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Pars flaccida perforation in tissue culture: morphological study

Published online by Cambridge University Press:  29 June 2007

L.-E. Stenfors ,
E. G. Olsen  and
A. Ö. Henriksen
L.-E. Stenfors*
Affiliation:
Department of Otolaryngology, University of Tromsö, Tromsö, Norway.
E. G. Olsen
Affiliation:
Department of Ophthalmology, University of Tromsö, Tromsö, Norway.
A. Ö. Henriksen
Affiliation:
Department of Otolaryngology, University of Tromsö, Tromsö, Norway.
*
Dr Lars-Eric Stenfors, Idrottsgatan 34, 67200 Karleby, Finland.
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Abstract

Subtotal pars flaccida perforation was effected in vitro in rat tympanic membranes. The drums were subsequently kept in tissue culture and after various intervals studied by means of light and scanning electron microscopy. No complete covering of the perforation was seen, though a marked thickening and hyperplasia of the outer, keratinizing, squamous epithelium (OE) was evident. The inner, tympanal epithelium (IE) appeared swollen, containing rounded structures in the cytoplasm especially close to the contact area with the OE. Ingrowth of OE onto the tympanal side of the drum was seen particularly in the areas where the IE was sparse and completely lacking. Complete covering of a drum perforation seems to be dependent on the formation of supporting granulation tissue, probably resulting from an inflammatory reaction in the healing area.

Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 104 , Issue 2 , February 1990 , pp. 91 - 96
Copyright
Copyright © JLO (1984) Limited 1990

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