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Identification of human hookworm in failed-treatment cases using Chinese hamsters (Cricetulus griseus) and scanning electron microscopy

Published online by Cambridge University Press:  05 June 2009

A. C. Evans ,
T. J. M. Daly  and
M. B. Markus
A. C. Evans
Affiliation:
Research Institute for Diseases in a Tropical Environment of the South African Medical Research Council, PO Box 634, Nelspruit 1200, South Africa
T. J. M. Daly
Affiliation:
Department of General Anatomy, University of the Witwatersrand, Johannesburg 2050, South Africa
M. B. Markus
Affiliation:
Department of Zoology, University of the Witwatersrand, Johannesburg 2050, South Africa
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Abstract

An attempt was made to identify the human hookworm involved in failed-treatment cases using abnormal hosts and scanning electron microscopy. Thirty-seven, 2 to 6 month old Chinese hamsters (Cricetulus griseus) from a closed, outbred, conventional colony, were each given between 20 and 120 filariform larvae per os. The larvae were cultured from faeces from mebendazole (Vermox®) 500 mg single-dose, failed-treatment cases living in the lowveld farming area of the Transvaal Province, South Africa. About 60 to 78 days after inoculation, the animals were killed and adult worms were removed from their small intestines. Eleven (30%) of the 37 hamsters harboured a total of 31 adult worms (19 males and 12 females), while 26 hamsters were refractory to infection. The greatest number of worms recovered from a single animal was six. A total of 27 worms (17 males and 10 females) were subjected to examination by scanning electron microscopy. Micrographs showed male and female worms to be morphologically all of the Necator americanus species, as identified by a pair of ventral and dorsal cutting plates, a dorsal tooth and the fused terminus of spicules in the male bursa. The transverse cuticular striations were distinct and smooth. Several points of interest arose from the results of this study and are discussed.

Keywords

mebendazoleNecator americanustreatment failuresCricetulus griseusscanning electron microscopyabnormal hostlarvaeMesocricetus auratushookworm
Type
Research Papers
Information
Journal of Helminthology , Volume 65 , Issue 1 , March 1991 , pp. 67 - 72
Copyright
Copyright © Cambridge University Press 1991

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