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Sequence variation in two mitochondrial DNA regions and internal transcribed spacer among isolates of the nematode Oesophagostomum asperum originating from goats in Hunan Province, China

Published online by Cambridge University Press:  16 September 2014

F. Li ,
T. Hu ,
N.C. Duan ,
W.Y. Li ,
Q. Teng ,
H. Li ,
W. Liu ,
Y. Liu  and
T.Y. Cheng
F. Li
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, 410128, PR China
T. Hu
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, 410128, PR China
N.C. Duan
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, 410128, PR China
W.Y. Li
Affiliation:
Xiang Xi National Vocational Technical College, Jishou, Hunan Province, 416000, PR China
Q. Teng
Affiliation:
Xiang Xi National Vocational Technical College, Jishou, Hunan Province, 416000, PR China
H. Li
Affiliation:
Xiang Xi National Vocational Technical College, Jishou, Hunan Province, 416000, PR China
W. Liu
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, 410128, PR China
Y. Liu*
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, 410128, PR China
T.Y. Cheng*
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, 410128, PR China
*
*Fax: +86 (731) 84673618 E-mail: hn5368@163.com (T.Y.C.); yiliupro@163.com (Y.L.);
*Fax: +86 (731) 84673618 E-mail: hn5368@163.com (T.Y.C.); yiliupro@163.com (Y.L.);
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Abstract

The present study examined sequence variability in two mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1), and internal transcribed spacer (ITS) of nuclear ribosomal DNA (rDNA) among Oesophagostomum asperum isolates from goats in Hunan Province, China. A portion of the cox1 (pcox1), nad1 (pnad1) genes and the ITS (ITS1+5.8S rDNA+ITS2) rDNA were amplified by polymerase chain reaction (PCR) separately from adult O. asperum individuals and the representative amplicons were subjected to sequencing from both directions. The lengths of pcox1, pnad1 and ITS rDNA were 366 bp, 681 bp and 785 bp, respectively. The A+T contents of gene sequences were 71.5–72% for pcox1, 73.7–74.2% for pnad1 and 58–58.8% for ITS rDNA. Intra-specific sequence variations within O. asperum were 0–1.6% for pcox1, 0–1.9% for pnad1 and 0–1.7% for ITS rDNA, while inter-specific sequence differences among members of the genus Oesophagostomum were significantly higher, being 11.1–12.5%, 13.3–17.7% and 8.5–18.6% for pcox1, pnad1 and ITS rDNA, respectively. Phylogenetic analyses using combined sequences of pcox1 and pnad1, with three different computational algorithms (Bayesian inference, maximum likelihood and maximum parsimony), revealed distinct groups with high statistical support. These findings demonstrated the existence of intra-specific variation in mtDNA and rDNA sequences among O. asperum isolates from goats in Hunan Province, China, and have implications for studying molecular epidemiology and population genetics of O. asperum.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 1 , January 2016 , pp. 1 - 6
Copyright
Copyright © Cambridge University Press 2014 

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