The morphology of Genipa americana seeds and post-seminal development was studied, and its germination response investigated in relation to storage in water, substrate water volume, osmotic potential and temperature and light regimes. The seeds are flattened and seedlings are phaneroepigeal with leafy cotyledons. Seeds stored in water kept viable for up to 6 mo, but germination and seedling emergence were reduced after 4 mo. Germination was not affected under water-logged conditions, but seedling emergence was reduced at the water volume corresponding to a water column two times deeper than the seeds' mean thickness (70 ml). When seeds were osmotically stressed, a reduction in the rate and germination percentage occurred at −0.2 MPa and germination was drastically inhibited at −0.6 MPa. Seeds germinated in a wide range of temperatures (15–40 °C), with optimum at continuous temperatures of 25 and 30 °C. Light was not required for germination, and no dormancy mechanisms were observed. The seed and seedling morphology and the germinative responses of the species may permit its regeneration in periodically flooded lowland forests and riparian habitats and are probably key factors in explaining the species' wide distribution.