Hostname: page-component-84b7d79bbc-l82ql Total loading time: 0 Render date: 2024-07-29T12:37:13.085Z Has data issue: false hasContentIssue false
  • English
  • Français

Dissolved organic matter release by an axenic culture of Emiliania huxleyi

Published online by Cambridge University Press:  22 July 2008

Suhaimi Suratman ,
Keith Weston ,
Tim Jickells ,
Rosie Chance  and
Tom Bell
Suhaimi Suratman*
Affiliation:
School of Environmental Sciences, University of East Anglia, Norwich, Norfolk NR4 7TJ, UK Present address: Environmental Research Group, Department of Chemical Sciences, University Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia
Keith Weston
Affiliation:
School of Environmental Sciences, University of East Anglia, Norwich, Norfolk NR4 7TJ, UK
Tim Jickells
Affiliation:
School of Environmental Sciences, University of East Anglia, Norwich, Norfolk NR4 7TJ, UK
Rosie Chance
Affiliation:
School of Environmental Sciences, University of East Anglia, Norwich, Norfolk NR4 7TJ, UK Present address: Department of Chemistry, University of York, Heslington, York YO10 5DD, UK
Tom Bell
Affiliation:
School of Environmental Sciences, University of East Anglia, Norwich, Norfolk NR4 7TJ, UK
*
Correspondence should be addressed to: Suhaimi Suratman, Environmental Research Group, Department of Chemical Sciences, University of Malaysia Terengganu, 21030 Kuala Terengganu, Terrengganu, Malaysia email: miman@umt.edu.my
Get access Rights & Permissions [Opens in a new window]

Abstract

Measurements of the release of dissolved organic nitrogen (DON) and carbon (DOC) were carried out on an axenic batch culture of the coccolithophorid Emiliania huxleyi. This unicellular marine alga was cultured using a media with nitrate as the sole N source and the changes of DOM concentrations measured over 14 days. Results showed that there was a significant release of DON, i.e.7.6 µM N day−1 during mid-exponential growth phase (days 5–7). The highest release of DOC was also recorded in the same growth phase and accounted for 24.0 μM C day−1.

Keywords

dissolved organic matteranexic cultureEmiliana huxleyi
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 88 , Issue 7 , November 2008 , pp. 1343 - 1346
Copyright
Copyright © Marine Biological Association of the United Kingdom 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Aluwihare, L.I. and Repeta, D.J. (1999) A comparison of the chemical characteristics of oceanic DOM and extracellular DOM produced by marine algae. Marine Ecology Progress Series 186, 105–117.CrossRefGoogle Scholar
Arrigo, K.R. (2005) Marine microorganisms and global nutrient cycles. Nature 437, 349–355.CrossRefGoogle ScholarPubMed
Berman, T. and Chava, S. (1999) Algal growth on organic compounds as nitrogen sources. Journal of Plankton Research 21, 1423–1437.CrossRefGoogle Scholar
Carpenter, E.J., Remsen, C.C. and Watson, S.W. (1972) Utilization of urea by some marine phytoplankters. Limnology and Oceanography 17, 265–269.CrossRefGoogle Scholar
Chance, R., Malin, G., Jickells, T. and Baker, A.R. (2007) Reduction of iodate to iodide by cold water diatom cultures. Marine Chemistry 105, 169–180.CrossRefGoogle Scholar
Collos, Y. (1992) Nitrogen budgets and dissolved organic matter cycling. Marine Ecology Progress Series 90, 201–206.CrossRefGoogle Scholar
Collos, Y., Dohler, G. and Biermann, I. (1992) Production of dissolved organic nitrogen during uptake of nitrate by Synedra planctonica: implications for estimates of new production in the oceans. Journal of Plankton Research 14, 1025–1029.CrossRefGoogle Scholar
Conover, S.A.M. (1975) Partitioning of nitrogen and carbon in cultures of the marine diatom Thalassiosira fluviatilis supplied with nitrate, ammonium or urea. Marine Biology 32, 231–246.CrossRefGoogle Scholar
Engel, A., Delille, B., Jacquet, S., Riebesell, U., Rochelle-Newall, E., Terbruggen, A. and Zondervan, I. (2004) Transparent exopolymer particles and dissolved organic carbon production by Emiliania huxleyi exposed to different CO2 concentrations: a mesocosm experiment. Aquatic Microbial Ecology 34, 93–104.CrossRefGoogle Scholar
Fogg, G.E. (1977) Excretion of organic matter by phytoplankton. Limnology and Oceanography 22, 576–577.CrossRefGoogle Scholar
Gobler, C.J. and Sanudo-Wilhelmy, S.A. (2003) Cycling of colloidal organic carbon and nitrogen during an estuarine phytoplankton bloom. Limnology and Oceanography 48, 2314–2320.CrossRefGoogle Scholar
Guillard, R.R.L. (1975) Culture of phytoplankton for feeding marine invertebrates. In Smith, W.L. and Chanley, M.H. (eds) Culture of marine invertebrate animals. New York: Plenum Press, pp. 29–60.CrossRefGoogle Scholar
Hansell, D.A., Williams, P. and Ward, B.B. (1993) Measurements of DOC and DON in the Southern California Bight using oxidation by high temperature combustion. Deep-Sea Research I 40, 219–234.CrossRefGoogle Scholar
Holmes, R.M., Aminot, A.Keroeul, R., Hooker, B.A. and Peterson, B.J. (1999) A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Canadian Journal of Fisheries and Aquatic Sciences 56, 1801–1808.CrossRefGoogle Scholar
Hygum, B.H., Petersen, J.W. and Sondergaard, M. (1997) Dissolved organic carbon released by zooplankton grazing activity—a high-quality substrate pool for bacteria. Journal of Plankton Research 19, 97–111.CrossRefGoogle Scholar
Kirchman, D.L., Suzuki, Y., Garside, C. and Ducklow, H.W. (1991) High turnover rates of dissolved organic carbon during a spring phytoplankton bloom. Nature 352, 612–614.CrossRefGoogle Scholar
Kirkwood, D.S. (1996) Nutrients: practical notes on their determination in seawater. ICES Techniques in Marine Environmental Sciences. No. 17. Copenhagen: International Council for the Exploration of the Seas, 23 pp.Google Scholar
Lampert, W. (1978) Release of dissolved organic carbon by grazing zooplankton. Limnology and Oceanography 23, 831–834.CrossRefGoogle Scholar
Mulholland, M.R., Bronk, D.A. and Capone, D.G. (2004) Dinitrogen fixation and release of ammonium and dissolved organic nitrogen by Trichodesmium IMS101. Aquatic Microbial Ecology 37, 85–94.CrossRefGoogle Scholar
Myklestad, S., Holm-Hansen, O., Varum, K.M. and Volcani, B.E. (1989) Rate of release of extracellular amino acids and carbohydrates from the marine diatom Chaetoceros affinis. Journal of Plankton Research 11, 763–773.CrossRefGoogle Scholar
Newell, B.S., Dalpont, G. and Grant, B.R. (1972) The excretion of organic nitrogen by marine algae in batch and continuous culture. Canadian Journal of Botany 50, 2605–2611.CrossRefGoogle Scholar
Painter, S.C., Sanders, R., Poulton, A.J., Woodward, E.M.S., Lucas, M. and Chamberlain, K. (2007) Nitrate uptake at photic zone depths is not important for export in the subtropical ocean. Global Biogeochemical Cycles 21, GB4005, doi:10.1029/2006GB002807.CrossRefGoogle Scholar
Parsons, T.R., Maita, Y. and Lalli, C.M. (1984) A manual of chemical and biological methods for seawater analysis. Oxford: Pergamon Press.Google Scholar
Pujo-Pay, M., Conan, P. and Raimbault, P. (1997) Excretion of dissolved organic nitrogen by phytoplankton assessed by wet oxidation and 15N tracer procedures. Marine Ecology Progress Series 153, 99–111.CrossRefGoogle Scholar
Redfield, A.C., Ketchum, B.H. and Richard, F.A. (1966) The influence of organisms on the composition of sea-water. In Hill, M.N. (ed.) The sea. New York: Wiley Interscience, pp. 26–77.Google Scholar
Sharp, J.H. (1977) Excretion of organic matter by marine phytoplankton: do healthy cells do it? Limnology and Oceanography 22, 381–399.CrossRefGoogle Scholar
Sharp, J.H. (2002) Analytical methods for total DOM pools. In Hansell, D.A. and Carlson, C.A. (eds) Biogeochemistry of marine dissolved organic matter. San Diego: Academic Press, pp. 35–58.CrossRefGoogle Scholar
Sherr, E.B., Caron, D.A. and Sherr, B.F. (1993) Staining of heterotrophic protists for visualization via epifluorescence microscopy. In Kemp, P.F., Sherr, B.F., Sherr, E.B. and Cole, J.J. (eds) Handbook of methods in aquatic microbial ecology. Florida: Lewis Publishers, pp. 213–227.Google Scholar
Slawyk, G. and Raimbault, P. (1995) Simple procedure for simultaneous recovery of dissolved inorganic and organic nitrogen in 15N-tracer experiments and improving the isotopic mass balance. Marine Ecology Progress Series 124, 289–299.CrossRefGoogle Scholar
Suratman, S. (2007) The seasonal distribution and cycling of nitrogen and organic carbon-based nutrients in the North Sea. PhD thesis. University of East Anglia, Norwich, UK.Google Scholar
Vogel, S.L. and Frisch, H.L. (1978) Qualitative assay of dissolved amino acids and sugars excreted by Chlamydomonas reinhardth (Chlorophyceae) and Euglena gracilis (Euglenophyceae). Journal of Phycology 14, 403–406.CrossRefGoogle Scholar
Winter, A., Jordan, R.W. and Roth, P.H. (1994) Biogeography of coccolithophores in ocean waters. In Winter, A. and Siesser, W.G. (eds) Coccolithophores. Cambridge: Cambridge University Press, pp. 161–177.Google Scholar