Hostname: page-component-848d4c4894-sjtt6 Total loading time: 0 Render date: 2024-06-29T16:54:50.812Z Has data issue: false hasContentIssue false
  • English
  • Français

Enzyme-linked immunosorbent assay with worm vomit and cercarial secretions of Schistosoma mansoni to detect infections in an endemic focus of Burkina Faso

Published online by Cambridge University Press:  12 April 2024

M. Bahgat ,
H. Sorgho ,
J.B. Ouédraogo ,
J.N. Poda ,
L. Sawadogo  and
A. Ruppel
M. Bahgat
Affiliation:
Department of Tropical Hygiene and Public Health, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
H. Sorgho
Affiliation:
Department of Tropical Hygiene and Public Health, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Burkina Faso
J.B. Ouédraogo
Affiliation:
Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Burkina Faso
J.N. Poda
Affiliation:
Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Burkina Faso
L. Sawadogo
Affiliation:
UFR-SVT, Université de Ouagadougou, Burkina Faso.
A. Ruppel*
Affiliation:
Department of Tropical Hygiene and Public Health, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
*
* Author for correspondence Fax: +49 6221 565948, E-mail: Andreas.Ruppel@urz.uni-heidelberg.de.
Get access Rights & Permissions [Opens in a new window]

Abstract

Cercariae and adult Schistosoma mansoni were used to prepare, respectively, cercarial secretions (CS) and worm vomit (WoV). These were used as antigens in an enzyme-linked immunosorbent assay (ELISA) to test the IgG-reactivity of sera obtained in an S. mansoni-endemic area of Burkina Faso. Among the egg-excreting individuals (n=240), 94.6% reacted positively with WoV, but only 62.9% with CS, thus suggesting a high diagnostic sensitivity of WoV, but not of CS. Among those individuals without detectable eggs in two Kato-Katz thick smears from different stool specimens (n=215), the respective percentages of positive IgG reactivity were 78.1% and 63.3%. These positive reactions in the absence of detectable eggs are interpreted in terms of limited sensitivity of parasitological stool examinations. Optical density values in ELISA with CS, but not with WoV, correlated negatively with age, which may reflect decreasing exposure to cercariae in older individuals.

Type
Research Article
Information
Journal of Helminthology , Volume 80 , Issue 1 , March 2006 , pp. 19 - 23
Copyright
Copyright © Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

Department of Medicinal Chemistry and Central Laboratory, Division of Pharmaceutical Industries and Drug Research, National Research Center, Dokki, Cairo, Egypt.

References

Bahgat, M., Francklow, K., Doenhoff, M.J., Li, Y.L., Ramzy, R.M.R., Kirsten, C. & Ruppel, A. (2001) Infection induces antibodies against the cercarial secretions, but not against the cercarial elastases of Schistosoma mansoni, Schistosoma haematobium, Schistosoma japonicum and Trichobilharzia ocellata . Parasite Immunology 23, 557–565.CrossRefGoogle Scholar
Bergquist, R., Al-Sherbiny, M., Barakat, R. & Olds, R. (2002) Blueprint for schistosomiasis vaccine development. Acta Tropica 82, 183–192.CrossRefGoogle ScholarPubMed
Bergquist, N.R., Leonardo, L.R. & Mitchell, G.F. (2005) Vaccine-linked chemotherapy: can schistosomiasis control benefit from an integrated approach? Trends in Parasitology 21, 112–117.CrossRefGoogle ScholarPubMed
Cutts, L. & Wilson, R.A. (1997) The protein antigens secreted in vitro by adult male Schistosoma mansoni . Parasitology 114, 245–255.CrossRefGoogle Scholar
Caffrey, C.R. & Ruppel, A. (1997) Cathepsin B-like activity predominates over cathepsin L-like activity in adult Schistosoma mansoni and S. japonicum . Parasitology Research 83, 632–635.CrossRefGoogle ScholarPubMed
Caffrey, C.R., McKerrow, J.H., Salter, J.P. & Sajid, M. (2004) Blood ‘n’ guts: an update on schistosome digestive peptidases. Trends in Parasitology 20, 241–248.CrossRefGoogle ScholarPubMed
De Vlas, S.J. & Gryseels, B. (1992) Underestimation of Schistosoma mansoni prevalences. Parasitology Today 8, 274–277.CrossRefGoogle ScholarPubMed
Doenhoff, M.J., Wheeler, J.G., Tricker, K., Hamilton, J.V., Sturrock, R.F., Butterworth, A.E., Ouma, J.H., Mbugua, G.G., Kariuki, C. & Koech, D. (2003) The detection of antibody against Schistosoma mansoni soluble egg antigen (SEA) and CEF6 in ELISA, before and after chemotherapy. Annals of Tropical Medicine and Parasitology 97, 697–709.CrossRefGoogle ScholarPubMed
Doenhoff, M.J., Chiodini, P.L. & Hamilton, J.V. (2004) Specific and sensitive diagnosis of schistosome infection: can it be done with antibodies? Trends in Parasitology 20, 35–39.CrossRefGoogle ScholarPubMed
Engels, D., Sinzinkayo, E. & Gryseels, B. (1996) Day-to-day egg count fluctuation in Schistosoma mansoni infection and its operational implications. American Journal of Tropical Medicine and Hygiene 54, 319–324.CrossRefGoogle ScholarPubMed
Engels, D., Chitsulo, L., Montresor, A. & Savioli, L. (2002) The global epidemiological situation of schistosomiasis and new approaches to control and research. Acta Tropica 82, 139–146.CrossRefGoogle ScholarPubMed
Fenwick, A., Savioli, L., Engels, D., Bergquist, N.R. & Todd, M.H. (2003) Drugs for the control of parasitic diseases: current status and development in schistosomiasis. Trends in Parasitology 19, 509–515.CrossRefGoogle ScholarPubMed
Hagan, P., Appleton, C.C., Coles, G.C., Kusel, J.R. & Tchuem-Tchuente, L.A. (2004) Schistosomiasis control: keep taking the tablets. Trends in Parasitology 20, 92–97.CrossRefGoogle ScholarPubMed
Hamilton, J.V., Klinkert, M. & Doenhoff, M.J. (1998) Diagnosis of schistosomiasis: antibody detection, with notes on parasitological and antigen detection methods. Parasitology 177, S41–S57.Google Scholar
Harrop, R., Jennings, N., Mountford, A.P., Coulson, P.S. & Wilson, R.A. (2000) Characterization, cloning and immunogenicity of antigens released by transforming cercariae of Schistosoma mansoni . Parasitology 121, 385–394.CrossRefGoogle ScholarPubMed
Idris, M.A., Shaban, M., Richter, J., Moné, H., Mouahid, G. & Ruppel, A. (2003) Emergence of infections with Schistosoma mansoni in the Dhofar Governorate, Oman. Acta Tropica 88, 137–144.CrossRefGoogle ScholarPubMed
Katz, N., Chave, A. & Pellegrino, J. (1972) A simple device for quantitative stool thick smear technique in schistosomiasis mansoni. Revista do Instituto de Medicina Tropical de Sao Paulo 14, 397–400.Google ScholarPubMed
Klinkert, M.Q., Felleisen, R., Link, G., Ruppel, A. & Beck, E. (1989) Primary structures of Sm31/32 diagnostic proteins and their identification as proteases. Molecular and Biochemical Parasitology 33, 113–122.CrossRefGoogle ScholarPubMed
Kongs, A., Marks, G., Verlé, P. & van der Stuyft, P. (2001) The unreliability of the Kato-Katz technique limits its usefulness for evaluating S. mansoni infections. Tropical Medicine and International Health 6, 163–169.CrossRefGoogle ScholarPubMed
Li, Y.L., Herter, U. & Ruppel, A. (2004) Acute, chronic and late-stage infections with Schistosoma japonicum: reactivity of patient sera in indirect immunofluorescence tests. Annals of Tropical Medicine and Parasitology 98, 49–57.CrossRefGoogle ScholarPubMed
Mott, K.S. & Dixon, H. (1982) Collaborative study on antigens for immunodiagnosis of schistosomiasis. Bulletin of the World Health Organization 60, 729–753.Google Scholar
Nash, T.E. & Deelder, A.M. (1985) Comparison of four schistosome excretory-secretory antigens: phenol-sulfuric test active peak, cathodic circulating antigen, gut-associated proteoglycan, and circulating anodic antigen. American Journal of Tropical Medicine and Hygiene 34, 236–241.CrossRefGoogle ScholarPubMed
Ramzy, R.M.R., Faris, R., Bahgat, M., Helmy, H., Franklin, C. & McKerrow, J.H. (1997) Evaluation of a stage-specific proteolytic enzyme of Schistosoma mansoni as a marker of exposure. American Journal of Tropical Medicine and Hygiene 56, 668–673.CrossRefGoogle ScholarPubMed
Ruppel, A., Diesfeld, H.J. & Rother, U. (1985) Immunoblot analysis of sera of Schistosoma mansoni antigens with sera of schistosomiasis patients: diagnostic potential of an adult schistosome protein. Clinical and Experimental Immunology 62, 499–506.Google Scholar
Ruppel, A., Rother, U., Vongerichten, H., Lucius, R. & Diesfeld, H.J. (1985) Schistosoma mansoni: immunoblot analysis of adult worm proteins. Experimental Parasitology 60, 195–206.CrossRefGoogle ScholarPubMed
Ruppel, A., Idris, M.A., Sulaiman, S.M. & Hilali, A.M.H. (1990) Schistosoma mansoni diagnostic antigens (Sm31/32): a seroepidemiological study in the Sudan. Tropical Medicine and Parasitology 41, 127–130.Google Scholar
Sorgho, H., Bahgat, M., Poda, J.N., Song, W.J., Kirsten, C., Doenhoff, M.J., Zongo, I., Ouédraogo, J.B. & Ruppel, A. (2005) Serodiagnosis of Schistosoma mansoni infections in an endemic area of Burkina Faso: performance of several immunological tests with different parasite antigens. Acta Tropica 93, 169–180.CrossRefGoogle Scholar
Tarp, B., Black, F.T. & Petersen, E. (2000) The immunofluorescence antibody test (IFAT) for the diagnosis of schistosomiasis used in a non-endemic area. Tropical Medicine and International Health 5, 185–191.CrossRefGoogle Scholar
Utzinger, J. & Keiser, J. (2004) Schistosomiasis and soil-transmitted helminthiasis: common drugs for treatment and control. Expert Opinion on Pharmacotherapy 5, 263–285.CrossRefGoogle ScholarPubMed
Utzinger, J., Booth, M., N'Goran, E.K., Muller, I., Tanner, M. & Lengeler, C. (2001) Relative contribution of day-to-day and intra-specimen variation in faecal egg counts of Schistosoma mansoni before and after treatment with praziquantel. Parasitology 122, 537–544.CrossRefGoogle ScholarPubMed
Van Lieshout, L., Polderman, A.M. & Deelder, A.M. (2000) Immunodiagnosis of schistosomiaisis by determination of the circulating CAA and CCA, in particular in individuals with recent or light infections. Acta Tropica 77, 69–80.CrossRefGoogle ScholarPubMed