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Zinc transport in the haemolymph of Carcinus maenas (Crustacea: Decapoda)

Published online by Cambridge University Press:  11 May 2009

Paolo Zatta
Paolo Zatta
Affiliation:
C.N.R.-Centro di studio per la Fisiologia e la Biochimica delle Emocianine e di altre Metallo-Proteine, Via Loredan 10, 35131 Padova, Italy
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Abstract

In Carcinus maenas haemolymph, zinc is almost entirely bound to the respiratory pigment, which is the copper-protein haemocyanin (Hc). Zinc ions are loosely bound, as indicated by the low value of the association constant (k = 1.7 × 105 M-1 at pH = 8.0). The number of binding sites N is equal to 4 per minimal functional subunit (75000 Dalton). No co-operativity has been found between the different metal sites. Data reported in this paper support the hypothesis that haemocyanin can act as metal carrier in the haemolymph of C. maenas.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 64 , Issue 4 , November 1984 , pp. 801 - 807
Copyright
Copyright © Marine Biological Association of the United Kingdom 1984

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References

Adams, E., Simkiss, K. & Taylor, M., 1982. Metal ion metabolism in the moulting crayfish (Austropotamobius pallipes). Comparative Biochemistry and Physiology, 72A, 73–76.CrossRefGoogle Scholar
Arvy, L., 1969. Les enzymes chez les crustaces. Anneée biologique, 8, 505–580.Google Scholar
Bryan, G. W., 1967. Zinc concentrations of fast and slow contracting muscles in the lobster. Nature, London, 213, 1043–1044.CrossRefGoogle Scholar
Bryan, G. W., 1968. Concentrations of zinc and copper in the tissues of decapod crustaceans. Journal of the Marine Biological Association of the United Kingdom, 48, 303–321.CrossRefGoogle Scholar
Bryan, G. W., 1971. The effects of heavy metals (other than mercury) on marine and estuarine organisms. Proceedings of the Royal Society (B), 177, 389–410.Google ScholarPubMed
Colvocoresses, J. A. & Lynch, M. P., 1975. Variations in serum constituents of the blue crab, Callinectes sapidus: copper and zinc. Comparative Biochemistry and Physiology, 50A, 135–139.CrossRefGoogle ScholarPubMed
Gilles, R., 1979. Intracellular organic osmotic effector. In Mechanism of Osmoregulation in Animals (ed. Gilles, R.), pp. 111–154. J. Wiley.Google Scholar
Martin, J. L. M., Van Wormhoudt, A. & Ceccaldi, H. J., 1977. Zinc-hemocyanin binding in the hemolymph of Carcinus maenas (Crustacea, Decapoda). Comparative Biochemistry and Physiology, 58A, 193–195.CrossRefGoogle Scholar
Scatchard, G., 1949. The attractions of proteins for small molecules and ions. Annals of the New York Academy of Sciences, 51, 660–672.CrossRefGoogle Scholar
Vallee, L. B., 1978. New Trends in Bio-inorganic Chemistry. 21 pp. Academic Press.Google Scholar
Wright, D. A., 1977. The uptake of cadmium into the haemolymph of the shore crab Carcinus maenas: the relationship with copper and other divalent cations. Journal of Experimental Biology, 67, 147–161.CrossRefGoogle Scholar
Zatta, P., Moschini, G., Buso, P., Colautti, P. & Stievano, B. M., 1983. Hemocyanin as metal transport in Carcinus maenas blood. In The Invertebrate Respiratory Pigment (ed. Wood, E.), pp. 333–334. Harwood.Google Scholar
Zatta, P. & Salvato, B., 1981. Reconstitution of Carcinus maenas hemocyanin in the presence of nonionic detergent. Journal of Inorganic Biochemistry, 15, 269–273.CrossRefGoogle Scholar