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Decisive role of immunocytochemistry in aspiration cytology of chordoma of the clivus: A case report with review of the literature

Published online by Cambridge University Press:  29 June 2007

Giorgio Gherardi ,
Cristina Marveggio ,
Claudio Cola  and
Gianantonio Redaelli
Giorgio Gherardi*
Affiliation:
Department of Pathology, City Hospital of Sondrio, Sondrio, Italy.
Cristina Marveggio
Affiliation:
Department of Pathology, City Hospital of Sondrio, Sondrio, Italy.
Claudio Cola
Affiliation:
Department of Otolaryngology, City Hospital of Sondrio, Sondrio, Italy.
Gianantonio Redaelli
Affiliation:
Department of Otolaryngology, City Hospital of Sondrio, Sondrio, Italy.
*
Dr Giorgio Gherardi, Istituto di Anatomia e Istologia Patologica, Ospedale Civile, 25, Via Stelvio, 23100 Sondrio, Italy.
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Abstract

Immunocytochemistry (ICC) proved to be an essential adjunct in the fine-needle aspiration (FNA) cytological diagnosis of chordoma of the clivus in a 62-year-old woman. The cytological picture in routinely stained smears was not entirely diagnostic for chordoma due to the paucity of typical ‘physalipherous’ cells. To exclude other primary or metastatic neoplasms of the skull base possibly sharing the same cytological picture, additional direct smears were immunostained with antibodies specific for cytokeratin (CK), vimentin (VIM), SI00 protein (SIOOP), carcinoembrionic antigen (CEA), epithelial membrane antigen (EMA), glial fibrillary acidic protein (GFAP), CD68 antigen (KP1) and with the ‘panepithelial’ antibodies B72.3 and Ber-EP4. Chordoma cells showed the following immunoprofile: CK+/VIM−/S100P+/CEA−/EMA+/GFAP−/B72.3−/Ber-EP4−/CD68+. The pattern of immunoreactivity for CK, S100P and CEA confirms previously reported data, while the B72.3−/Ber-EP4−/CD68+ staining profile represents a novel observation. The etection of a CK+/S100+/CEA−/B72.3−/Ber-EP4− immunocytological profile of chordoma cells in aspirates is a basic requirement to exclude pertinent diagnostic differentials, such as metastatic carcinoma, ependymoma and sarcoma, and permits a reliable pre-operative diagnosis of the tumour by aspiration cytology.

Keywords

ImmunohistochemistryBiopsy, needleHead and neck neoplasmsChordoma
Type
Clinical Records
Information
The Journal of Laryngology & Otology , Volume 108 , Issue 5 , May 1994 , pp. 426 - 430
Copyright
Copyright © JLO (1984) Limited 1994

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References

Angelopulos, N., Ceppi, M., Serio, G. (1990) Sacrococcygeal chordoma: a case report. Fine needle aspiration cytologic and histopathologic findings. Pathologica 82: 167–171.Google ScholarPubMed
Apaja-Sarkkinen, M., Vaananen, K., Curran, S., Sipponen, P., Autio-Harmainen, H. (1987) Carcinomatous features of cervical chordoma in a fine needle aspirate. Acta Cytologica 31: 769–773.Google Scholar
Carvalho, G., Coelho, L. H. M. R. (1974) Chordoma of rhinopharynx. Report of a case. Acta Cytologica 18: 425–428.Google ScholarPubMed
De Angelis, M., Buley, I. D., Heryet, A., Gray, W. (1992) Immunocytochemical staining of serous effusions with the monoclonal antibody Ber-EP4. Cytopathology 3: 111–117.CrossRefGoogle ScholarPubMed
Doussis, I. A., Gatter, K. C., Mason, D. Y. (1993) CD68 reactivity of non-macrophage derived tumours in cytological specimens. Journal of Clinical Pathology 46: 334–336.CrossRefGoogle ScholarPubMed
Elliott, E. C., McKinney, A., Banks, H., Fulks, R. M. (1983) Aspiration cytology of metastatic chordoma. A case report. Acta Cytologica 27: 658–662.Google ScholarPubMed
Finley, J. L., Silverman, J. E., Dabbs, D. J., West, R. L., Dickens, A., Feldman, P. S., Frable, W. J. (1986) Chordoma: diagnosis by fine needle aspiration biopsy with histologic, mmunocytochemical, and ultrastructural confirmation. Diagnostic Cytopathology 2: 330–337.CrossRefGoogle ScholarPubMed
Gherardi, G., Marveggio, C. (1992) Immunocytochemistry in head and neck aspirates: diagnostic application on direct smears in 16 problematic cases. Acta Cytologica 36: 687–696.Google ScholarPubMed
Heffelfinger, M. J., Dahlin, D. C., MacCarthy, C. S., Beabout, J. W. (1973) Chordoma and cartilaginous tumors at the skull base. Cancer 32: 410–420.3.0.CO;2-S>CrossRefGoogle ScholarPubMed
Hughes, D. E., Lamb, J., Salter, D. M., Al-Nafussi, A. (1992) Fineneedle aspiration cytology in a case of chordoma. Cytopathology 3: 129–133.CrossRefGoogle Scholar
Kontozoglou, T., Qizilbash, A. H., Sianos, J., Stead, R. (1986) Chordoma: cytologic and immunocytochemical study of four cases. Diagnostic Cytopathology 2: 55–61.CrossRefGoogle ScholarPubMed
Layfield, L. J., Glasgow, B. J., Anders, K. H., Mirra, J. M. (1987) Fine needle aspiration cytology of primary bone lesions. Acta Cytologica 31: 177–184.Google ScholarPubMed
Listrom, M. B., Fenoglio-Preiser, C. M. (1992) Immunocytochemistry in cytology. In Koss Diagnostic Cytology and its Histopathologic Bases. Ch. 34. (Koss, L. G., ed.), J. B. Lippincott Co., Philadelphia, pp 1532–1571.Google Scholar
Micklem, K., Rigney, E., Cordell, J., Simmons, D., Stress, P., Turley, H., Seed, B., Mason, D. (1989) A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies. British Journal of Haematology 73: 6–11.CrossRefGoogle ScholarPubMed
Nance, K. V., Silverman, J. F. (1991) Immunocytochemical panel for the identification of malignant cells in serous effusions. Amerian Journal of Clinical Pathology 95: 867–874.CrossRefGoogle ScholarPubMed
Nijawan, V. S., Rajwanshi, A., Das, A., Jayaram, N., Gupta, S. K. (1989) Fine-needle aspiration cytology of sacrococcygeal chordoma. Diagnostic Cytopathology 5: 404–407.CrossRefGoogle Scholar
O'Dowd, G. J., Schumann, G. B. (1983) Aspiration cytology and cytochemistry of coccygeal chordoma. A case report and review of the literature. Acta Cytologica 27: 178–183.Google ScholarPubMed
Perasole, A., Infantolino, D., Spigariol, F. (1991) Aspiration cytology and immunocytochemistry of sacral chordoma with liver metastases: a case report. Diagnostic Cytopathology 7: 277–281.CrossRefGoogle ScholarPubMed
Perzin, K. H., Pushparaj, N. (1986) Nonepithelial tumors of the nasal cavity, paranasal sinuses, and nasopharynx. A clinicopathologic study. XIV: Chordomas. Cancer 57: 784–796.3.0.CO;2-N>CrossRefGoogle ScholarPubMed
Plate, K. H., Bittinger, A. (1992) Value of immunocytochemistry in aspiration cytology of sacrococcygeal chordoma. A report of two cases. Acta Cytologica 36: 87–90.Google ScholarPubMed
Plaza, J. A., Ballestin, C., Perez-Barrios, A., Martinez, M. A., de Agustin, P. (1989) Cytologic, cytochemical, immunocytochemical and ultrastructural diagnosis of a sacrococcygeal hordoma in a fine needle aspiration biopsy specimen. Acta Cytologica 33: 89–92.Google Scholar
Pulford, K. A. E., Rigney, E. M., Micklem, K. J., Jones, M., Stress, W. P., Gatter, K. C., Mason, D. Y. (1989) KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections. Journal of Clinical Pathology 42: 414–421.CrossRefGoogle ScholarPubMed
Rone, R., Ramzy, I., Duncan, D. (1986) Anaplastic sacrococcygeal chordoma. Fine needle aspiration cytologic findings and embryologic considerations. Acta Cytologica 30: 183–188.Google ScholarPubMed
Walaas, L., Kindblom, L.-G. (1991) Fine-needle aspiration biopsy in the preoperative diagnosis of chordoma: a study of 17 cases with application of electron microscopic, histochemical and immunocytochemical examination. Human Pathology 2: 22–28.CrossRefGoogle Scholar
Xiaojing, P., Xiangcheng, Y. (1985) Cytodiagnosis of bone tumors by fine needle aspiration. Acta Cytologica 29: 570–577.Google Scholar