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Deformation of the cell nucleus under indentation: Mechanics and mechanisms

Published online by Cambridge University Press:  01 August 2006

A. Vaziri ,
H. Lee  and
M.R. Kaazempur Mofrad
A. Vaziri*
Affiliation:
Division of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138
H. Lee
Affiliation:
Department of Mechanical Engineering and Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
M.R. Kaazempur Mofrad
Affiliation:
Department of Bioengineering, University of California, Berkeley, California 94720
*
a) Address all correspondence to this author. e-mail: avaziri@deas.harvard.edu
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Abstract

Computational models of the cell nucleus, along with experimental observations, can help in understanding the biomechanics of force-induced nuclear deformation and mechanisms of stress transition throughout the nucleus. Here, we develop a computational model for an isolated nucleus undergoing indentation, which includes separate components representing the nucleoplasm and the nuclear envelope. The nuclear envelope itself is composed of three separate layers: two thin elastic layers representing the inner and outer nuclear membranes and one thicker layer representing the nuclear lamina. The proposed model is capable of separating the structural role of major nuclear components in the force-induced biological response of the nucleus (and ultimately the cell). A systematic analysis is carried out to explore the role of major individual nuclear elements, namely inner and outer membranes, nuclear lamina, and nucleoplasm, as well as the loading and experimental factors such as indentation rate and probe angle, on the biomechanical response of an isolated nucleus in atomic force microscopy indentation experiment.

Keywords

BiologicalNanoscale
Type
Articles
Information
Journal of Materials Research , Volume 21 , Issue 8 , August 2006 , pp. 2126 - 2135
Copyright
Copyright © Materials Research Society 2006

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