 Render date: 2024-07-08T09:00:10.457Z Has data issue: false hasContentIssue false
  • English
  • Français

Description of Metagonimus pusillus sp. nov. (Trematoda: Heterophyidae): phylogenetic relationships within the genus

Published online by Cambridge University Press:  22 December 2017

Y.V. Tatonova ,
P.G. Shumenko  and
V.V. Besprozvannykh
Y.V. Tatonova*
Affiliation:
Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, 100-letiya Street, 159, Vladivostok, 690022, Russia
P.G. Shumenko
Affiliation:
Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, 100-letiya Street, 159, Vladivostok, 690022, Russia
V.V. Besprozvannykh
Affiliation:
Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, 100-letiya Street, 159, Vladivostok, 690022, Russia
*
Author for correspondence: Y.V. Tatonova, E-mail: ytatonova@gmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

As a result of experimental studies conducted in the Russian southern Far East, adult worms from the genus Metagonimus were obtained. A comparative analysis of the morphometry of these worms with other Metagonimus representatives showed that they are most similar to M. katsuradai Izumi, 1935 and M. otsurui Shimazu & Urabe, 2002 found in Japan, due to the ratio of suckers and the positions of the testicle, uterus and vitellaria. However, Russian worms differ from species in Japan by other metric characters: they differ from M. otsurui by the maximum size of most organs and from M. katsuradai by body width, pharynx length, and maximum size of testes and ovary. At the same time, they are identical to a trematode from the Russian southern Far East, which was previously identified as M. katsuradai. The validity of this species was also confirmed by genetic data. According to the 28S gene and the internal transcribed spacer 2 (ITS2) region of rDNA, as well as the cytochrome c oxidase I (cox1) gene of mtDNA, the Metagonimus specimens found in Russia differ from published genetic data for other members of this genus. However, both morphological similarity and molecular data showed that M. pusillus sp. nov., M. katsuradai and M. otsurui are most likely cryptic species. Furthermore, additional data based on a mitochondrial marker were provided for M. suifunensis Shumenko, Tatonova & Besprozvannykh, 2017 from Russia.

Type
Research Paper
Information
Journal of Helminthology , Volume 92 , Issue 6 , November 2018 , pp. 703 - 712
Copyright
Copyright © Cambridge University Press 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Besprozvannykh, VV (2000) Fauna, biology, and ecology of parthenites and cercariae of trematodes in snails of genus Juga (Pachychilidae) from rivers in Primorsky Region. Vladivostok, Dalnauka.Google Scholar
Besprozvannykh, VV and Ermolenko, AV (2005) Human helminthosis from natural foci in Primorsky Region. Vladivostok, Dalnauka.Google Scholar
Besprozvannykh, VV, Ermolenko, AV and Dvorjadkin, VA (1987) On the discovery of Metagonimus katsuradai Isumi, 1935, (Trematoda, Heterophyidae) in southern Primorye. pp. 47–52 in Mamaev YL (Ed.) Helminthes and diseases caused by them. Vladivostok, Academy of Science USSR.Google Scholar
Bowles, J and McManus, DP (1994) Genetic characterization of the Asian Taenia, a newly described taeniid cestode of humans. American Journal of Tropical Medicine and Hygiene 50, 33–44.Google Scholar
Chai, JY, Murrell, KD and Lymbery, AJ (2005) Fish-borne parasitic zoonoses: status and issues. International Journal for Parasitology 35, 1233–1254.Google Scholar
Darriba, D, Taboada, GL, Doallo, R and Posada, D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9, 772. doi: 10.1038/nmeth.2109Google Scholar
Ermolenko, AV and Besprozvannykh, VV (1987) Fauna of trematode metacercariae from fishes in Razdolnaya river. pp. 33–47 in Mamaev YL (Ed.) Helminths and diseases caused by them. Vladivostok, Academy of Science USSR.Google Scholar
Guindon, S and Gascuel, O (2003) A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology 52, 696–704.Google Scholar
Ito, J (1964a) A monograph of cercariae in Japan and adjacent territories. pp. 395–550 in Morishita K, Komiya Y and Matsubayashi H (Eds) Progress of medical parasitology in Japan. Vol. 1. Tokyo, Meguro Parasitological Museum.Google Scholar
Ito, J (1964b) Metagonimus and other human heterophyid trematodes. pp. 314–393 in Morishita K, Komiya Y and Matsubayashi H (Eds) Progress of medical parasitology in Japan. Vol. 1. Tokyo, Meguro Parasitological Museum.Google Scholar
Kino, H, Suzuki, T, Oishi, H, Suzuki, S, Yamagiwa, S and Ishiguro, M (2006) Geographical distribution of Metagonimus yokogawai and M. miyatai in Shizuoka Prefecture, Japan, and their site preferences in the sweetfish, Plecoglassus altivelis, and hamsters. Parasitology International 55, 201–206.Google Scholar
Komiya, Y (1965) Metacercariae in Japan and adjacent territories. pp. 1–328 in Morishita K, Komiya Y and Matsubayashi H (Eds) Progress of medical parasitology in Japan. Vol. 2. Tokyo, Meguro Parasitological Museum.Google Scholar
Morgan, JA and Blair, D (1995) Nuclear rDNA ITS sequence variation in the Trematode genus Echinostoma an aid to establishing relationships within the 37 collar-spine group. Parasitology 111, 609–615.Google Scholar
Morozov, FN (1952) Superfamily Heterophyoidea Faust, 1929. pp. 153–615 in Skrjabin, KI (Ed.) Trematodes of animals and man. Moscow, Academy of Science of USSR.Google Scholar
Оshmarin, PG (1963) Parasitic worms of mammals and birds in Primorsky Region. Moscow, Academy of Science of USSR.Google Scholar
Pornruseetairatn, S, Kino, H, Shimazu, T, Nawa, Y, Scholz, T, Ruangsittichai, J, Saralamba, NT and Thaenkham, U (2016) A molecular phylogeny of Asian species of the genus Metagonimus (Digenea) – small intestinal flukes – based on representative Japanese populations. Parasitology Research 115, 1123–1130.Google Scholar
Ronquist, F and Huelsenbeck, JP (2003) MrBayes 3, Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.Google Scholar
Saito, S, Chai, JY, Kim, KH, Lee, SH and Rim, HJ (1997) Metagonimus miyatai sp. nov. (Digenea, Heterophyidae) a new intestinal trematode transmitted by freshwater fishes in Japan and Korea. Korean Journal of Parasitology 35, 223–232.Google Scholar
Shatrov, AS (1974) Biology of Metagonimus yokogawai Katsurada, 1912, in upper Amur region. Parasitology 10, 196–199.Google Scholar
Shimazu, T. (1999) Metagonimus hakubaensis sp. n. (Digenea, Heterophyidae) from Nagano, Japan: morphology and life cycle. Bulletin of the National Museum of Nature and Science, Series (Zoology), Tokyo 25, 87–99.Google Scholar
Shimazu, T (2003) Morphology of metacercariae and adults of Metagonimus katsuradai Izumi (Digenea, Heterophyidae) from Shiga, Japan. Bulletin of the National Museum of Nature and Science, Series (Zoology), Tokyo 25, 47–51.Google Scholar
Shimazu, T and Kino, H (2015) Metagonimus yokogawai (Trematoda, Heterophyidae). From discovery to designation of a neotype. Korean Journal of Parasitology 53, 627–639.Google Scholar
Shimazu, T and Urabe, M (2002) Morphology and life cycle of Metagonimus otsurui (Digenea, Heterophyidae) from Nara, Japan. Bulletin of the National Museum of Nature and Science, Series (Zoology), Tokyo 25, 21–28.Google Scholar
Shumenko, PG, Tatonova, YV and Besprozvannykh, VV (2017) Metagonimus suifunensis sp. n. (Trematoda, Heterophyidae) from the Russian Southern Far East: morphology, life cycle, and molecular data. Parasitology International 66, 982–991.Google Scholar
Skrjabin, KI, Podjapolskaya, VP and Schultz, RS (1930) Metagonimiasis of humans and animals in Far Eastern region. Tropical Medicine and Veterinary 6–7, 16–25.Google Scholar
Tamura, K, Peterson, D, Peterson, N, Stecher, G, Nei, M and Kumar, S (2011) MEGA5, molecular evolutionary genetic analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Molecular Biology and Evolution 28, 2731–2739.Google Scholar
Thaenkham, U, Nawa, Y, Blair, D, and Pakdee, W (2011) Confirmation of the paraphyletic relationship between families Opisthorchiidae and Heterophyidae using small and large subunit ribosomal DNA sequences. Parasitology International 60, 521–523.Google Scholar
Tkach, VV, Littlewood, DTJ, Olson, PD, Kinsella, JM and Swiderski, Z (2003) Molecular phylogenetic analysis of the Microphalloidea Ward, 1901, (Trematoda, Digenea). Systematic Parasitology 56, 1–15.Google Scholar
Truett, GE, Heeger, P, Mynatt, RL, Truett, AA, Walker, JA and Warman, ML (2000) Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and Tris (HotSHOT). Biotechniques 29, 52–54.Google Scholar
Vilas, R, Criscione, CD and Blouin, MS (2005) A comparison between mitochondrial DNA and the ribosomal internal transcribed regions in prospecting for cryptic species of platyhelminth parasites. Parasitology 131, 1–8.Google Scholar
Yu, YR and Chai, JY (2012) Metagonimus. pp. 389–398 in Liu, D. (Ed.) Molecular detection of human parasitic pathogens. Boca Raton, CRC Press.Google Scholar
Supplementary material: File

Tatonova et al supplementary material

Figure S1 and Tables S1-S3

Download Tatonova et al supplementary material(File)
File 4.8 MB