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Seasonal changes in the phytoplankton as indicated by chlorophyll estimations

Published online by Cambridge University Press:  11 May 2009

W. R. G. Atkins
Affiliation:
From the Plymouth Laboratory
Mary Parke
Affiliation:
From the Plymouth Laboratory

Extract

The pigments of pure cultures of algae, of known number per volume, have been extracted with aqueous acetone after separation by filtration. The extracts were compared in a Kober colorimeter with standard solutions of a preparation of bought chlorophyll. The number required to give 1 mg. of chlorophyll was thus determined.

The algae studied included: Chlorophyceae, two species of Chlamydomonas and one Chlorella not yet described; Chrysophyceae, Dicrateria inornata Parke; Bacillariophyceae, Coscinodiscus centralis Ehr., Thalassiosira gravida Cleve and Nitzschia closterium (Ehr.) W.Sm. forma minutissima; Cryptophyceae, Hemiselmis rufescens Parke; Dinophyceae, Gymnodinium sp. The numbers per milligram of chlorophyll varied from 74 × 103 for Coscinodiscus to over 3200 × 106 for Hemiselmis rufescens. Of the nine cultures, six were between 50 and 750 × 106. The volume of the cell is the decisive factor in the milligram count.

Type
Research Article
Copyright
Copyright © Marine Biological Association of the United Kingdom 1951

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