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Glycoconjugates in the human fetal endolymphatic sac as detected by lectins

Published online by Cambridge University Press:  29 June 2007

Hiroshi Yamashita ,
Dan Bagger-Sjöbäck ,
Jan Wersäll  and
Toru Sekitani
Hiroshi Yamashita*
Affiliation:
Department of Otolaryngology, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden.
Dan Bagger-Sjöbäck
Affiliation:
Department of Otolaryngology, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden.
Jan Wersäll
Affiliation:
Department of Otolaryngology, Karolinska Hospital, Karolinska Institute, Stockholm, Sweden.
Toru Sekitani
Affiliation:
Department of Otolaryngology, Yamaguchi University School of Medicine, Ube, Japan.
*
Department of Otolaryngology, Yamaguchi University School of Medicine, Ube, Yamaguchi 755, Japan.
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Abstract

The distribution of glycoconjugates in the 11 to 16 weeks old human fetal endolymphatic sac (ES) was analyzed using six biotinylated lectins; Wheat germ agglutinin (WGA), Abrusprecatorius agglutinin (APA), Ulexeuropaeus agglutinin I (UEA-I), Ricinus communis agglutinin 120 (RCA120), Helixpomatia agglutinin (HPA), Concanavalin A (ConA). In the 11 week old human fetus, fluorescent reactions with WGA, APA, RCA120 and ConA were detected in the ES. There was almost no reaction with HPA and UEA-I. In the 14 week old human fetus, however.fluorescent reactions with HPA and UEA-I appeared. This result suggests that the presence of glycoconjugates changes during the maturation process of the ES. Glycoconjugates detected with HPA were related to the epithelial cell elements of the ES epithelium. The reaction with UEA-I suggests that the stainable substance present in the ES lumen may be secreted locally by the ES itself.

Keywords

Endolymphatic sac
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 105 , Issue 9 , September 1991 , pp. 711 - 715
Copyright
Copyright © JLO (1984) Limited 1991

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