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Morphology and molecular analysis of life-cycle stages of Proctoeces maculatus (Looss, 1901) (Digenea: Fellodistomidae) in the Bizerte Lagoon, Tunisia

Published online by Cambridge University Press:  23 December 2015

R. Antar  and
L. Gargouri
R. Antar*
Affiliation:
Unité de Recherche: Bio-Ecologie Animale et Systématique Evolutive, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092, Tunis, Tunisie
L. Gargouri
Affiliation:
Unité de Recherche: Bio-Ecologie Animale et Systématique Evolutive, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092, Tunis, Tunisie
*
*E-mail: rym.antar@hotmail.fr
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Abstract

The life cycle of Proctoeces maculatus (Looss, 1901) (Digenea, Fellodistomidae) was studied in Bizerte Lagoon (Tunisia). Three sequential hosts appear to be involved: the Mediterranean mussel Mytilus galloprovincialis Lamarck, 1819 (Mytilidae) as the first intermediate host; the polychaete Sabella pavonina Savigny, 1822 (Sabellidae), as the second intermediate host; and fishes (Lithognathus mormyrus (Linnaeus, 1758) (Sparidae), Trachinotus ovatus (Linnaeus, 1758) (Carangidae) and Sparus aurata Linnaeus, 1758 (Sparidae) as the definitive hosts. It should be noted that S. pavonina was recorded as second intermediate host for P. maculatus for the first time. Molecular confirmation of the morphological identification of the life-cycle stages of this digenean was obtained using partial 28S rDNA sequences. Comparative sequences revealed that the sporocysts and the metacercariae are conspecific but they diverged by 0.3% from the adults. The present results raised the possibility of the existence of cryptic species within the different developmental stages. However, all the present isolates differed from material from Archosargus probatocephalus in the Gulf of Mexico identified as P. maculatus.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 6 , November 2016 , pp. 726 - 736
Copyright
Copyright © Cambridge University Press 2015 

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