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Nano and micro scale analysis of dentin with in vitro and high speed atomic force microscopy

Published online by Cambridge University Press:  17 June 2013

Yasemin Kutes ,
Varun Vyas  and
Bryan D. Huey
Yasemin Kutes
Affiliation:
Department of Materials Science & Engineering, University of Connecticut, Storrs, Connecticut 06269-3136
Varun Vyas
Affiliation:
Department of Materials Science & Engineering, University of Connecticut, Storrs, Connecticut 06269-3136
Bryan D. Huey*
Affiliation:
Department of Materials Science & Engineering, University of Connecticut, Storrs, Connecticut 06269-3136
*
a)Address all correspondence to this author. e-mail: bhuey@ims.uconn.edu
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Abstract

Atomic force microscopy (AFM) has proven useful in the investigation of porous surfaces due to its nanoscale spatial resolution, micron scale range, compatibility with nonconducting materials, and even applicability ability to biological systems since it can operate in fluids. Since AFM directly measures the surface by contact, it is particularly suited for quantifying the roughness, and more appropriately for porous and particulate materials, the surface area. In this work, a multi-scale porous material, human molar dentin, was studied with AC mode AFM (both in-air and in-liquid), enabling extensive analyses both for plain dentin as well as specimens exposed to nanoparticle TiO2 containing toothpaste to approximate personal dental hygiene. Finally, high speed AFM is also demonstrated in vitro with equivalent results, except that the time required per image is reduced by several orders of magnitude from tens of minutes to as little as 6 s. Careful implementation of AFM, both at standard and high speeds, is therefore effective for investigating highly porous materials, including biological tissue, in environmentally or physiologically relevant conditions.

Type
Articles
Information
Journal of Materials Research , Volume 28 , Issue 17: Focus Issue: Advances in the Synthesis, Characterization, and Properties of Bulk Porous Materials , 14 September 2013 , pp. 2300 - 2307
Copyright
Copyright © Materials Research Society 2013 

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References

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