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Primary culture of neurospheres obtained from fetal mouse central nervous system and generation of inner ear hair cell immunophenotypes in vitro

Published online by Cambridge University Press:  12 April 2011

X-W Liu  and
J-W Sun
X-W Liu
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Anhui Provincial Hospital, Hefei, Anhui Province, China
J-W Sun*
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Anhui Provincial Hospital, Hefei, Anhui Province, China
*
Address for correspondence: Dr Jingwu Sun, Department of Otolaryngology-Head and Neck Surgery, Anhui Provincial Hospital, Hefei, Anhui Province, China Fax: 86 05512283465 E-mail: sjingwu@hotmail.com.cn
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Abstract

Objective:

To investigate whether hair cell immunophenotypes can be derived from the central nervous system.

Design:

We established in vitro cell cultures from embryonic day 14.5 fetal rat brain tissue, and analysed changes in the immunohistochemical features of these cell cultures following differentiation.

Result:

The immature neural progenitors obtained from the fetal mouse central nervous system generated cell immunophenotypes which expressed epitopes of the hair cell marker proteins myosin VIIa and Brn-3c and the supporting cell marker pan-cytokeratin.

Conclusion:

Neural progenitors have the potential to differentiate into inner ear hair cell and supporting cell phenotypes, and thus may be a useful material for cell transplantation therapy aiming to replace damaged inner ear hair cells.

Keywords

Hearing LossStem CellsCochleaInner Hair CellsNeural Regeneration
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 125 , Issue 7 , July 2011 , pp. 686 - 691
Copyright
Copyright © JLO (1984) Limited 2011

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