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Biocomposites based on collagen and phosphorylated dextran for bone regeneration

Published online by Cambridge University Press:  14 March 2012

M.G. Albu ,
V. Trandafir ,
D.M. Suflet ,
G.C. Chitanu ,
P. Budrugeac  and
I. Titorencu
M.G. Albu*
Affiliation:
Collagen Department, INCDTP—Division Leather and Footwear Research Institute, Bucharest 031215, Romania
V. Trandafir
Affiliation:
Collagen Department, INCDTP—Division Leather and Footwear Research Institute, Bucharest 031215, Romania
D.M. Suflet
Affiliation:
Laboratory of Bioactive and Biocompatible Polymers, “Petru Poni” Institute of Macromolecular Chemistry, Iasi 700487, Romania
G.C. Chitanu
Affiliation:
Laboratory of Bioactive and Biocompatible Polymers, “Petru Poni” Institute of Macromolecular Chemistry, Iasi 700487, Romania
P. Budrugeac
Affiliation:
Laboratory of Determination of Thermal Behaviour of the Solid Products and Materials by Thermal Analysis Methods, National Institute for Research and Development for Electrical Engineering (INCDIE ICPE-CA), Bucharest 030138, Romania
I. Titorencu
Affiliation:
Department of Stem Cell Research and In Vitro Models, Institute of Cellular Biology and Pathology, Bucharest 050568, Romania
*
a)Address all correspondence to this author. e-mail: albu_mada@yahoo.com
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Abstract

The aim of this study was the development of biocomposite scaffolds (membranes and matrices) based on natural polymers used for bone tissue engineering. The novelty featured in this paper is the use of phosphorylated dextran (PDex) as natural component in collagen-based biocomposites. The PDex both in acid form and as mixed salts of Mg–Na, Zn–Na, Ca-Na was characterized by Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR) spectroscopy, potentiometric and conductometric titration and energy dispersive x-ray spectroscopy (EDX) analysis. The biocomposite scaffolds were obtained by freeze-drying as matrices and by free-drying as membranes with specific microporous morphological structures that depended on drying process of collagen gels with PDex. The biocomposites were physical–chemical characterized by differential scanning calorimetry (DSC) and, water and water vapor absorption. The biocompatibility was evaluated in vitro with human osteosarcoma MG 63 cell lines. The results showed that biocompatibility was improved by the use of PDex as mixed salts of Mg–Na, Zn–Na, Ca-Na in collagen biocomposites.

Type
Articles
Information
Journal of Materials Research , Volume 27 , Issue 7 , 14 April 2012 , pp. 1086 - 1096
Copyright
Copyright © Materials Research Society 2012

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