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Numerical Simulation of Separation of Circulating Tumor Cells from Blood Stream in Deterministic Lateral Displacement (DLD) Microfluidic Channel

Published online by Cambridge University Press:  21 December 2015

F. Khodaee ,
S. Movahed ,
N. Fatouraee  and
F. Daneshmand
F. Khodaee
Affiliation:
Biological Fluid Mechanics Research LaboratoryBiomechanics DepartmentAmirkabir University of TechnologyTehran, Iran
S. Movahed*
Affiliation:
Department of Mechanical EngineeringAmirkabir University of TechnologyTehran, Iran
N. Fatouraee
Affiliation:
Biological Fluid Mechanics Research LaboratoryBiomechanics DepartmentAmirkabir University of TechnologyTehran, Iran
F. Daneshmand
Affiliation:
Department of Mechanical EngineeringMcGill UniversityMontreal, Canada Department of Bioresource EngineeringMcGill UniversityMontreal, Canada
*
*Corresponding author (smovahed@aut.ac.ir)

Abstract

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Deterministic Lateral Displacement (DLD) microfluidic devices provide a reliable label-free separation method for detection of circulating tumor cells (CTCs) in blood samples based on their biophysical properties. In this paper, we proposed an effective design of the DLD microfluidic device for the CTC separation in the blood stream. A typical DLD array is designed and numerical simulations are performed to separate the CTC and leukocyte (white blood cells) in different fluid flow conditions. Fluid-Solid Interaction method is used to investigate the behaviour of these deformable cells in fluid flow. In this study, the effects of critical parameters affecting cell separation in the DLD microfluidic devices (e.g. flow condition, cell deformability, and stress) have been investigated. The obtained results show that unlike leukocytes, the CTC’s motion is independent of the flow condition and is laterally displaced even in higher Reynolds number. Larger cells (CTCs) cannot intercept the low-velocity fluid near the wall of the posts; thus, they move faster and become separated from leukocytes. To reduce the cellular stress during separation process, which causes increase of cell viability and more effective design of microfluidic device, the results obtained here may be used as a significant design parameter for the DLD fabrication.

Keywords

Deterministic lateral displacement (DLD)Circulating tumor cell (CTC)MicrofluidicsFluid-solid interaction (FSI)
Type
Research Article
Information
Journal of Mechanics , Volume 32 , Issue 4 , August 2016 , pp. 463 - 471
Copyright
Copyright © The Society of Theoretical and Applied Mechanics 2016 

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