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Di Brom Thymol Sulhpone Phthalein as a Reagent for Determining the Hydrogen Ion Concentration of Living Cells

Published online by Cambridge University Press:  11 May 2009

W. R. G. Atkins
W. R. G. Atkins
Affiliation:
Head of the Department of General Physiology at the Plymouth Laboratory.
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Extract

1. Brom thymol blue may be used in dilute solution for ascertaining the hydrogen ion concentration of certain marine organisms. It penetrates slowly, but the stained portions remain actively motile, so its toxic action does not appear to be great at the dilutions found serviceable.

2. The animals studied gave values from pH6·2 to about pH7·5, though possibly the more alkaline end of the range may be pathological. About pH0·2 should be subtracted from these figures for neutral salt error. The sea water used was initially at pH8·2, corrected.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 12 , Issue 4 , October 1922 , pp. 781 - 784
Copyright
Copyright © Marine Biological Association of the United Kingdom 1922

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References

Clark, W. M. 1920. The determination of hydrogen ions. Baltimore.Google Scholar
Crozier, W. J. 1912–1919. See Clark, also collected papers reprinted in Contributions from the Bermuda Biol. Sta., Vols. III and V.Google Scholar
Ehrlich, P., etc. 1910. Enzyklopädie d. mikroskop. Technik, 2, 589. Berlin.Google Scholar
Harvey, E. N. 1914. The relation between the rate of penetration of marine tissues by alkali and the change in functional activity induced by the alkali. Carnegie Inst. Wash., Publ. No. 183. Tortugas Lab. Papers, Vol. VI.Google Scholar
Harvey, E. N. 1915. The permeability of cells for acids. Loc. cit. No. 212 and Vol. VIII.Google Scholar
Heidenhain, M. 1907. Plasma und Zelle. Die Vitalfarbstoffe, s. 436. Jena.Google Scholar
Lee, A. B. 1921. The microtomist's vade-mecum. 8th Ed. London.Google Scholar