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Actin type I introns offer potential for increasing phylogenetic resolution in Asterochloris (Chlorophyta: Trebouxiophyceae)

Published online by Cambridge University Press:  22 August 2006

Matthew P. NELSEN  and
Andrea GARGAS
Matthew P. NELSEN
Affiliation:
Department of Botany, University of Wisconsin-Madison, Madison, Wisconsin, 53706-1381, USA Biotechnology Research Centre, School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, Michigan, 49931-1295, USA
Andrea GARGAS
Affiliation:
Department of Botany, University of Wisconsin-Madison, Madison, Wisconsin, 53706-1381, USA
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Abstract

Primers are presented to amplify an intron-containing portion of the actin type I locus from Asterochloris algae. The amount of variation and phylogenetic resolution provided by the ITS rDNA are compared with that from the actin locus. The number of variable and parsimony-informative sites within the actin locus was drastically higher than that of the ITS. Phylogenetic analyses of the ITS resulted in poorly resolved trees with low support values. In contrast, phylogenetic analyses of the actin locus yielded trees with better support and resolution. It is concluded that the actin type I introns will be useful for studies investigating species concepts and population genetics.

Keywords

actinalgaeAsterochlorisITS rDNAlichenphylogenetic analyses
Type
Research Article
Information
The Lichenologist , Volume 38 , Issue 5 , September 2006 , pp. 435 - 440
Copyright
© British Lichen Society 2006

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