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Human papillomavirus and salivary gland neoplasia: a p16INK4 immunohistochemical and in situ hybridisation study

Published online by Cambridge University Press:  20 July 2015

M S Miah ,
S Majumdar ,
S White ,
M Robinson  and
N Kernohan
M S Miah*
Affiliation:
Department of ENT – Head and Neck Surgery, Ninewells Hospital and University of Dundee Medical School, Scotland
S Majumdar
Affiliation:
Department of ENT – Head and Neck Surgery, Ninewells Hospital and University of Dundee Medical School, Scotland
S White
Affiliation:
Department of Pathology, Ninewells Hospital and University of Dundee Medical School, Scotland
M Robinson
Affiliation:
Department of Oral Pathology, Centre for Oral Health Research, Freeman Hospital and University of Newcastle upon Tyne, UK
N Kernohan
Affiliation:
Department of Pathology, Ninewells Hospital and University of Dundee Medical School, Scotland
*
Address for correspondence: Mr M S Miah, Department of ENT – Head and Neck Surgery, Ninewells Hospital and University of Dundee Medical School, Dundee DD1 9SY, UK Fax: +44 1382 632816 E-mail: mohammedmiah@nhs.net
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Abstract

Objective:

This study aimed to evaluate the association between human papillomavirus infection and salivary gland tumours in a Scottish cohort.

Methods:

Specimens from a range of salivary gland tumours operated on between 1997 and 2012 were studied. A tissue microarray constructed from tissue blocks was subjected to p16INK4 (cyclin-dependent kinase inhibitor 2A) immunohistochemistry and in situ hybridisation using probes specific for human papillomavirus, including types 16 and 18.

Results:

A total of 61 tumours (benign and malignant) were deemed suitable for the study. p16INK4 staining yielded three (4.9 per cent) positive samples: one small cell carcinoma, one squamous cell carcinoma and one poorly differentiated carcinoma. Human papillomavirus in situ hybridisation demonstrated a positive signal in the latter sample only (1.6 per cent).

Conclusion:

This study demonstrated a very low human papillomavirus detection rate in salivary gland tumours. It can therefore be concluded that human papillomavirus infection is unlikely to play a role in salivary gland neoplasia. Rare human papillomavirus positive cases should be carefully evaluated to exclude the possibility of a metastatic lesion.

Keywords

Salivary GlandsHuman PapillomavirusNeoplasms
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 129 , Issue 10 , October 2015 , pp. 1000 - 1003
Copyright
Copyright © JLO (1984) Limited 2015 

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Footnotes

Presented as an oral presentation at the ENT UK Annual Meeting 2014, 12 September 2014, London, UK

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