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Quantitative real-time PCR (qPCR) for the detection and quantification of dactylogyrid parasites infecting Lutjanus guttatus

Published online by Cambridge University Press:  07 March 2017

L.C. Soler-Jiménez ,
A. García-Gasca  and
E.J. Fajer-Ávila
L.C. Soler-Jiménez
Affiliation:
Posgrado en Ciencias del Mar y Limnología, Unidad Mazatlán, Universidad Nacional Autónoma de México, Avenida Joel Montes Camarena s/n, Mazatlán, Sinaloa, C.P. 82040, México Laboratorio de Parasitología, Centro de Investigación en Alimentación y Desarrollo, A.C. Unidad Mazatlán en Acuicultura y Manejo Ambiental, Avenida Sábalo Cerritos s/n, Mazatlán, Sinaloa, C.P. 82010, México Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Unidad Mérida. Merida, Yucatan, C.P. 97310, México
A. García-Gasca
Affiliation:
Laboratorio de Parasitología, Centro de Investigación en Alimentación y Desarrollo, A.C. Unidad Mazatlán en Acuicultura y Manejo Ambiental, Avenida Sábalo Cerritos s/n, Mazatlán, Sinaloa, C.P. 82010, México
E.J. Fajer-Ávila*
Affiliation:
Laboratorio de Parasitología, Centro de Investigación en Alimentación y Desarrollo, A.C. Unidad Mazatlán en Acuicultura y Manejo Ambiental, Avenida Sábalo Cerritos s/n, Mazatlán, Sinaloa, C.P. 82010, México
*
*E-mail: efajer@ciad.mx
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Abstract

Severe infections of the spotted rose snapper Lutjanus guttatus resulting from dactylogyrid monogeneans present a risk to aquaculture. Currently, the diagnosis of this infection requires the morphological identification and manual quantification of parasites. Based on the characterization of the 28S rRNA gene of dactylogyrid species present in L. guttatus, specific primers were designed for real-time polymerase chain reaction (qPCR) using EvaGreen® chemistry. The standard curve method estimated the number of dactylogyrids accurately. A total of 85 gill samples from cage-cultured fish infected with dactylogyrids were analysed. The estimated number of dactylogyrids using this molecular method was very similar to the manual count that was performed initially. The standardized qPCR approach will be helpful as a complementary method for the early routine monitoring of dactylogyrid infections and for epidemiological studies in which a high number of fish must be studied.

Type
Research Papers
Information
Journal of Helminthology , Volume 92 , Issue 1 , January 2018 , pp. 74 - 80
Copyright
Copyright © Cambridge University Press 2017 

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