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Immunodiagnosis of human dirofilariasis by enzyme-linked immunosorbent assay using recombinant DNA-derived fusion protein

Published online by Cambridge University Press:  05 June 2009

S. Sun  and
K. Sugane
S. Sun
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3-1-1, Matsumoto City, Nagano Prefecture, Japan
K. Sugane*
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3-1-1, Matsumoto City, Nagano Prefecture, Japan
*
*Author to whom correspondence should be addressed.
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Abstract

An enzyme-linked immunosorbent assay (ELISA) using antigenic β-galactosidase-Dirofilaria immitis recombinant fusion protein (FP) obtained by the recombinant DNA technique provided a useful diagnostic tool for human dirofilariasis. D. immitis-infected human sera reacted strongly with FP that was immobilized with anti-β-galactosidase monoclonal antibody on microplates. However, the FP did not react with sera from patients with other filariasis. In detection of anti-D. immitis IgG antibody. ELISA using FP was highly sensitive and specific compared to that using crude somatic antigen.

Keywords

Dirofilaria immitisdiagnosisELISAfusion proteinNematoda
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 3 , September 1992 , pp. 220 - 226
Copyright
Copyright © Cambridge University Press 1992

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