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Bone-like growth of hydroxyapatite in the biomimetic coating of Ti-6Al-4V alloy pretreated with protein at 25 °C

Published online by Cambridge University Press:  31 January 2011

Jui Chakraborty ,
Matjaz Mazaj ,
Renu Kapoor ,
S. Pavana Gouri ,
Nina Daneu ,
Mithlesh K. Sinha ,
Gopal Pande  and
Debabrata Basu
Jui Chakraborty*
Affiliation:
Bioceramics and Coating Division, Central Glass and Ceramic Research Institute, Kolkata 700 032, India
Matjaz Mazaj
Affiliation:
National Institute of Chemistry, 1000 Ljubljana, Slovenia
S. Pavana Gouri
Affiliation:
Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
Nina Daneu
Affiliation:
Jozef Stefan Institute, S1-1000 Ljubljana, Slovenia
Mithlesh K. Sinha
Affiliation:
Bioceramics and Coating Division, Central Glass and Ceramic Research Institute, Kolkata 700 032, India
Gopal Pande*
Affiliation:
Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
Debabrata Basu
Affiliation:
Bioceramics and Coating Division, Central Glass and Ceramic Research Institute, Kolkata 700 032, India
*
a) e-mail: jui@cgcri.res.in
b) e-mail: gpande@ccmb.res.in
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Abstract

Commercial-grade dense Ti-6Al-4V alloy substrate was mechanically roughened, cleaned, and treated with a globular protein [bovine serum albumin (BSA)] for 4 h. Biomimetic calcium phosphate (Ca-P) coating was applied onto the above-treated substrate by immersion into simulated body fluid (SBF) at 25 °C for a period of 4 days, with periodic replacement by freshly prepared SBF at 48-h intervals. After 4 days, branched micron-sized fibers of hydroxyapatite (HAp), resembling the structure of bone, were obtained, connecting the clusters of HAp crystal plates in the coating (thickness ∼200 μm) on the substrate surface. Structural and compositional characterization of the coating was carried out using field emission scanning electron microscopy (FE-SEM) with energy-dispersive x-ray analysis unit (EDX) facility, x-ray diffraction (XRD), and Fourier transform infrared (FTIR) data. In vitro cytotoxicity (ISO 10993-5, 1999), cell adhesion assays, and phase contrast microscopy were performed using NIH 3T3 fibroblast cell lines to ascertain the bioactivity of the coated substrates, with and without protein treatment. Based on our study, we propose a correlation between a specific physical structure of the HAp coating and its biological properties.

Keywords

BiomaterialBiomimetic (assembly)Coating
Type
Articles
Information
Journal of Materials Research , Volume 24 , Issue 6 , June 2009 , pp. 2145 - 2153
Copyright
Copyright © Materials Research Society 2009

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