Hostname: page-component-5c6d5d7d68-thh2z Total loading time: 0 Render date: 2024-08-14T22:27:53.500Z Has data issue: false hasContentIssue false
  • English
  • Français

Neutral lipids in cercariae, encysted metacercariae, and rediae of Echinostoma caproni

Published online by Cambridge University Press:  12 April 2024

C.J. Marsit ,
B. Fried  and
J. Sherma
C.J. Marsit
Affiliation:
Department of Chemistry, Lafayette College, Easton, Pennsylvania, 18042, USA
B. Fried*
Affiliation:
Department of Biology, Lafayette College, Easton, Pennsylvania 18042, USA
J. Sherma
Affiliation:
Department of Chemistry, Lafayette College, Easton, Pennsylvania, 18042, USA
*
*Author for correspondence Fax: (610)330 5705 E-mail: friedb@lafayette.edu
Get access Rights & Permissions [Opens in a new window]

Abstract

High performance thin-layer chromatography (HPTLC) was used to analyse the neutral lipids in the rediae, cercariae, and encysted metacercariae of Echinostoma caproni from Biomphalaria glabrata snails. Visual observations of the chromatograms showed that the most abundant lipid fraction in all stages was free sterol. Quantification of the free sterol revealed mean weights of 2.7 ± 0.64 ng per redia, 0.53 ± 0.023 ng per cercaria, and 0.081 ± 0.0098 ng per encysted metacercaria. Oil Red O staining of the larval stages confirmed the presence of lipids within the rediae and cercariae but did not show lipids in the encysted metacercariae. The dimunition in neutral lipids from the cercarial to the encysted metacercarial stage does not support a previous observation that fat increases in successive phases of the digenean life cycle.

Type
Research Article
Information
Journal of Helminthology , Volume 74 , Issue 4 , December 2000 , pp. 365 - 367
Copyright
Copyright © Cambridge University Press 2000

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Beers, K., Fried, B., Fujino, T. & Sherma, J. (1995) Effects of diet on the lipid composition of the digestive gland-gonad complex of Biomphalaria glabrata (Gastropoda) infected with larval Echinostoma caproni (Trematoda). Comparative Biochemistry and Physiology 110, 729–737.CrossRefGoogle ScholarPubMed
Fried, B. & Huffman, J.E. (1996) The biology of the intestinal trematode Echinostoma caproni . Advances in Parasitology 38, 311–368.CrossRefGoogle ScholarPubMed
Fried, B., Eyster, L.S. & Pechenik, J.A. (1998) Histochemical glycogen and neutral lipid in Echinostoma trivolvis cercariae and effects of exogenous glucose on cercarial longevity. Journal of Helminthology 72, 83–85.CrossRefGoogle ScholarPubMed
Ginetsinskaya, T.A. (1988) Trematodes, their life cycles, biology, and evolution . New Delhi, Smerind Publishing Company, Pvt.Google Scholar
Muller, E.E, Fried, B. & Sherma, J. (1999) HPTLC determination of neutral lipids in the cercariae of Echinostoma trivolvis and Echinoparyphium sp. (Trematoda). Journal of Planar Chromatography-Modern TLC 12, 310–312.Google Scholar