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The identity of Anisakis type II larvae with Anisakis physeteris confirmed by restriction fragment length polymorphism analysis of genomic DNA

Published online by Cambridge University Press:  05 June 2009

T. Matsuura
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3–1–1, Maysumoto City, Nagano Prefecture 390, Japan
S. Sun
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3–1–1, Maysumoto City, Nagano Prefecture 390, Japan
K. Sugane
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3–1–1, Maysumoto City, Nagano Prefecture 390, Japan

Abstract

The identity of Anisakis type II larvae with adult A. physeteris was confirmed by comparison of restriction fragment length polymorphisms (RFLPs) of 25S ribosomal DNA (rDNA). Patterns of RFLPs in larvae were almost identical with those in adult worms. Directly labelled 25S rDNA might serve as an appropriate probe with highly specific activity for examining RFLPs of larvae and adult worms.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1992

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