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The use of lyophilized Schistosoma mansoni eggs as antigenic particles in a radioimmunoassay

Published online by Cambridge University Press:  05 June 2009

J. Hamburger ,
H. Moscovici  and
S. Lustigman
J. Hamburger
Affiliation:
The Kuvin Centre for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah medical school, Jerusalem, Israel
H. Moscovici
Affiliation:
The Kuvin Centre for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah medical school, Jerusalem, Israel
S. Lustigman
Affiliation:
The Kuvin Centre for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah medical school, Jerusalem, Israel
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Abstract

Lyophilized eggs of Schistosoma mansoni, when incubated briefly with serum from infected mice, bind antibodies, as made evident by subsequent binding of flurorescein labelled anti-IgG or 125 I-labelled protein A. On the basis of these findings, a radioimmunoassay was devised which employs whole lyophilized eggs (500 or 250 eggs/serum sample) as antigenic particles and 125 I-labelled protein A as a probe for antibody binding. Only 10 μl of serum are required to obtain 90% of the maximal binding. Kinetic studies indicated that 70% of the maximal seropositivity develops in mice between five and six weeks after a light infection, reaches a maximum at eight weeks and fluctuates around a high plateau thereafter. Pre-incubation of the test serum with soluble egg antigen (sea) considerably inhibits antibody binding to the eggs, suggesting that SEA-like antigens participate in the reaction.

Type
Research Papers
Information
Journal of Helminthology , Volume 57 , Issue 4 , December 1983 , pp. 305 - 312
Copyright
Copyright © Cambridge University Press 1983

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